anti-Human BAD antibody for Flow Cytometry

Recommended BAD Antibody (supplied by: Log in to see )

Antigen
BCL2-Associated Agonist of Cell Death (BAD) Antibodies
  • bad
  • fa01b12
  • wu:fa01b12
  • wu:fa96d04
  • BAD
  • BBC2
  • BCL2L8
  • AI325008
  • Bbc2
  • BCL2-antagonist of cell death b
  • BCL2-associated agonist of cell death
  • badb
  • BAD
  • Bad
Epitope
AA 92-127
60
58
53
50
48
46
43
29
22
22
21
20
20
20
20
20
20
20
20
20
20
20
20
20
20
19
19
19
19
15
15
12
10
9
8
8
7
5
5
5
4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
789
436
265
19
15
8
5
4
3
2
2
1
1
1
1
1
1
Host
Rabbit
968
50
2
1
Clonality
Polyclonal
Conjugate
This BAD antibody is un-conjugated
65
64
64
58
58
58
6
6
6
6
6
6
6
6
6
6
6
Application
Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
528
473
396
295
125
79
55
30
13
11
6
5
4
3
2
1
1
1
Supplier
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Catalog No. ABIN3030135
$ 411.13
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
14.356981 ABIN5542486 ELISA FACS WB Mouse IgG1 AA 1-168, full length Log in to see 1G5B3
1 ABIN388118 FACS IHC (p) WB Rabbit Ig AA 92-127, BH3 Domain Log in to see Polyclonal 3
1 ABIN957139 IHC (fro) FACS IHC (p) IP WB Rabbit Log in to see Polyclonal
1 ABIN4881163 ELISA FACS WB Mouse IgG1 Log in to see 1G5B3
1 ABIN4902999 FACS ICC IHC IP WB Rabbit IgG Log in to see
1 ABIN1841063 FACS IHC (p) WB Rabbit AA 92-127 Log in to see Polyclonal
1 ABIN4282855 FACS WB Rabbit Log in to see Polyclonal
1 ABIN4282854 FACS WB Rabbit Log in to see Polyclonal
1 ABIN5624354 FACS Rabbit IgG1 kappa pSer112 Log in to see BADS112-B9
1 ABIN3059653 FACS IF/ICC IHC IP WB Rabbit Log in to see Polyclonal

General

Antigen BCL2-Associated Agonist of Cell Death (BAD) Antibodies
Epitope AA 92-127
(60), (58), (53), (50), (48), (46), (43), (29), (22), (22), (21), (20), (20), (20), (20), (20), (20), (20), (20), (20), (20), (20), (20), (20), (20), (19), (19), (19), (19), (15), (15), (12), (10), (9), (8), (8), (7), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(789), (436), (265), (19), (15), (8), (5), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Host Rabbit
(968), (50), (2), (1)
Clonality
Polyclonal
Conjugate This BAD antibody is un-conjugated
(65), (64), (64), (58), (58), (58), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6)
Application Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(528), (473), (396), (295), (125), (79), (55), (30), (13), (11), (6), (5), (4), (3), (2), (1), (1), (1)
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Product Details anti-BAD Antibody

Target Details BAD Application Details Handling Images
Purification Purified
Immunogen A portion of amino acids 92-127 from the human protein was used as the immunogen for this Bad antibody.
Isotype Ig Fraction

Target Details BAD

Product Details anti-BAD Antibody Application Details Handling Images back to top
Antigen
Alternative Name Bad (BAD Antibody Abstract)
Background Apoptosis or programmed cell death is a physiological cellular process characterized by cell shrinkage, membrane blebbing, DNA fragmentation, and release of Cytochrome C from the mitochondria. It is utilized by the organism to get rid of unwanted cells, which is critical for normal development and homeostasis of an organism. Disregulation of normal apoptosis process have been implicated in a variety of diseases, including cancer, autoimmune diseases, viral infections, etc. Programmed cell death occurs through complex cascades of cell signaling in which Bcl-2 family members, among others, play an important role.The Bcl-2 family of proteins regulate apoptosis as well as execute death signals at the mitochondrion. Members of this family include both pro- and anti-apoptotic proteins that hare homology sequences called Bcl-2 Homology domains (BH1-4) which mediate dimmer formation. The BH3 proteins, such as BID, NOXA, PUMA, BIK, BIM and BAD are all pro-apoptotic and share sequence homology within the amphipathic alpha-helical BH3 region, which is required for their apoptotic function. They may trigger release of death-inducing molecules such as Cytochrome C, Smac, and endonuclease G. Anti-apoptotic family members, including Bcl-2 and Bcl-XL, play inhibitory roles. Bcl-2 family proteins may form homodimers or heterodimers between pro- and anti-apoptotic members, the ratios of which determine the cell fate.
UniProt Q92934
Research Area Cancer, Apoptosis/Necrosis
Pathways MAPK Signaling, PI3K-Akt Signaling, RTK Signaling, Apoptosis, Fc-epsilon Receptor Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Hepatitis C, CXCR4-mediated Signaling Events

Application Details

Product Details anti-BAD Antibody Target Details BAD Handling Images back to top
Application Notes Titration of the Bad antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Flow Cytometry: 1:10-1:50
Restrictions For Research Use only

Handling

Product Details anti-BAD Antibody Target Details BAD Application Details Images back to top
Format Liquid
Buffer In 1X PBS, pH 7.4, with 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Aliquot the Bad antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

Product Details anti-BAD Antibody Target Details BAD Application Details Handling back to top
Supplier Images
Flow Cytometry (FACS) image for anti-BCL2-Associated Agonist of Cell Death (BAD) (AA 92-127) antibody (ABIN3030135) Bad antibody flow cytometric analysis of HeLa cells (right histogram) compared to a n...
Immunohistochemistry (IHC) image for anti-BCL2-Associated Agonist of Cell Death (BAD) (AA 92-127) antibody (ABIN3030135) IHC analysis of FFPE human hepatocarcinoma tissue stained with the Bad antibody
Western Blotting (WB) image for anti-BCL2-Associated Agonist of Cell Death (BAD) (AA 92-127) antibody (ABIN3030135) Western blot analysis of Bad antibody and mouse bladder tissue lysate