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anti-Dog (Canine) BAD antibody for Western Blotting

Recommended BAD Antibody (supplied by: Log in to see )

Antigen
BCL2-Associated Agonist of Cell Death (BAD) Antibodies
  • AI325008
  • bad
  • BAD
  • Bbc2
  • BBC2
  • BCL2L8
  • fa01b12
  • wu:fa01b12
  • wu:fa96d04
Epitope
C-Term
51
47
41
37
37
36
34
27
26
24
20
20
20
20
20
20
20
19
19
19
19
19
19
19
19
19
19
19
18
17
16
16
15
13
12
10
9
7
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
5
5
4
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
724
374
215
9
5
3
2
1
1
1
Host
Rabbit
918
49
2
1
Clonality
Polyclonal
Conjugate
This BAD antibody is un-conjugated
66
65
65
59
59
59
6
6
6
6
6
6
6
6
6
Application
Western Blotting (WB)
510
430
418
292
74
67
53
27
10
8
4
3
2
2
1
1
1
Supplier
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Catalog No. ABIN2779324
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.13357928 ABIN927291 WB Rabbit C-Term Log in to see Polyclonal

Top referenced anti-BAD antibody for Western Blotting

Similar anti-BAD Antibodies

Application / Reactivity Mouse (Murine) Human Rat (Rattus) Cow (Bovine) Dog (Canine)
Western Blotting (WB) 187 Antibodies 381 Antibodies 131 Antibodies 3 Antibodies 2 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Immunostaining (ISt) 1 Antibodies
Immunoprecipitation (IP) 36 Antibodies 37 Antibodies 15 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 65 Antibodies 36 Antibodies 25 Antibodies
Immunohistochemistry (IHC) 276 Antibodies 144 Antibodies 122 Antibodies 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies

General

Antigen BCL2-Associated Agonist of Cell Death (BAD) Antibodies
Epitope C-Term
(51), (47), (41), (37), (37), (36), (34), (27), (26), (24), (20), (20), (20), (20), (20), (20), (20), (19), (19), (19), (19), (19), (19), (19), (19), (19), (19), (19), (18), (17), (16), (16), (15), (13), (12), (10), (9), (7), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(724), (374), (215), (9), (5), (3), (2), (1), (1), (1)
Host Rabbit
(918), (49), (2), (1)
Clonality
Polyclonal
Conjugate This BAD antibody is un-conjugated
(66), (65), (65), (59), (59), (59), (6), (6), (6), (6), (6), (6), (6), (6), (6)
Application Western Blotting (WB)
(510), (430), (418), (292), (74), (67), (53), (27), (10), (8), (4), (3), (2), (2), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-BAD Antibody

Target Details BAD Application Details Handling References for anti-BAD Antibody (ABIN2779324) Images
Blocking Peptide Blocking peptide for this product available: ABIN973709
Sequence RMSDEFVDSF KKGLPRPKSA GTATQMRQSS SWTRVFQSWW DRNLGRGSSA
Predicted Reactivity Bovine : 100 %, Guinea pig : 100 %, Human : 100 %, Mouse : 100 %, Rabbit : 100 %, Rat : 100 %, Dog : 90 %, Horse : 90 %, Pig : 90 %
Characteristics This is a rabbit polyclonal antibody against BAD. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the C terminal region of human BAD

Target Details BAD

Product Details anti-BAD Antibody Application Details Handling References for anti-BAD Antibody (ABIN2779324) Images back to top
Antigen
Alternative Name BAD (BAD Antibody Abstract)
Background BAD is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform.The protein encoded by this gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform.
Molecular Weight 18 kDa
Gene ID 572
NCBI Accession NM_004322, NP_004313
UniProt Q92934
Research Area Apoptosis/Necrosis
Pathways MAPK Signaling, PI3K-Akt Signaling, RTK Signaling, Apoptosis, Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-BAD Antibody Target Details BAD Handling References for anti-BAD Antibody (ABIN2779324) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 168 AA

Restrictions For Research Use only

Handling

Product Details anti-BAD Antibody Target Details BAD Application Details References for anti-BAD Antibody (ABIN2779324) Images back to top
Format Liquid
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment For short term use, store at 2-8 °C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-BAD Antibody (ABIN2779324)

Product Details anti-BAD Antibody Target Details BAD Application Details Handling Images back to top
Background publications

Xia, Zhang, Du, Pan, Zhao, Sun, Hong, Liu, Fan: "miR-15b and miR-16 modulate multidrug resistance by targeting BCL2 in human gastric cancer cells." in: International journal of cancer. Journal international du cancer, Vol. 123, Issue 2, pp. 372-9, 2008

Images

Product Details anti-BAD Antibody Target Details BAD Application Details Handling References for anti-BAD Antibody (ABIN2779324) back to top
Supplier Images
Western Blotting (WB) image for anti-BAD antibody (BCL2-Associated Agonist of Cell Death) (C-Term) (ABIN2779324) anti-BCL2-Associated Agonist of Cell Death (BAD) (C-Term) antibody