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CD56 ELISA Kit

NCAM1 Reactivity: Human AA 20-718 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Catalog No. ABIN921091
  • Target See all CD56 (NCAM1) ELISA Kits
    CD56 (NCAM1) (Neural Cell Adhesion Molecule 1 (NCAM1))
    Binding Specificity
    AA 20-718
    Reactivity
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    156-10000 pg/mL
    Minimum Detection Limit
    156 pg/mL
    Application
    ELISA
    Purpose
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CD56/NCAM-1
    Brand
    PicoKine™
    Sample Type
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    Specificity
    Expression system for standard: NSO
    Immunogen sequence: L20-G718
    Cross-Reactivity (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivity
    <10pg/mL
    Material not included
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: NSO
    Immunogen sequence: L20-G718
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  • Application Notes
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Comment

    Sequence similarities: Contains 2 fibronectin type-III domains.

    Plate
    Pre-coated
    Protocol
    human CD56 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD56 has been precoated onto 96-well plates. Standards(NSO, L20-G718) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD56 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD56 amount of sample captured in plate.
    Assay Procedure

    Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human CD56 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human CD56 standard solution and each sample is measured in duplicate.

    Assay Precision
    • Sample 1: n=16, Mean(ng/ml): 1.52, Standard deviation: 0.082, CV(%): 5.4
    • Sample 2: n=16, Mean(ng/ml): 3.48, Standard deviation: 0.212, CV(%): 6.1
    • Sample 3: n=16, Mean(ng/ml): 5.34, Standard deviation: 0.368, CV(%): 6.9,
    • Sample 1: n=24, Mean(ng/ml): 2.06, Standard deviation: 0.140, CV(%): 6.8
    • Sample 2: n=24, Mean(ng/ml): 4.16, Standard deviation: 0.312, CV(%): 7.5
    • Sample 3: n=24, Mean(ng/ml): 6.27, Standard deviation: 0.508, CV(%): 8.1
    Restrictions
    For Research Use only
  • Handling Advice
    Avoid multiple freeze-thaw cycles.
    Storage
    -20 °C,4 °C
    Storage Comment
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Expiry Date
    12 months
  • Target See all CD56 (NCAM1) ELISA Kits
    CD56 (NCAM1) (Neural Cell Adhesion Molecule 1 (NCAM1))
    Alternative Name
    NCAM1 (NCAM1 Products)
    Synonyms
    CD56 ELISA Kit, MSK39 ELISA Kit, NCAM ELISA Kit, fj56g09 ELISA Kit, ncam ELISA Kit, ncam1 ELISA Kit, wu:fj56g09 ELISA Kit, NCAM1 ELISA Kit, n-cam ELISA Kit, E-NCAM ELISA Kit, NCAM-1 ELISA Kit, Ncam ELISA Kit, Cd56 ELISA Kit, N-CAM ELISA Kit, N-CAM-1 ELISA Kit, NCAM-C ELISA Kit, NCAMC ELISA Kit, N-CAM-1-B ELISA Kit, NCAM-1-B ELISA Kit, ncam1-a ELISA Kit, ncam1-b ELISA Kit, neural cell adhesion molecule 1 ELISA Kit, neural cell adhesion molecule 1a ELISA Kit, neural cell adhesion molecule 1 S homeolog ELISA Kit, neural cell adhesion molecule 1 L homeolog ELISA Kit, NCAM1 ELISA Kit, ncam1a ELISA Kit, ncam1 ELISA Kit, LOC100085373 ELISA Kit, ncam1.S ELISA Kit, Ncam1 ELISA Kit, ncam1.L ELISA Kit
    Background

    Protein Function: This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.

    Background: Neural Cell Adhesion Molecule, NCAM, also known as the cluster of differentiation CD56, is a hemophilic binding glycoprotein. It is a glycoprotein of Immunoglobulin(Ig) super family. At least 27 alternatively spliced NCAM mRNAs are produced, giving a wide diversity of NCAM isoforms NCAM gene is located at 11q22-q23. This glycoprotein is mainly expressed on the surface of neurons, glia, skeletal muscle and natural killer cells. NCAM has been implicated as having a role in cellâ€"cell adhesion, neurite outgrowth, synaptic plasticity, and learning and memory.

    Synonyms: Neural cell adhesion molecule 1,N-CAM-1,NCAM-1,CD56,NCAM1,NCAM,

    Full Gene Name: Neural cell adhesion molecule 1

    Cellular Localisation: Isoform 1: Cell membrane, Single-pass type I membrane protein.
    Gene ID
    4684
    UniProt
    P13591
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