Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

HSPB3 ELISA Kit

HSPB3 Reactivity: Human Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Tissue Homogenate
Catalog No. ABIN5654991
  • Target See all HSPB3 ELISA Kits
    HSPB3 (Heat Shock 27kDa Protein 3 (HSPB3))
    Reactivity
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    0.781 ng/mL - 50 ng/mL
    Minimum Detection Limit
    0.781 ng/mL
    Application
    ELISA
    Sample Type
    Tissue Homogenate
    Analytical Method
    Quantitative
    Specificity
    This assay has high sensitivity and excellent specificity for detection of Heat Shock Protein Beta 3 (HSPb3). No significant cross-reactivity or interference between Heat Shock Protein Beta 3 (HSPb3) and analogues was observed.
    Sensitivity
    0.29 ng/mL
    Featured
    Discover our best selling HSPB3 ELISA Kit
    Top Product
    Discover our top product HSPB3 ELISA Kit
  • Comment

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Assay Time
    3 h
    Plate
    Pre-coated
    Protocol
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock Protein Beta 3 (HSPb3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heat Shock Protein Beta 3 (HSPb3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock Protein Beta 3 (HSPb3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heat Shock Protein Beta 3 (HSPb3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Assay Precision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Heat Shock Protein Beta 3 (HSPb3) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Heat Shock Protein Beta 3 (HSPb3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Handling Advice
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Storage
    4 °C,-20 °C
    Storage Comment
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Expiry Date
    4-8 months
  • Target See all HSPB3 ELISA Kits
    HSPB3 (Heat Shock 27kDa Protein 3 (HSPB3))
    Alternative Name
    Heat Shock Protein Beta 3 (HSPB3 Products)
    Synonyms
    HSPB3 ELISA Kit, DHMN2C ELISA Kit, HMN2C ELISA Kit, HSPL27 ELISA Kit, 2310035K17Rik ELISA Kit, AI844863 ELISA Kit, Hsbp3 ELISA Kit, spb3 ELISA Kit, heat shock protein family B (small) member 3 ELISA Kit, heat shock 27kDa protein 3 ELISA Kit, heat shock protein 3 ELISA Kit, HSPB3 ELISA Kit, hspb3 ELISA Kit, Hspb3 ELISA Kit
    Background

    Gene Name: Heat Shock Protein Beta 3

    Gene Aliases: HSP-B3, HSPL27, HSP27, HSP 17, Heat Shock 27 kDa Protein 3, Heat shock 17 kDa protein

    Gene ID
    8988
    UniProt
    Q12988
You are here:
Support