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anti-Human BCL2 antibody for ELISA

Recommended BCL2 Antibody (supplied by: Log in to see )

Antigen
B-Cell CLL/lymphoma 2 (BCL2) Antibodies
  • AW986256
  • Bcl-2
  • C430015F12Rik
  • D630044D05Rik
  • D830018M01Rik
  • PPP1R50
  • BCL-2
  • bcl-2
  • Bcl2
  • B-cell CLL/lymphoma 2
  • B cell leukemia/lymphoma 2
  • B-cell CLL/lymphoma 2 protein
  • B-cell leukemia/lymphoma 2
  • BCL2
  • LOC100224888
  • Bcl2
  • BCL-2
  • bcl2
Epitope
N-Term
165
73
63
57
41
33
25
25
24
18
15
15
15
14
13
13
13
11
8
7
6
5
5
5
5
5
4
3
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
982
249
209
54
28
22
18
18
5
4
4
3
2
2
2
2
Host
Goat
533
511
18
6
2
2
2
Clonality
Polyclonal
Conjugate
This BCL2 antibody is un-conjugated
47
46
42
37
24
23
21
15
14
13
13
13
13
13
13
13
12
12
12
7
7
7
6
6
6
6
6
6
6
2
2
1
1
1
1
1
1
1
1
1
1
1
Method Type
Peptide ELISA
Application
ELISA, Western Blotting (WB)
779
367
359
307
305
289
226
185
169
81
40
13
12
12
11
9
9
4
3
1
1
1
1
1
1
1
Options
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Catalog No. ABIN185269
$ 326.89
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1531302 IHC ELISA WB Rabbit IgG pSer87 Log in to see Polyclonal 1
1 ABIN1531787 IF IHC ELISA WB Rabbit IgG pThr56 Log in to see Polyclonal 1
1 ABIN1531786 IHC ELISA WB Rabbit IgG pSer70 Log in to see Polyclonal 1
1 ABIN1532816 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 1
1 ABIN268671 ELISA WB Goat N-Term Log in to see Polyclonal 4
1 ABIN968975 ICC FACS IHC ELISA Mouse IgG1 Log in to see 8-00E-12 3
1 ABIN1582440 ICC IHC ELISA WB Mouse IgG1 N-Term Log in to see 1
1 ABIN4283376 ELISA FACS ICC IF IHC IHC (p) Mouse IgG1 Log in to see 8E12 1
1 ABIN3182532 ELISA IHC WB Rabbit IgG pSer87 Log in to see Polyclonal
1 ABIN3181931 ELISA IF IHC IP WB Rabbit IgG pThr56 Log in to see Polyclonal
1 ABIN3181930 ELISA IHC IP WB Rabbit IgG pSer70 Log in to see Polyclonal
1 ABIN3183499 ELISA IF IHC WB Rabbit IgG Ser172 Log in to see Polyclonal
1 ABIN3183497 ELISA IHC WB Rabbit IgG Ser167 Log in to see Polyclonal
1 ABIN3183498 ELISA IF IHC WB Rabbit IgG Ser168, Ser170 Log in to see Polyclonal
1 ABIN1531491 IF IHC ELISA Rabbit IgG pThr69 Log in to see Polyclonal
1 ABIN2862600 IF IP IHC ELISA WB Rabbit IgG pThr56 Log in to see Polyclonal
1 ABIN2862602 IP IHC ELISA WB Rabbit IgG pSer70 Log in to see Polyclonal
1 ABIN4283406 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB HRP Mouse IgG1 kappa Log in to see 8C8
1 ABIN1532817 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN1532505 IF IHC ELISA WB Rabbit IgG Log in to see Polyclonal

Top referenced anti-BCL2 antibody for ELISA

Similar anti-BCL2 Antibodies

Application / Reactivity Human
Cell Culture (CC) 1 Antibodies
Cellular Assay (CA) 1 Antibodies
Cytometry by Time of Flight (CyTOF) 12 Antibodies
Dot Blot (DB) 40 Antibodies
ELISA 343 Antibodies
ELISA (Detection) 1 Antibodies
Enzyme Immunoassay (EIA) 8 Antibodies
Flow Cytometry (FACS) 289 Antibodies
Immunoassay (IA) 1 Antibodies
Immunochromatography (IC) 9 Antibodies
Immunocytochemistry (ICC) 171 Antibodies
Immunofluorescence (IF) 291 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 12 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 68 Antibodies
Immunohistochemistry (IHC) 278 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 11 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 166 Antibodies

General

Antigen B-Cell CLL/lymphoma 2 (BCL2) Antibodies
Epitope N-Term
(165), (73), (63), (57), (41), (33), (25), (25), (24), (18), (15), (15), (15), (14), (13), (13), (13), (11), (8), (7), (6), (5), (5), (5), (5), (5), (4), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(982), (249), (209), (54), (28), (22), (18), (18), (5), (4), (4), (3), (2), (2), (2), (2)
Host Goat
(533), (511), (18), (6), (2), (2), (2)
Clonality
Polyclonal
Conjugate This BCL2 antibody is un-conjugated
(47), (46), (42), (37), (24), (23), (21), (15), (14), (13), (13), (13), (13), (13), (13), (13), (12), (12), (12), (7), (7), (7), (6), (6), (6), (6), (6), (6), (6), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Method Type Peptide ELISA
Application ELISA, Western Blotting (WB)
(779), (367), (359), (307), (305), (289), (226), (185), (169), (81), (40), (13), (12), (12), (11), (9), (9), (4), (3), (1), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-BCL2 Antibody

Target Details BCL2 Application Details Handling References for anti-BCL2 Antibody (ABIN185269) Images
Blocking Peptide Blocking peptide for this product available: ABIN368747
Specificity This antibody is expected to recognise both reported isoforms (NP_000624, NP_000648)
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen Peptide with sequence AGRTGYDNREIVMKYC, from the N Terminus of the protein sequence according to NP_000624.2, NP_000648.2.

Target Details BCL2

Product Details anti-BCL2 Antibody Application Details Handling References for anti-BCL2 Antibody (ABIN185269) Images back to top
Antigen
Alternative Name BCL2 (BCL2 Antibody Abstract)
Background Synonyms: BCL2, Bcl-2, B-cell CLL/lymphoma 2, B-cell lymphoma protein 2, OTTHUMP00000163680
Gene ID 596, 12043, 24224
NCBI Accession NP_000648, NP_000624
Pathways MAPK Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling

Application Details

Product Details anti-BCL2 Antibody Target Details BCL2 Handling References for anti-BCL2 Antibody (ABIN185269) Images back to top
Application Notes ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 27+25 kDa band observed in Human Lymph lysates (calculated MW of 26.3 kDa according to NP_000624.2 and of 22.2 kDa according to NP_000648.2). Recommended concentration: 0.1-0.3 µg/mL.
Restrictions For Research Use only

Handling

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details References for anti-BCL2 Antibody (ABIN185269) Images back to top
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.

References for anti-BCL2 Antibody (ABIN185269)

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details Handling Images back to top
Background publications

Reed: "Double identity for proteins of the Bcl-2 family." in: Nature, Vol. 387, Issue 6635, pp. 773-6, 1997

Images

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details Handling References for anti-BCL2 Antibody (ABIN185269) back to top
Supplier Images
Western Blotting (WB) image for anti-BCL2 antibody (B-Cell CLL/lymphoma 2) (N-Term) (ABIN185269) ABIN185269 staining (1µg/ml) of Human Lymph Node lysate (RIPA buffer, 35µg total prot...