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|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|ELISA||70 Antibodies||67 Antibodies|
|Enzyme Immunoassay (EIA)||6 Antibodies||7 Antibodies|
|Immunocytochemistry (ICC)||5 Antibodies||3 Antibodies|
|Immunofluorescence (IF)||46 Antibodies||39 Antibodies|
|Immunofluorescence (fixed cells) (IF/ICC)||4 Antibodies||4 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||92 Antibodies||105 Antibodies|
|Immunohistochemistry (IHC)||170 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||4 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||82 Antibodies|
|Immunoprecipitation (IP)||80 Antibodies|
|Western Blotting (WB)||220 Antibodies|
|Antigen||Activating Transcription Factor 2 (ATF2) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This ATF2 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
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Product Details anti-ATF2 AntibodyTarget Details ATF2 Application Details Handling Images
|Specificity||This antibody detects endogenous levels of ATF2 protein (region surrounding Asp67/49)|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Purification||The antibody was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen|
|Purity||> 95 % pure by SDS-PAGE|
Target Details ATF2Product Details anti-ATF2 Antibody Application Details Handling Images back to top
|Alternative Name||ATF2 (ATF2 Antibody Abstract)|
|Background||The transcription factor ATF-2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins. ATF-2 interacts with a variety of viral oncoproteins and cellular tumor suppressors and is a target of the SAPK/JNK and p38 MAP kinase signaling pathways. Various forms of cellular stress, including genotoxic agents, inflammatory cytokines and UV irradiation, stimulate the transcriptional activity of ATF-2. Cellular stress activates ATF-2 by phosphorylation of Thr69 and Thr71. Both SAPK and p38 MAPK have been shown to phosphorylate ATF-2 at these sites in vitro and in cells transfected with ATF-2. Mutations of these sites result in the loss of stress-induced transcription by ATF-2. In addition, mutations at these sites reduce the ability of E1A and Rb to stimulate gene expression via ATF-2.Synonyms: ATF-2, Activating transcription factor 2, CRE-BP1, CREB-2, CREB2, CREBP1, Cyclic AMP-dependent transcription factor ATF-2, Cyclic AMP-responsive element-binding protein 2, HB16, cAMP response element-binding protein CRE-BP1, cAMP-dependent transcription factor ATF-2|
|Molecular Weight||approx. 55 to 75 kDa|
|Research Area||Immunology, Innate Immunity, Transcription Factors|
|Pathways||MAPK Signaling, RTK Signaling, TCR Signaling, Thyroid Hormone Synthesis, Activation of Innate immune Response, Chromatin Binding, Myometrial Relaxation and Contraction, Synaptic Membrane, Tube Formation, Toll-Like Receptors Cascades|
Application DetailsProduct Details anti-ATF2 Antibody Target Details ATF2 Handling Images back to top
ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200. IP: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-ATF2 Antibody Target Details ATF2 Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS), pH 7.2, 0.05 % Sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
ImagesProduct Details anti-ATF2 Antibody Target Details ATF2 Application Details Handling back to top