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anti-Human ARAF antibody for Enzyme Immunoassay

Recommended ARAF Antibody (supplied by: Log in to see )

Antigen
V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) Antibodies
  • 1200013E08Rik
  • A-RAF
  • a-raf
  • A-Raf
  • araf
  • ARAF
  • ARAF1
  • araf1
  • Araf1
  • AW495444
  • pks2
  • PKS2
  • rafa1
  • RAFA1
  • wu:fk45h07
  • zgc:92074
Epitope
Middle Region
21
20
18
15
14
13
13
9
8
5
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
127
67
62
3
3
2
2
1
1
Host
Rabbit
107
22
Clonality
Polyclonal
Conjugate
This ARAF antibody is un-conjugated
5
5
5
3
3
3
3
3
3
3
3
3
2
2
2
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
85
52
33
27
18
10
4
3
2
1
1
1
1
Supplier
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Catalog No. ABIN360127
$ 390.50
Plus shipping costs $45.00

Top referenced anti-ARAF antibody for Enzyme Immunoassay

Similar anti-ARAF Antibodies

Application / Reactivity Human
ELISA 51 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Immunocytochemistry (ICC) 5 Antibodies
Immunofluorescence (IF) 11 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 33 Antibodies
Immunohistochemistry (IHC) 29 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 21 Antibodies
Immunoprecipitation (IP) 3 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Western Blotting (WB) 86 Antibodies

General

Antigen V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) Antibodies
Epitope Middle Region
(21), (20), (18), (15), (14), (13), (13), (9), (8), (5), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(127), (67), (62), (3), (3), (2), (2), (1), (1)
Host Rabbit
(107), (22)
Clonality
Polyclonal
Conjugate This ARAF antibody is un-conjugated
(5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(85), (52), (33), (27), (18), (10), (4), (3), (2), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-ARAF Antibody

Target Details ARAF Application Details Handling References for anti-ARAF Antibody (ABIN360127) Images
Specificity This antibody reacts to A-RAF.
Purification Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human ARAF1.
Isotype Ig

Target Details ARAF

Product Details anti-ARAF Antibody Application Details Handling References for anti-ARAF Antibody (ABIN360127) Images back to top
Antigen
Alternative Name ARAF (ARAF Antibody Abstract)
Background Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.Synonyms: A-Raf proto-oncogene serine/threonine-protein kinase, A-raf-1, ARAF1, PKS, PKS2, Proto-oncogene Pks
Gene ID 369, 9606
UniProt P10398
Pathways MAPK Signaling, RTK Signaling

Application Details

Product Details anti-ARAF Antibody Target Details ARAF Handling References for anti-ARAF Antibody (ABIN360127) Images back to top
Application Notes ELISA: 1/1,000. Immunohistochemistry: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-ARAF Antibody Target Details ARAF Application Details References for anti-ARAF Antibody (ABIN360127) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-ARAF Antibody (ABIN360127)

Product Details anti-ARAF Antibody Target Details ARAF Application Details Handling Images back to top
Background publications

Wu, Noh, Zhou, Dixon, Guan: "Selective activation of MEK1 but not MEK2 by A-Raf from epidermal growth factor-stimulated Hela cells." in: The Journal of biological chemistry, Vol. 271, Issue 6, pp. 3265-71, 1996

Lee, Beck, Brennscheidt, DeGennaro, Rapp: "The complete sequence and promoter activity of the human A-raf-1 gene (ARAF1)." in: Genomics, Vol. 20, Issue 1, pp. 43-55, 1994

Popescu, Mark: "Localization of the pKs gene, a raf related sequence on human chromosomes X and 7." in: Oncogene, Vol. 4, Issue 4, pp. 517-9, 1989

Beck, Huleihel, Gunnell, Bonner, Rapp: "The complete coding sequence of the human A-raf-1 oncogene and transforming activity of a human A-raf carrying retrovirus." in: Nucleic acids research, Vol. 15, Issue 2, pp. 595-609, 1987

Images

Product Details anti-ARAF Antibody Target Details ARAF Application Details Handling References for anti-ARAF Antibody (ABIN360127) back to top
Supplier Images
 image for anti-ARAF antibody (V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog) (Middle Region) (ABIN360127) anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (Middle Region) antibody