|Antigen||B-Cell CLL/lymphoma 2 (BCL2) Antibodies|
|Reactivity||Dog (Canine), Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This BCL2 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Western Blotting (WB)
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Product Details anti-BCL2 AntibodyTarget Details BCL2 Application Details Handling Images
|Specificity||This antibody is expected to recognise both reported isoforms (NP_000624, NP_000648) of BCL2.|
Species reactivity (expected):Mouse, Rat, Canine.
Species reactivity (tested):Human.
|Purification||Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.|
|Immunogen||Peptide with sequence from the N Terminus of the protein sequence according to NP_000624.2, NP_000648.2. Genename: BCL2|
Target Details BCL2Product Details anti-BCL2 Antibody Application Details Handling Images back to top
|Alternative Name||Bcl-2 (BCL2 Antibody Abstract)|
|Background||BCL2 is an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants (alpha and beta) produced by alternate splicing, differ in their C-terminal ends. BCL2 suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. It regulates cell death by controlling the mitochondrial membrane permeability. It appears to function in a feedback loop system with caspases. BCL2 inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF1). It can form homodimers, and heterodimers with BAX, BAD, BAK and BclX(L). Heterodimerization with BAX requires intact BH1 and BH2 domains, and is necessary for anti-apoptotic activity. Also interacts with APAF1, RAF1, TP53BP2, BBC3, BCL2L1 and BNIPL.Synonyms: Apoptosis regulator Bcl-2, B-Cell CLL/lymphoma 2, BCL2, Bcl-2 alpha|
|Gene ID||596, 9606|
|Research Area||Apoptosis/Necrosis, Cancer|
|Pathways||MAPK Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling|
Application DetailsProduct Details anti-BCL2 Antibody Target Details BCL2 Handling Images back to top
Peptide ELISA: 1/32000. Western blot: 1 - 3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-BCL2 Antibody Target Details BCL2 Application Details Images back to top
|Buffer||Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|