Asparaginase Activity Assay Kit

Antigen: Asparaginase

Catalog no.: ABIN593353

Additional Information

Characteristics: Detection method- Absorbance (570 nm) or Fluorescence (Ex/Em 535/590 nm), Sample type- Bacteria, plants and many animals (e.g. guinea pigs) cell lysates, biological samples, cancer cell lysates, food samples etc. Features: Simple procedure, takes 40 minutes, Fast, reliable and convenient, Kit can be used to detect Asparaginase enzymeﾒs antineoplastic effects, based on the inability of cancer cells (unlike healthy cells) to synthesize asparagine.

Description: Asparaginase (EC 3.5.1.1) is a homotetramer that catalyzes the hydrolysis of asparagine to aspartic acid and ammonia and exhibits about a 2-4% activity on glutamine and 5% on D-asparagine. Asparaginase does not occur naturally in humans but is found in bacteria, plants and many animals (e.g. guinea pigs). The enzyme has been used to reduce acrylamide, a suspected carcinogen, produced in fried starchy food products and to treat acute lymphoblastic leukemia (ALL) and some other hematopoietic neoplasms (e.g. multiple myeloma). Metabolization of asparagine prevents acrylamide formation in fried foods (Maillard reaction). The enzymeﾒs antineoplastic effects are based on the inability of cancer cells (unlike healthy cells) to synthesize asparagine. However, the enzyme is not without some antigenicity and toxicity so it is very important to measure its activity in biological samples or monitor its activity during therapy. BioVision provides a simple, direct and automation-ready procedure for measuring asparaginase activity in biological samples. In the assay, Asparaginase hydrolyzes asparagine to generate aspartic acid, which can be detected colorimetrically (lambda=570 nm) or fluorescently (Ex/Em = 535/590 nm) using a coupled enzymatic reaction. Keywords: Asparaginase, Asparaginase kit, Asparaginase assay

Application Details

Components: Asparaginase Assay Buffer, OxiRedﾙ Probe (in DMSO), Substrate Mix, Aspartate Enzyme Mix, Conversion Mix, Positive Control, Aspartate Standard (100 mM)

Storage: Store at: -20°C, Shipping Conditions: gel pack, Shelf Life: 12 months

Research Area: Signaling, Metabolism, Amino Acids, Enzymes

Restrictions: For Research Use only