Competitive ELISA

In a competitive format assay, as antigen concentration in a sample increases, signal intensity decreases.The central concept behind a competitive ELISA is that a larger quantity of analyte in a sample results in fewer free antibodies in solution and by extension a smaller number of labeled antibodies bound to the standard on the plate.

A primary antibody (unlabeled) is incubated with sample antigen. They are then added to 96-well plates which are pre-coated with the same antigen, followed by a washing step. Antigen quantity in the sample determines the amount antibody binding on the well. The secondary antibody that is specific to the primary antibody and conjugated with an enzyme is added. A substrate is added, and remaining enzymes elicit a chromogenic or fluorescent signal. The competitive assay is an appealing option if no suitable antibody pair can be identified for sandwich ELISA, or if the analyte in question is too small to permit binding of both a capture and detection antibody. Our support team will gladly help you with finding the right ELISA kit for your needs. Please contact us via phone, email or use our live chat system.

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28,957 Products
ReactivityMouse (Murine)
Detection Minimum49.4 pg/mL
Order details Cat. No. ABIN415519
$545.68
Plus shipping costs $45.00 Will be delivered in 13 to 16 Business Days
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ReactivityHuman
Detection Minimum0.025 μg/mL
Order details Cat. No. ABIN365026
» Show selected B2M ELISA Kits with more information such as references or interaction partners.
$633.11
Plus shipping costs $45.00 Will be delivered in 13 to 16 Business Days
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ReactivityHuman
Detection Minimum0.312 ng/mL
Order details Cat. No. ABIN1690717
$666.11
Plus shipping costs $45.00 Will be delivered in 13 to 16 Business Days
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ReactivityChemical
Detection Minimum1 ng/mL
Order details Cat. No. ABIN1721161
$605.00
96 tests
Plus shipping costs $45.00 Will be delivered in 1 to 2 Business Days
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ReactivityHuman
Detection Minimum50 μg/mL
Order details Cat. No. ABIN510001
$707.14
96 tests
Plus shipping costs $45.00 Will be delivered in 15 to 18 Business Days
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ReactivityHuman, Mouse (Murine), Rat (Rattus)
Detection Minimum0.1 pg/mL
Order details Cat. No. ABIN1979095
» Show selected NPPB ELISA Kits with more information such as references or interaction partners.
$452.10
Plus shipping costs $45.00 Will be delivered in 2 to 3 Business Days
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ReactivityHuman
Detection Minimum2.4 pg/mL
Order details Cat. No. ABIN2506977
» Show selected AVP ELISA Kits with more information such as references or interaction partners.
$411.40
96 tests
Plus shipping costs $45.00 Will be delivered in 3 to 4 Business Days
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ReactivityMouse (Murine)
Detection Minimum0.024 ng/mL
Order details Cat. No. ABIN2506978
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$411.40
96 tests
Plus shipping costs $45.00 Will be delivered in 3 to 4 Business Days
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ReactivityMouse (Murine)
Detection Minimum2.4 pg/mL
Order details Cat. No. ABIN2506979
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$411.40
96 tests
Plus shipping costs $45.00 Will be delivered in 3 to 4 Business Days
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ReactivityRat (Rattus)
Detection Minimum0.024 ng/mL
Order details Cat. No. ABIN2506982
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$411.40
96 tests
Plus shipping costs $45.00 Will be delivered in 3 to 4 Business Days
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ReactivityRat (Rattus)
Detection Minimum2.4 pg/mL
Order details Cat. No. ABIN2506985
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$411.40
96 tests
Plus shipping costs $45.00 Will be delivered in 3 to 4 Business Days
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ReactivityRat (Rattus)
Detection Minimum2.4 pg/mL
Order details Cat. No. ABIN2506983
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$411.40
96 tests
Plus shipping costs $45.00 Will be delivered in 3 to 4 Business Days
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ReactivityCow (Bovine)
Detection Minimum0.024 ng/mL
Order details Cat. No. ABIN2506986
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$411.40
96 tests
Plus shipping costs $45.00 Will be delivered in 3 to 4 Business Days
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ReactivityCow (Bovine)
Detection Minimum2.4 pg/mL
Order details Cat. No. ABIN2506987
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$411.40
96 tests
Plus shipping costs $45.00 Will be delivered in 3 to 4 Business Days
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ReactivityRat (Rattus)
Detection Minimum9.8 pg/mL
Order details Cat. No. ABIN2506984
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$411.40
96 tests
Plus shipping costs $45.00 Will be delivered in 3 to 4 Business Days
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ReactivityCow (Bovine)
Detection Minimum9.8 pg/mL
Order details Cat. No. ABIN2506988
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$411.40
96 tests
Plus shipping costs $45.00 Will be delivered in 3 to 4 Business Days
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ReactivityCow (Bovine)
Detection Minimum2.4 pg/mL
Order details Cat. No. ABIN2506989
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$411.40
96 tests
Plus shipping costs $45.00 Will be delivered in 3 to 4 Business Days
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ReactivityHuman
Detection Minimum2.4 pg/mL
Order details Cat. No. ABIN2506975
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$411.40
96 tests
Plus shipping costs $45.00 Will be delivered in 3 to 4 Business Days
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ReactivityHuman
Detection Minimum0.024 ng/mL
Order details Cat. No. ABIN2506974
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$411.40
96 tests
Plus shipping costs $45.00 Will be delivered in 3 to 4 Business Days
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ReactivityHuman
Detection Minimum9.8 pg/mL
Order details Cat. No. ABIN2506976
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$411.40
96 tests
Plus shipping costs $45.00 Will be delivered in 3 to 4 Business Days
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ReactivityMouse (Murine)
Detection Minimum9.8 pg/mL
Order details Cat. No. ABIN2506980
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$411.40
96 tests
Plus shipping costs $45.00 Will be delivered in 3 to 4 Business Days
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ReactivityMouse (Murine)
Detection Minimum2.4 pg/mL
Order details Cat. No. ABIN2506981
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$411.40
96 tests
Plus shipping costs $45.00 Will be delivered in 3 to 4 Business Days
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ReactivityMouse (Murine)
Detection Minimum12.35 pg/mL
Order details Cat. No. ABIN415632
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$505.26
Plus shipping costs $45.00 Will be delivered in 13 to 16 Business Days
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ReactivityHuman
Detection Minimum24.69 pg/mL
Order details Cat. No. ABIN1873353
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$530.53
Plus shipping costs $45.00 Will be delivered in 13 to 16 Business Days
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ReactivityHuman
Detection Minimum123.5 pg/mL
Order details Cat. No. ABIN414671
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$530.53
Plus shipping costs $45.00 Will be delivered in 13 to 16 Business Days
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ReactivityHuman
Detection Minimum12.35 pg/mL
Order details Cat. No. ABIN415019
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$545.68
Plus shipping costs $45.00 Will be delivered in 13 to 16 Business Days
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ReactivityCow (Bovine)
Detection Minimum5 pg/mL
Order details Cat. No. ABIN364641
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$812.78
Plus shipping costs $45.00 Will be delivered in 13 to 16 Business Days
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ReactivityCow (Bovine)
Detection Minimum62.5 pg/mL
Order details Cat. No. ABIN456866
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$812.78
Plus shipping costs $45.00 Will be delivered in 13 to 16 Business Days
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ReactivityHuman
Detection Minimum0.1 μg/mL
Order details Cat. No. ABIN457028
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$910.56
Plus shipping costs $45.00 Will be delivered in 13 to 16 Business Days
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ReactivityHuman
Detection Minimum1.56 μg/mL
Order details Cat. No. ABIN366135
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$910.56
Plus shipping costs $45.00 Will be delivered in 13 to 16 Business Days
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