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anti-Rat (Rattus) BAD antibody for Immunocytochemistry

Recommended BAD Antibody (supplied by: Log in to see )

Antigen
BCL2-Associated Agonist of Cell Death (BAD) Antibodies
  • AI325008
  • bad
  • BAD
  • Bbc2
  • BBC2
  • BCL2L8
  • fa01b12
  • wu:fa01b12
  • wu:fa96d04
Epitope
N-Term
51
45
41
37
36
36
34
27
27
23
20
20
20
20
20
20
20
19
19
19
19
19
19
19
19
19
19
19
18
17
16
16
15
13
12
10
8
7
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
5
5
4
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
711
365
208
9
5
4
2
2
1
Host
Rabbit
904
48
2
1
Clonality
Monoclonal
Conjugate
This BAD antibody is un-conjugated
66
65
65
59
59
59
6
6
6
6
6
6
6
6
6
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
509
418
416
279
67
60
46
23
8
4
4
3
2
2
1
1
1
Supplier
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Catalog No. ABIN268326
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010161072 ABIN446783 ICC IF IHC (p) IHC WB Rabbit AA 110-160 Log in to see Polyclonal
0.0010161072 ABIN2736342 ICC FACS IP IHC WB Rabbit IgG Log in to see Polyclonal
0.0010161072 ABIN3059653 ICC FACS IF IP IHC WB Rabbit Log in to see Polyclonal

Similar anti-BAD Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
Dot Blot (DB) 36 Antibodies 132 Antibodies
ELISA 80 Antibodies 163 Antibodies
Flow Cytometry (FACS) 3 Antibodies 7 Antibodies
Immunocytochemistry (ICC) 4 Antibodies 4 Antibodies
Immunofluorescence (IF) 14 Antibodies 14 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 33 Antibodies 34 Antibodies
Immunohistochemistry (IHC) 114 Antibodies 135 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 17 Antibodies
Immunoprecipitation (IP) 11 Antibodies
Western Blotting (WB) 123 Antibodies

General

Antigen BCL2-Associated Agonist of Cell Death (BAD) Antibodies
Epitope N-Term
(51), (45), (41), (37), (36), (36), (34), (27), (27), (23), (20), (20), (20), (20), (20), (20), (20), (19), (19), (19), (19), (19), (19), (19), (19), (19), (19), (19), (18), (17), (16), (16), (15), (13), (12), (10), (8), (7), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(711), (365), (208), (9), (5), (4), (2), (2), (1)
Host Rabbit
(904), (48), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This BAD antibody is un-conjugated
(66), (65), (65), (59), (59), (59), (6), (6), (6), (6), (6), (6), (6), (6), (6)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(509), (418), (416), (279), (67), (60), (46), (23), (8), (4), (4), (3), (2), (2), (1), (1), (1)
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Product Details anti-BAD Antibody

Target Details BAD Application Details Handling Images
Specificity The antibody does not cross-react with other Bcl-2 members.
Purification IgG purified
Immunogen A synthetic peptide corresponding to residues in the N-term of human Bad was used as immunogen.
Clone Y208
Isotype IgG

Target Details BAD

Product Details anti-BAD Antibody Application Details Handling Images back to top
Antigen
Alternative Name Bcl-2-like 8 (BAD Antibody Abstract)
Background Bad is a pro-apoptotic member of the Bcl-2 family. Bad will bind preferentially Bcl-xLover Bcl-2 through its partial (9-16 a.a) BH3 domain. Additionally, Bad can reverse thedeath repressor activity of Bcl-xL but not of Bcl-2. Upon dephosphorylation, activatedBad translocates to the mitochondria and displace Bcl-2 from tBid sequestration toinitiate mitochondrial dysfunction. The switching on/off of its phosphorylation bygrowth/survival factors regulates Bad activity. Alternate Names: anti-Bcl-2 binding component 6 antibody, anti-BBC 2 antibody, anti-BBC2 antibody,anti-BBC6 antibody, anti-Bcl 2 Antagonist of Cell Death antibody, anti-Bcl 2 BindingComponent 6 antibody, anti-BCL X / BCL 2 Binding Protein antibody, anti-BCL XBinding Protein antibody, anti-Bcl XL/Bcl 2 Associated Death Promoter antibody,anti-Bcl2 Associated Death Promoter antibody, anti-Bcl2 Like 8 Protein antibody,anti-BCL2L8 antibody, anti-BclXL antibody, anti-Proapoptotic BH3 Only Protein antibody.
Gene Symbol: BAD
Gene ID 572, 12015, 64639
Research Area Apoptosis/Necrosis
Pathways MAPK Signaling, PI3K-Akt Signaling, RTK Signaling, Apoptosis, Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-BAD Antibody Target Details BAD Handling Images back to top
Application Notes This product is useful for: Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunoprecipitation, Flow Cytometry. In Western blot this antibody detects a band at approximately 23kDa.
Recommended dilutions: Flow Cytometry 1:50, Immunocytochemistry/Immunofluorescence 1:250-500, Immunohistochemistry 1:10-1:2000, Immunohistochemistry-Paraffin 1:100-250, Immunoprecipitation 1:100, Western Blot 1:1000-10000
Protocol Protocol specific for Bad Antibody Immunohistochemistry Protocol for Paraffin-embedded Tissues
1. Solutions and reagents1.
. Xylene1.
. Ethanol, anhydrous denatured, histological grade (100 %, 95 %, 70 %)1.
. Washing buffer:TBST washing buffer: 1XTBS/0.1 % Tween-20To prepare stock solution of 10X TBS: add 24.2 g Trizma base and 80 g sodium chloride to 1L of dH2O. Adjust pH to 7.6.Working solution. 1XTBST/0.1 % Tween-20: add 100ml 10XTBS to 900 mL dH2O. Add 1 mL Tween-20 and mix well.1.
. Distilled water (dH2O)1.
. Antigen Retrieval Solution:0.01M Sodium Citrate
Restrictions For Research Use only

Handling

Product Details anti-BAD Antibody Target Details BAD Application Details Images back to top
Format Liquid
Buffer PBS 49 % , Glycerol 50 % , BSA 0.05 % , Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment -20 °C.