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E-cadherin ELISA Kit

CDH1 Reactivity: Mouse AA 157-709 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (heparin), Serum
Catalog No. ABIN921078
  • Target See all E-cadherin (CDH1) ELISA Kits
    E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))
    Binding Specificity
    AA 157-709
    Reactivity
    • 5
    • 4
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Mouse
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    156-10000 pg/mL
    Minimum Detection Limit
    156 pg/mL
    Application
    ELISA
    Purpose
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse E-Cadherin
    Brand
    PicoKine™
    Sample Type
    Cell Culture Supernatant, Serum, Plasma (heparin)
    Analytical Method
    Quantitative
    Specificity
    Expression system for standard: NSO
    Immunogen sequence: D157-V709
    Cross-Reactivity (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivity
    <15pg/mL
    Material not included
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: NSO
    Immunogen sequence: D157-V709
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  • Application Notes
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Comment

    Tissue Specificity: Non-neural epithelial tissues.

    Plate
    Pre-coated
    Protocol
    mouse E-Cadherin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for E-Cadherin has been precoated onto 96-well plates. Standards(NSO, D157-V709) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for E-Cadherin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse E-Cadherin amount of sample captured in plate.
    Assay Procedure

    Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 313pg/mL, 156pg/mL mouse E-Cadherin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse E-Cadherin standard solution and each sample is measured in duplicate.

    Assay Precision
    • Sample 1: n=16, Mean(ng/ml): 1.09, Standard deviation: 0.062, CV(%): 5.7
    • Sample 2: n=16, Mean(ng/ml): 3.31, Standard deviation: 0.209, CV(%): 6.3
    • Sample 3: n=16, Mean(ng/ml): 6.76, Standard deviation: 0.487, CV(%): 7.2,
    • Sample 1: n=24, Mean(ng/ml): 1.14, Standard deviation: 0.072, CV(%): 6.3
    • Sample 2: n=24, Mean(ng/ml): 3.59, Standard deviation: 0.255, CV(%): 7.1
    • Sample 3: n=24, Mean(ng/ml): 7.23, Standard deviation: 0.528, CV(%): 7.3
    Restrictions
    For Research Use only
  • Handling Advice
    Avoid multiple freeze-thaw cycles.
    Storage
    -20 °C,4 °C
    Storage Comment
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Expiry Date
    12 months
  • Target See all E-cadherin (CDH1) ELISA Kits
    E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))
    Alternative Name
    CDH1 (CDH1 Products)
    Synonyms
    Arc-1 ELISA Kit, CD324 ELISA Kit, CDHE ELISA Kit, ECAD ELISA Kit, LCAM ELISA Kit, UVO ELISA Kit, E-cadherin ELISA Kit, cdh1 ELISA Kit, si:dz180o5.2 ELISA Kit, uvo ELISA Kit, lcam ELISA Kit, E-Cad ELISA Kit, XBcad ELISA Kit, l-cam ELISA Kit, xcdh1 ELISA Kit, cdhc-A ELISA Kit, xb-cad ELISA Kit, XTCAD-1 ELISA Kit, c-cadherin ELISA Kit, cadherin-1 ELISA Kit, uvomorulin ELISA Kit, XB-cadherin ELISA Kit, arc-1 ELISA Kit, cd324 ELISA Kit, cdhe ELISA Kit, ecad ELISA Kit, AA960649 ELISA Kit, E-cad ELISA Kit, Ecad ELISA Kit, L-CAM ELISA Kit, Um ELISA Kit, cadherin 1 ELISA Kit, si:busm1-180o5.2 ELISA Kit, cadherin 1, type 1, E-cadherin (epithelial) ELISA Kit, cadherin 1, type 1 S homeolog ELISA Kit, CDH1 ELISA Kit, si:busm1-180o5.2 ELISA Kit, cdh1 ELISA Kit, Cdh1 ELISA Kit, cdh1.S ELISA Kit
    Background

    Protein Function: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells, cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7 (By similarity). .

    Background: E-Cadherin, also called Cadherin 1(CDH1), Uvomorulin or calcium-dependent ashesion protein, epithelial. E-cadherin is a Ca(2+)-dependent epithelial cell-cell adhesion molecule. Downregulation of E-cadherin expression often correlates with strong invasive potential and poor prognosis of human carcinomas. The gene spans a region of approximately 100 kb, and its location on chromosome 16q22.1. It contains 16 exons and a 65-kb-long intron 2. E-cadherin gene mutations may contribute to the development of diffusely growing gastric carcinomas. E-cadherin plays a central part in the process of epithelial morphogenesis and acts as a strong invasion suppressor in experimental tumor cell systems.

    Synonyms: Cadherin-1,ARC-1,Epithelial cadherin,E-cadherin,Uvomorulin,CD324,E-Cad/CTF1,E-Cad/CTF2,E-Cad/CTF3,Cdh1,

    Full Gene Name: Cadherin-1

    Cellular Localisation: Cell junction. Cell membrane, Single-pass type I membrane protein. Endosome . Golgi apparatus, trans-Golgi network . Colocalizes with DLGAP5 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma-catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Colocalizes with RAB11A endosomes during its transport from the Golgi apparatus to the plasma membrane (By similarity)..
    Gene ID
    12550
    UniProt
    P09803
    Pathways
    WNT Signaling, Sensory Perception of Sound, Cell-Cell Junction Organization, Tube Formation
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