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CEACAM5 ELISA Kit

CEACAM5 Reactivity: Human AA 35-685 Colorimetric Sandwich ELISA 312-20000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Catalog No. ABIN921121
  • Target See all CEACAM5 ELISA Kits
    CEACAM5 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5 (CEACAM5))
    Binding Specificity
    AA 35-685
    Reactivity
    • 2
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    312-20000 pg/mL
    Minimum Detection Limit
    312 pg/mL
    Application
    ELISA
    Purpose
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CEA
    Brand
    PicoKine™
    Sample Type
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    Specificity
    Expression system for standard: NSO
    Immunogen sequence: K35-A685
    Cross-Reactivity (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivity
    <10pg/mL
    Material not included
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: NSO
    Immunogen sequence: K35-A685
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  • Application Notes
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Comment

    Sequence similarities: Belongs to the immunoglobulin superfamily. CEA family.

    Tissue Specificity: Found in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon.

    Plate
    Pre-coated
    Protocol
    human CEA ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CEA has been precoated onto 96-well plates. Standards(NSO, K35-A685) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CEA is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CEA amount of sample captured in plate.
    Assay Procedure

    Aliquot 0.1 mL per well of the 20000pg/mL, 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human CEA standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma( heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human CEA standard solution and each sample be measured in duplicate.

    Assay Precision
    • Sample 1: n=16, Mean(ng/ml): 2.36, Standard deviation: 0.113, CV(%): 4.8
    • Sample 2: n=16, Mean(ng/ml): 5.59, Standard deviation: 0.235, CV(%): 4.2
    • Sample 3: n=16, Mean(ng/ml): 10.4, Standard deviation: 0.53, CV(%): 5.1,
    • Sample 1: n=24, Mean(ng/ml): 2.48, Standard deviation: 0.171, CV(%): 6.9
    • Sample 2: n=24, Mean(ng/ml): 5.56, Standard deviation: 0.395, CV(%): 7.1
    • Sample 3: n=24, Mean(ng/ml): 10.7, Standard deviation: 0.556, CV(%): 5.2
    Restrictions
    For Research Use only
  • Handling Advice
    Avoid multiple freeze-thaw cycles.
    Storage
    -20 °C,4 °C
    Storage Comment
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Expiry Date
    12 months
  • Hu, Zheng, Su, Wang, Wu, Hu: "Ultrasensitive all-carbon photoelectrochemical bioprobes for zeptomole immunosensing of tumor markers by an inexpensive visible laser light." in: Analytical chemistry, Vol. 85, Issue 21, pp. 10612-9, (2013) (PubMed).

  • Target See all CEACAM5 ELISA Kits
    CEACAM5 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5 (CEACAM5))
    Alternative Name
    CEACAM5 (CEACAM5 Products)
    Synonyms
    CD66e ELISA Kit, CEA ELISA Kit, CEACAM5 ELISA Kit, CEACAM ELISA Kit, 1600029H12Rik ELISA Kit, Psg30 ELISA Kit, carcinoembryonic antigen related cell adhesion molecule 5 ELISA Kit, carcinoembryonic antigen ELISA Kit, carcinoembryonic antigen-related cell adhesion molecule 5 ELISA Kit, carcinoembryonic antigen-related cell adhesion molecule 8 ELISA Kit, carcinoembryonic antigen gene family ELISA Kit, CEACAM5 ELISA Kit, Ceacam5 ELISA Kit, LOC100409241 ELISA Kit, Cea ELISA Kit
    Background

    Protein Function: Cell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. Receptor for E.coli Dr adhesins. .

    Background: Carcinoembryonic antigen(CEA) is a complex immunoreactive glycoprotein with a molecular weight of 180,000 comprising 60 % carbohydrate. It is found in adenocarcinomas of endodermally derived digestive system epithelia and in fetal colon.Carcinoembryonic antigen is one of the most widely used tumor markers in serum immunoassay determinations of carcinoma.

    Synonyms: Carcinoembryonic antigen-related cell adhesion molecule 5,Carcinoembryonic antigen,CEA,Meconium antigen 100,CD66e,CEACAM5,CEA,

    Full Gene Name: Carcinoembryonic antigen-related cell adhesion molecule 5

    Cellular Localisation: Cell membrane, Lipid-anchor, GPI-anchor.
    Gene ID
    111518
    UniProt
    P06731
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