Parkin ELISA Kit

Catalog No. ABIN5657641

Product Details Parkin ELISA Kit

Target: Parkin (PARK2) (Parkinson Protein 2, E3 Ubiquitin Protein Ligase (Parkin) (PARK2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Parkinson Disease Protein 2 (PARK2). No significant cross-reactivity or interference between Parkinson Disease Protein 2 (PARK2) and analogues was observed.

Sensitivity: 0.053 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Parkinson Disease Protein 2 (PARK2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Parkinson Disease Protein 2 (PARK2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Parkinson Disease Protein 2 (PARK2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Parkinson Disease Protein 2 (PARK2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Parkinson Disease Protein 2 (PARK2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Parkinson Disease Protein 2 (PARK2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for Parkin

Target: Parkin (PARK2) (Parkinson Protein 2, E3 Ubiquitin Protein Ligase (Parkin) (PARK2))

Alternative Name: Parkinson Disease Protein 2 (PARK2 Products)

Synonyms: CG10523 ELISA Kit, Dmel\\CG10523 ELISA Kit, Dpark ELISA Kit, SD01679 ELISA Kit, dpk ELISA Kit, AR-JP ELISA Kit, LPRS2 ELISA Kit, PDJ ELISA Kit, PRKN ELISA Kit, Park ELISA Kit, Prkn ELISA Kit, si:ch211-123f21.1 ELISA Kit, zgc:112390 ELISA Kit, pdr-1 ELISA Kit, PARK2 ELISA Kit, parkin ELISA Kit, parkin RBR E3 ubiquitin protein ligase ELISA Kit, Parkinson disease (autosomal recessive, juvenile) 2, parkin ELISA Kit, park ELISA Kit, PRKN ELISA Kit, Prkn ELISA Kit, prkn ELISA Kit, CpipJ_CPIJ014867 ELISA Kit, Park2 ELISA Kit

Background:

Gene Name: Parkinson Disease Protein 2

Gene Aliases: AR-JP, LPRS2, PDJ, PRKN, Parkin, Autosomal Recessive,Juvenile, E3 ubiquitin-protein ligase parkin

Gene ID: 5071

UniProt: O60260

Pathways: Autophagy, Ubiquitin Proteasome Pathway