LPO-CC Kit
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- Target
- LPO-CC
- Detection Method
- Colorimetric
- Detection Range
- 2.0-300 nM/mL
- Minimum Detection Limit
- 2 nM/mL
- Sample Type
- Plasma, Serum
- Sensitivity
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2.0 nmol/mL
When a blank is used as a sample, the absorbance should be below 0.100. When a Calibrator containing 50 nmol/mL of cumene peroxides is assayed, the absorbance should be within the range of 0.115-0.135 after subtracting the blank. - Components
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Reagent 1: Enzyme Reagent: 1 x 55 mL bottle of Ascorbic Oxidase, Lipoprotein Lipase, Stabilizer, Goods Buffer, Surfactants
Reagent 2A: Lyophilized Chromogen Reagent: 1 x bottle of MCDP (for 110 mL)
Reagent 2B: Buffer Solution (liquid): 1 x 110 mL bottle of Goods Buffer, Hemoglobin, Surfactants Calibrator (liquid): (1 x 6 mL)
bottle of 50 nmol/mL Cumene Hydroperoxide
CAUTION: Store all components in the dark. Reagents are light sensitive. - Material not included
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Spectrophotometer: capable of accurate absorbance reading at 675 nm with appropriate cuvettes.
Pipettes: capable of dispensing accurate volumes.
Test Tubes: glass or plastic.
Water Bath: capable of maintaining 30° C.
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- Reagent Preparation
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Reagent 1 (R-1): Ready to use.
Reagent 2 (R-2): Dissolve all of Reagent 2A in Reagent 2B to make Reagent
2. Dissolved reagent should be protected from light at all times by wrapping in aluminum foil. Transfer all of the dissolved reagent back to the large Reagent 2B bottle and protect from light with aluminum foil. Incubate for 15 min. at 37°C after dissolving. - Sample Preparation
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Serum or plasma should be separated immediately from blood samples. The assay should be run within a few hours. Samples can be stored but not for longer than 2 weeks under any storage conditions. The LPO-CC kit can be used on biological samples other than serum and plasma. However, an initial sample extraction step may be required (see below). Suitable for two-reagent automated analyzers. Refer to the instrument manufacturer's manual for the following: a) Use or function. b) Installation procedures and requirements. c) Principles of operation. d) Performance characteristics and specifications. e) Operation instructions. f) Calibration procedures including materials and/or equipment to be used. g) Operational precautions and limitations. h) Hazards. i) Service and maintenance.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Storage
- 4 °C
- Storage Comment
- Store all unopened kit components at 4° C. Unopened reagents stored at 4° C and protected from light are stable until the expiration date on the label. Dissolved (reconstituted) reagent is stable for 3 days when stored in the dark at 4° C and protected from light by wrapping the bottle in foil. If aliquoted, wrap vials in foil and store frozen in the dark at -20° C for 2-3 months. Do not refreeze aliquoted reagents.
- Expiry Date
- The expiry date is stated on the label.
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- Target
- LPO-CC
- Background
- Lipid peroxides in serum are produced by auto-oxidation of unsaturated fatty acids in the human body. The quantitative determination of lipid peroxides is believed to be useful for research of various diseases. Lipid peroxides are very unstable in the body and are present in very low levels. Therefore, it is necessary to directly determine lipid peroxide levels by a quick and simple assay such as the LPO-CC.In the presence of hemoglobin, lipid hydroperoxides are reduced to hydroxyl derivatives (lipid alcohols) and the MCDP chromogen is oxidatively cleaved to form methylene blue in an equal molar reaction. Lipid peroxides are quantitated by colorimetrically measuring the methylene blue at 675 nm.
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