SIRT2 ELISA Kit

Catalog No. ABIN5674759

Product Details SIRT2 ELISA Kit

Target: SIRT2 (Sirtuin 2 (SIRT2))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.56 ng/mL - 100 ng/mL

Minimum Detection Limit: 1.56 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Sensitivity: 0.61 ng/mL

Components: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Material not included: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Assay Time: 1 - 4.5 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12 months

Target Details for SIRT2

Target: SIRT2 (Sirtuin 2 (SIRT2))

Abstract: SIRT2 Products

Synonyms: CG5085 ELISA Kit, D.mel2 ELISA Kit, Dmel\\CG5085 ELISA Kit, SIRT2 ELISA Kit, dSIRT2 ELISA Kit, sir2 ELISA Kit, Sirt2 ELISA Kit, BmSirt2 ELISA Kit, AtSRT2 ELISA Kit, SIRTUIN 2 ELISA Kit, T2K12.8 ELISA Kit, sirtuin 2 ELISA Kit, SIR2 ELISA Kit, SIR2L ELISA Kit, SIR2L2 ELISA Kit, 5730427M03Rik ELISA Kit, Sir2l ELISA Kit, wu:fb57d05 ELISA Kit, zgc:55966 ELISA Kit, zgc:77003 ELISA Kit, Sirtuin 2 ELISA Kit, sirtuin 2 ELISA Kit, sirtuin 2 S homeolog ELISA Kit, NAD-dependent deacetylase sirtuin 2 homolog ELISA Kit, sirtuin 2 (silent mating type information regulation 2, homolog) 2 (S. cerevisiae) ELISA Kit, Sirt2 ELISA Kit, SIRT2 ELISA Kit, sirt2.S ELISA Kit, SRT2 ELISA Kit, sirt2 ELISA Kit

Background: Alternative name: SIR2L, SIR2-L, SIR2L2, Silent Information Regulator 2 Protein, NAD-Dependent Deacetylase Sirtuin-2, Silent Mating Type Information Regulation 2 Homolog

Gene ID: 64383

UniProt: Q8VDQ8