Granzyme A (Granzyme 1, Cytotoxic T-Lymphocyte-Associated serine Esterase 3) (GZMA) ELISA Kit

Antigen: Granzyme A (Granzyme 1, Cytotoxic T-Lymphocyte-Associated serine Esterase 3) (GZMA)

Synonyms: HFSP, CTLA3, Hf, SE1, TSP1, Ctla3, TSP-1, Ctla-3, AW494114, gzmA, GZMA, MGC157236, MET1, LMET1

Application: ELISA

Catalog no.: ABIN629554

Additional Information

Alternative name: Granzyme A

Description: The human GRANZYME A ELISA kit is a sandwich-type of enzyme immunoassay in which a monoclonal GRANZYME A antibody is bound onto polystyrene microtiter wells. Human GRANZYME A, present in a measured volume of sample or standard is captured by the antibody on the microtiter well, and non-bound material is removed by washing. Subsequently, a biotinylated second monoclonal antibody to huGRANZYME A is added. This antibody binds to the huGRANZYME A-antibody complex present in the microtiter well. Excess biotinylated antibody is removed by washing, followed by the addition of horseradish peroxidase (HRP)-conjugated streptavidin, which binds to the biotinylated side of the huGRANZYME A sandwich. After removal of non-bound HRP conjugate by washing, a substrate solution is added to the wells. A coloured product is formed in proportion to the amount of huGRANZYME A present in the sample or standard. After the reaction has been terminated by the addition of a stop solution, absorbance is measured in a microtiter plate reader. From the absorbance of samples and those of a standard curve, the concentration of huGRANZYME A can be determined by interpolation with the standard curve.

Application Details

Storage: Store at -20 °C. Avoid repeated freeze/thaw cycles.

Research Area: Immunology, Innate Immunity, Adaptive Immunity, Apoptosis/Necrosis, Cell/Tissue Markers

Restrictions: For Research Use only