anti-Dog (Canine) BAD antibody for Western Blotting

Recommended BAD Antibody (supplied by: Log in to see )

Antigen
BCL2-Associated Agonist of Cell Death (BAD) Antibodies
  • bad
  • fa01b12
  • wu:fa01b12
  • wu:fa96d04
  • BAD
  • BBC2
  • BCL2L8
  • AI325008
  • Bbc2
  • BCL2-antagonist of cell death b
  • BCL2-associated agonist of cell death
  • badb
  • BAD
  • Bad
Epitope
C-Term
60
58
53
50
48
46
43
29
22
22
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
19
19
19
19
15
15
12
10
9
8
8
7
5
5
5
5
4
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
789
436
264
18
15
7
4
3
2
2
1
1
1
1
1
1
1
Host
Rabbit
968
50
2
1
Clonality
Polyclonal
Conjugate
This BAD antibody is un-conjugated
65
64
64
58
58
58
6
6
6
6
6
6
6
6
6
6
6
Application
Western Blotting (WB)
529
473
396
296
125
79
55
30
13
12
6
5
4
3
2
1
1
1
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Catalog No. ABIN2779324
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.1467052 ABIN927291 WB Rabbit C-Term Log in to see Polyclonal
1 ABIN2738467 IHC (p) WB Rabbit Log in to see Polyclonal

General

Antigen BCL2-Associated Agonist of Cell Death (BAD) Antibodies
Epitope C-Term
(60), (58), (53), (50), (48), (46), (43), (29), (22), (22), (20), (20), (20), (20), (20), (20), (20), (20), (20), (20), (20), (20), (20), (20), (20), (19), (19), (19), (19), (15), (15), (12), (10), (9), (8), (8), (7), (5), (5), (5), (5), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
(789), (436), (264), (18), (15), (7), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(968), (50), (2), (1)
Clonality
Polyclonal
Conjugate This BAD antibody is un-conjugated
(65), (64), (64), (58), (58), (58), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6)
Application Western Blotting (WB)
(529), (473), (396), (296), (125), (79), (55), (30), (13), (12), (6), (5), (4), (3), (2), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-BAD Antibody

Target Details BAD Application Details Handling References for anti-BAD antibody (ABIN2779324) Images
Blocking Peptide Blocking peptide for this product available: ABIN973709
Sequence RMSDEFVDSF KKGLPRPKSA GTATQMRQSS SWTRVFQSWW DRNLGRGSSA
Predicted Reactivity Cow: 100%, Dog: 90%, Guinea Pig: 100%, Horse: 90%, Human: 100%, Mouse: 100%, Pig: 90%, Rabbit: 100%, Rat: 100%
Characteristics This is a rabbit polyclonal antibody against BAD. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the C terminal region of human BAD

Target Details BAD

Product Details anti-BAD Antibody Application Details Handling References for anti-BAD antibody (ABIN2779324) Images back to top
Antigen
Alternative Name BAD (BAD Antibody Abstract)
Background BAD is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform.The protein encoded by this gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform.
Alias Symbols: BBC2, BCL2L8
Protein Interaction Partner: BCL2L1, BCL2, AKT1, PAK1, BMH1, YWHAB, SFN, YWHAZ, RAF1, PRKACA, BRAF, ARAF, CREB3L3, STEAP3, YWHAQ, KEAP1, YWHAH, YWHAE, PRDX2, CDKN1A, VCP, SNCA, PPP1CA, BAX, SUMO2, YWHAG, PIM3, MCL1, RPS6KA5, PAK7, BCL2L10, WASF1, HRK, MAP2K5, BCL2L2, RPS6KA2, RPS6KA1
Protein Size: 168
Molecular Weight 18 kDa
Gene ID 572
NCBI Accession NM_004322, NP_004313
UniProt Q92934
Research Area Apoptosis/Necrosis
Pathways MAPK Signaling, PI3K-Akt Signaling, RTK Signaling, Apoptosis, Fc-epsilon Receptor Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Hepatitis C, CXCR4-mediated Signaling Events

Application Details

Product Details anti-BAD Antibody Target Details BAD Handling References for anti-BAD antibody (ABIN2779324) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 168 AA

Restrictions For Research Use only

Handling

Product Details anti-BAD Antibody Target Details BAD Application Details References for anti-BAD antibody (ABIN2779324) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-BAD antibody (ABIN2779324)

Product Details anti-BAD Antibody Target Details BAD Application Details Handling Images back to top
Background publications

Xia, Zhang, Du, Pan, Zhao, Sun, Hong, Liu, Fan: "miR-15b and miR-16 modulate multidrug resistance by targeting BCL2 in human gastric cancer cells." in: International journal of cancer. Journal international du cancer, Vol. 123, Issue 2, pp. 372-9, 2008

Images

Product Details anti-BAD Antibody Target Details BAD Application Details Handling References for anti-BAD antibody (ABIN2779324) back to top
Supplier Images
Western Blotting (WB) image for anti-BCL2-Associated Agonist of Cell Death (BAD) (C-Term) antibody (ABIN2779324) WB Suggested Anti-BAD Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:312500 Positive ...