anti-Mouse (Murine) ARAF antibody for Western Blotting

Recommended ARAF Antibody (supplied by: Log in to see )

Antigen
V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) Antibodies
  • zgc:92074
  • wu:fk45h07
  • ARAF
  • a-raf
  • araf
  • araf1
  • pks2
  • rafa1
  • A-RAF
  • ARAF1
  • PKS2
  • RAFA1
  • 1200013E08Rik
  • A-Raf
  • AW495444
  • Araf1
  • v-raf murine sarcoma 3611 viral oncogene homolog
  • araf
  • ARAF
  • LOC100340437
  • araf-a
  • araf-b
  • Araf
Epitope
Middle Region
23
20
16
15
14
12
12
10
8
5
4
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine)
146
81
74
5
5
5
2
2
1
1
1
1
Host
Rabbit
120
26
Clonality
Polyclonal
Conjugate
This ARAF antibody is un-conjugated
5
5
5
3
3
3
3
3
3
3
3
3
3
3
2
2
2
Application
Immunohistochemistry (IHC), Western Blotting (WB)
98
52
39
29
28
14
6
3
2
2
1
1
1
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Catalog No. ABIN2785788
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
2.906104 ABIN2705360 WB Rabbit pTyr302 Log in to see Polyclonal
2.906104 ABIN967727 IF IP IHC WB Mouse IgG1 AA 183-199 Log in to see 1-A 5
2.906104 ABIN1531487 IF IHC ELISA WB Rabbit IgG pTyr302 Log in to see Polyclonal
2.906104 ABIN1532501 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
2.906104 ABIN3183344 ELISA IHC WB Rabbit IgG Ser155 Log in to see Polyclonal
2.906104 ABIN3183345 ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal
2.906104 ABIN2855039 ICC IF IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal
2.906104 ABIN4902938 WB Rabbit IgG Log in to see Polyclonal
2.906104 ABIN443011 ICC IF IHC (p) IHC WB Rabbit IgG C-Term Log in to see Polyclonal
2.906104 ABIN634291 WB Rabbit Middle Region Log in to see Polyclonal
1 ABIN322103 WB Rabbit IgG AA 254-303 Log in to see Polyclonal
1 ABIN1450815 ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal 1
1 ABIN408002 IHC (p) IHC WB Rabbit IgG Log in to see Polyclonal
1 ABIN2891222 IHC (p) ELISA WB Rabbit IgG AA 276-325 Log in to see Polyclonal
1 ABIN271994 IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN745328 IF (p) IHC (p) WB Rabbit IgG pSer299 Log in to see Polyclonal
1 ABIN746078 IF (p) IHC (p) WB Rabbit IgG pTyr301, pTyr302 Log in to see Polyclonal
1 ABIN3182781 ELISA IF IHC WB Rabbit IgG pTyr302 Log in to see Polyclonal
1 ABIN682777 IHC (p) WB HRP Rabbit IgG pSer214 Log in to see Polyclonal
1 ABIN745337 IHC (p) WB HRP Rabbit IgG pSer299 Log in to see Polyclonal

General

Antigen V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) Antibodies
Epitope Middle Region
(23), (20), (16), (15), (14), (12), (12), (10), (8), (5), (4), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine)
(146), (81), (74), (5), (5), (5), (2), (2), (1), (1), (1), (1)
Host Rabbit
(120), (26)
Clonality
Polyclonal
Conjugate This ARAF antibody is un-conjugated
(5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(98), (52), (39), (29), (28), (14), (6), (3), (2), (2), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-ARAF Antibody

Target Details ARAF Application Details Handling ProductDetails: References for anti-ARAF antibody (ABIN2785788) Images
Blocking Peptide Blocking peptide for this product available: ABIN973357
Sequence PRGSPSPASV SSGRKSPHSK SPAEQRERKS LADDKKKVKN LGYRDSGYYW
Predicted Reactivity Cow: 100%, Dog: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 93%, Rat: 100%, Sheep: 100%
Characteristics This is a rabbit polyclonal antibody against ARAF. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human ARAF

Target Details ARAF

Product Details anti-ARAF Antibody Application Details Handling ProductDetails: References for anti-ARAF antibody (ABIN2785788) Images back to top
Antigen
Alternative Name ARAF (ARAF Antibody Abstract)
Background ARAF belongs to the protein kinase superfamily, TKL Ser/Thr protein kinase family, RAF subfamily. It contains 1 phorbol-ester/DAG-type zinc finger, 1 protein kinase domain, and 1 RBD (Ras-binding) domain. ARAF is involved in the transduction of mitogenic signals from the cell membrane to the nucleus.
Alias Symbols: A-RAF, ARAF1, PKS2, RAFA1
Protein Interaction Partner: AGTRAP, KRAS, CDC37, MAP2K1, GNA12, FKBP5, UBC, RNF2, FBXO6, YWHAB, HIPK4, YWHAE, RRAS2, BAD, TEKT4P2, WNK1, NELFCD, DIDO1, TESK1, MAP2K2, MLH1, HSP90AA1, E2F6, BRAF, TIRAP, IRF7, IRAK2, YWHAG, MYO18A, CHD6, YWHAZ, PBK, NUDT14, KLHL12, COPS3, TIMM44, ASS1
Protein Size: 606
Molecular Weight 67 kDa
Gene ID 369
NCBI Accession NM_001654, NP_001645
UniProt P10398
Pathways MAPK Signaling, RTK Signaling, Hepatitis C

Application Details

Product Details anti-ARAF Antibody Target Details ARAF Handling ProductDetails: References for anti-ARAF antibody (ABIN2785788) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 606 AA

Restrictions For Research Use only

Handling

Product Details anti-ARAF Antibody Target Details ARAF Application Details ProductDetails: References for anti-ARAF antibody (ABIN2785788) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

ProductDetails: References for anti-ARAF antibody (ABIN2785788)

Product Details anti-ARAF Antibody Target Details ARAF Application Details Handling Images back to top
Background publications

Razzaque, Nishizawa, Komoike, Yagi, Furutani, Amo, Kamisago, Momma, Katayama, Nakagawa, Fujiwara, Matsushima, Mizuno, Tokuyama, Hirota, Muneuchi, Higashinakagawa, Matsuoka: "Germline gain-of-function mutations in RAF1 cause Noonan syndrome." in: Nature genetics, Vol. 39, Issue 8, pp. 1013-7, 2007

Images

Product Details anti-ARAF Antibody Target Details ARAF Application Details Handling ProductDetails: References for anti-ARAF antibody (ABIN2785788) back to top
Supplier Images
Western Blotting (WB) image for anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (Middle Region) antibody (ABIN2785788) WB Suggested Anti-ARAF Antibody Titration: 0.2-1 ug/ml
Positive Control:...