TIMP2 293 Cell Lysate

Details for Product No. ABIN1602923, Supplier: Log in to see
Protein
  • CSC-21K
  • DDC8
  • TIMP-2
  • D11Bwg1104e
  • Timp-2
  • etID32613.12
  • fa96h09
  • timp2
  • wu:fa96h09
  • zgc:136726
  • TIMP metallopeptidase inhibitor 2
  • tissue inhibitor of metalloproteinase 2
  • TIMP metallopeptidase inhibitor 2a
  • TIMP2
  • Timp2
  • timp2a
Protein Species
Human
Conjugate
Myc-DYKDDDDK Tag
Application
ELISA, Immunoprecipitation (IP), Western Blotting (WB)
Options
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Brand TACL™
Characteristics Antigen standard for TIMP metallopeptidase inhibitor 2 (TIMP2) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid (True ORF cDNA clone RC209796, OriGene Technologies, Inc.) and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Components This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer.
Lysate Fraction Whole Cell Lysate
Lysate Type Cell Lysate
Lysed Cells HEK 293T Cells
Alternative Name TIMP2
Background Alternate Names: tissue inhibitor of metalloproteinase 2, tissue inhibitor of metalloproteinases 2, TIMP-2, metalloproteinase inhibitor 2, TIMP2, CSC-21K
NCBI Accession NP_003246
Research Area Cardiovascular, Atherosclerosis, Proteolysis / Ubiquitin, Angiogenesis, Extracellular Matrix, Matrix Metalloproteinases, Cancer, Inflammation
Application Notes WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 μg lysate per lane.
Restrictions For Research Use only
Format Liquid
Buffer Each lysate vial contains 0.1 mg lysate in 0.1 mL (1 mg/mL) of RIPA Buffer (50 mM Tris-HCl pH 7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1 % NP40). The loading buffer vial contains 0.5 mL 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10 % glycerol, 4 % SDS, 0.002 % Bromophenol blue, 5 % beta-mercaptoethanol).
Handling Advice Minimize freeze-thaw cycles.
Storage -20 °C,-80 °C
Storage Comment Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Expiry Date 6 months