NAMPT ELISA Kit

Catalog No. ABIN5659948

Product Details NAMPT ELISA Kit

Target: NAMPT (Nicotinamide phosphoribosyltransferase (NAMPT))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.56 ng/mL - 100 ng/mL

Minimum Detection Limit: 1.56 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Visfatin (VF). No significant cross-reactivity or interference between Visfatin (VF) and analogues was observed.

Sensitivity: 0.58 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Visfatin (VF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Visfatin (VF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Visfatin (VF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Visfatin (VF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Visfatin (VF) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Visfatin (VF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for NAMPT

Target: NAMPT (Nicotinamide phosphoribosyltransferase (NAMPT))

Alternative Name: Visfatin (NAMPT Products)

Synonyms: 1110035O14Rik ELISA Kit, PBEF ELISA Kit, PBEF1 ELISA Kit, VF ELISA Kit, VISFATIN ELISA Kit, AI314458 ELISA Kit, AI480535 ELISA Kit, NAmPRTase ELISA Kit, Pbef ELISA Kit, Pbef1 ELISA Kit, Visfatin ELISA Kit, visfatin ELISA Kit, pbef ELISA Kit, pbef1 ELISA Kit, nicotinamide phosphoribosyltransferase ELISA Kit, Nicotinamide phosphoribosyltransferase ELISA Kit, nicotinamide phosphoribosyltransferase S homeolog ELISA Kit, NAMPT ELISA Kit, Nampt ELISA Kit, nampt ELISA Kit, Smon_0680 ELISA Kit, Mrub_2845 ELISA Kit, Cseg_3704 ELISA Kit, BC1002_3997 ELISA Kit, Ftrac_2686 ELISA Kit, Varpa_5681 ELISA Kit, Celal_3900 ELISA Kit, Deima_0880 ELISA Kit, BC1001_3724 ELISA Kit, Celly_1696 ELISA Kit, Clole_1542 ELISA Kit, Tsp_05934 ELISA Kit, nampt.S ELISA Kit

Background:

Gene Name: Visfatin

Gene Aliases: Nampt, NAmPRTase, PBEF, PBEF1, Pre-B-Cell Colony Enhancing Factor, Nicotinamide Phosphoribosyltransferase

Gene ID: 297508

UniProt: Q80Z29