ENO2/NSE ELISA Kit

Catalog No. ABIN996903

Product Details ENO2/NSE ELISA Kit

Target: ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0-200 ng/mL

Minimum Detection Limit: 0 ng/mL

Application: ELISA

Purpose: The Neuron-Specific Enolase (NSE) enzyme linked immunosorbent assay (ELISA) provides quantitative measurement of human NSE in serum to aid in the clinical evaluation of patients suspected of having small cell lung cancer, and other related diseases.

Sample Type: Serum

Analytical Method: Quantitative

Specificity: 98.7 %

Sensitivity: 15 ng/mL

Application Details

Sample Volume: 15 μL

Assay Time: 1 - 2 h

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12-14 months

Target Details for ENO2/NSE

Target: ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))

Alternative Name: NSE (ENO2 Products)

Synonyms: ENO2 ELISA Kit, DKFZp459B1817 ELISA Kit, NSE ELISA Kit, AI837106 ELISA Kit, D6Ertd375e ELISA Kit, Eno-2 ELISA Kit, RNEN3 ELISA Kit, eno3 ELISA Kit, wu:fc09h05 ELISA Kit, zgc:92418 ELISA Kit, enolase 2 ELISA Kit, enolase 2 (gamma, neuronal) ELISA Kit, enolase 2, gamma neuronal ELISA Kit, enolase 2, gamma, neuronal ELISA Kit, ENO2 ELISA Kit, Eno2 ELISA Kit, eno2 ELISA Kit

Background: The glycolytic enzyme enolase (2-phosph-D-glycerate hydrolyase) exists as several dimeric isoenzymes ( alphaalpha , alphabeta alphagamma and gammagamma ) composed of three distinct subunits, alpha , beta , and gamma . Three isoenzymes are found in human brain: alphaalpha , alphagamma and gammagamma . The alphagamma and gammagamma -enolase isoenzymes are also known as neuron-specific enolase (NSE) as these isoenzymes initially were detected in neurons and neuronendocrine cells. The NSE levels are low in health and benign subjects. Elevated levels are commonly found in patients with malignant tumors with neuronendocrine differentiation, especially small cell lung cancer and neuroblastoma. Lung cancer is one of the most spread cancer forms with incidences about 50~100 per 100,000 population.
Approximately 20% of the lung cancer is small cell lung cancer. Patients with small cell lung cancer show various proportions of alphagamma and gammagamma isoenzyme. The determination of NSE should detect and gamma isoforms with the same sensitivity. The antibodies for this particular assay are specific for the gamma -subunit without cross reactivity with alpha or beta subunits. NSE Kits are reported to be useful diagnostic marker for lung cancer, neuroblastoma, and melanoma, seminoma (S) and in injury of central nervous system. In addition to the above, NSE can be a valuable tool in following-up the effect of chemotherapy of small cell lung cancer, in prognostic evaluation of patients with small cell lung cancer, and in differential diagnosis between cell lung cancer and non-small cell lung cancer.