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CCL1 ELISA Kit

CCL1 Reactivity: Human Colorimetric Sandwich ELISA 4-3000 pg/mL Cell Culture Supernatant, Plasma, Serum
Catalog No. ABIN624994
  • Target See all CCL1 ELISA Kits
    CCL1 (Chemokine (C-C Motif) Ligand 1 (CCL1))
    Reactivity
    • 6
    • 5
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    4-3000 pg/mL
    Minimum Detection Limit
    4 pg/mL
    Application
    ELISA
    Purpose
    Human I-309 (TCA-3/CCL1) ELISA Kit for cell culture supernatants, plasma, and serum samples.
    Sample Type
    Plasma, Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    Specificity
    This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, MMP-1, - 2, -3, -10, PARC, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
    Sensitivity
    4 pg/mL
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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    Discover our top product CCL1 ELISA Kit
  • Application Notes
    Recommended Dilution for serum and plasma samples2 fold
    Sample Volume
    100 μL
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Expiry Date
    6 months
  • Das, Sarrou, Podgrabinska, Cassella, Mungamuri, Feirt, Gordon, Nagi, Wang, Entenberg, Condeelis, Skobe: "Tumor cell entry into the lymph node is controlled by CCL1 chemokine expressed by lymph node lymphatic sinuses." in: The Journal of experimental medicine, Vol. 210, Issue 8, pp. 1509-28, (2013) (PubMed).

    Leow, Goh, Heng: "Effect of serum amyloid A1 treatment on global gene expression in THP-1-derived macrophages." in: Inflammation research : official journal of the European Histamine Research Society ... [et al.], Vol. 61, Issue 4, pp. 391-8, (2012) (PubMed).

    Celebi, Mantovani, Pineault: "Irradiated Mesenchymal Stem Cells improve the ex vivo expansion of Hematopoietic Progenitors by partly mimicking the bone marrow endosteal environment." in: Journal of immunological methods, Vol. 370, Issue 1-2, pp. 93-103, (2011) (PubMed).

  • Target See all CCL1 ELISA Kits
    CCL1 (Chemokine (C-C Motif) Ligand 1 (CCL1))
    Alternative Name
    I-309 (CCL1 Products)
    Synonyms
    I-309 ELISA Kit, P500 ELISA Kit, SCYA1 ELISA Kit, SISe ELISA Kit, TCA3 ELISA Kit, BF534335 ELISA Kit, Scya1 ELISA Kit, Tca-3 ELISA Kit, CCLi6 ELISA Kit, chemokine ELISA Kit, C-C motif chemokine ligand 1 ELISA Kit, chemokine (C-C motif) ligand 1 ELISA Kit, CCL1 ELISA Kit, Ccl1 ELISA Kit
    Background
    Gene Names: CCL1 SCYA1
    Protein names: C-C motif chemokine 1 (Small-inducible cytokine A1) (T lymphocyte-secreted protein I-309)
    Gene ID
    6346
    UniProt
    P22362
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