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anti-Human BCL2 antibody for Immunofluorescence

Recommended BCL2 Antibody (supplied by: Log in to see )

Antigen
B-Cell CLL/lymphoma 2 (BCL2) Antibodies
  • AW986256
  • bcl-2
  • BCL-2
  • Bcl-2
  • Bcl2
  • C430015F12Rik
  • D630044D05Rik
  • D830018M01Rik
  • PPP1R50
Epitope
AA 75-110, BH3 Domain
76
71
53
50
48
44
23
23
19
17
15
14
14
13
13
13
13
12
11
9
7
4
4
4
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
673
200
151
17
9
7
4
3
2
2
1
1
Host
Rabbit
493
240
19
5
2
2
2
Clonality
Polyclonal
Conjugate
This BCL2 antibody is un-conjugated
38
33
28
24
19
14
11
10
7
6
6
6
6
6
6
3
1
1
1
1
Application
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
480
275
241
144
138
138
112
67
49
42
33
11
10
10
8
8
5
1
1
1
1
1
Supplier
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Catalog No. ABIN388095
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.3161046 ABIN1531787 IF IHC ELISA WB Rabbit IgG pThr56 Log in to see Polyclonal 1
0.3161046 ABIN499429 EIA IF WB Rabbit IgG Intermediate Domain Log in to see Polyclonal 1
0.3161046 ABIN950625 EIA IF IHC (p) WB Rabbit IgG AA 145-157, Internal Region Log in to see Polyclonal
0.3161046 ABIN1531491 IF IHC ELISA Rabbit IgG pThr69 Log in to see Polyclonal
0.3161046 ABIN1538273 IF WB Rabbit Ig AA 300-327, Center Log in to see Polyclonal
0.3161046 ABIN1532505 IF IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.3161046 ABIN2705579 IC IF IHC WB Rabbit Center Log in to see Polyclonal
0.3161046 ABIN2705577 IC IF IHC WB Rabbit Center Log in to see Polyclonal
0.3161046 ABIN2167716 FACS IF IHC ELISA Mouse IgG1 Log in to see 8-00E-012
0.3161046 ABIN934065 IF ELISA WB Mouse IgG2b kappa AA 1-211 Log in to see AT1B5
0.3161046 ABIN567617 EIA IF WB Mouse IgG2b Log in to see AT1B5
0.3161046 ABIN2162973 IF IHC ELISA WB Rabbit IgG Thr69 Log in to see Polyclonal
0.3161046 ABIN2156057 ELISA IF IHC WB Rabbit IgG N-Term Log in to see Polyclonal
0.3161046 ABIN2159485 IF IHC Rabbit Ig Fraction Log in to see Polyclonal
0.3161046 ABIN1105505 EIA FACS IF IHC (p) WB Mouse IgG1 Log in to see 8-00E-012
0.3161046 ABIN2405616 IF WB Rabbit IgG Log in to see Polyclonal
0.3161046 ABIN2403428 IF IHC WB Rabbit IgG Log in to see Polyclonal
0.0010850558 ABIN1105504 IHC (fro) IF IHC (p) WB Mouse IgG1 AA 41-54 Log in to see BL-2 2
0.0010850558 ABIN968024 IF IP IHC WB Mouse IgG1 AA 49-179 Log in to see 7-Bcl 5
0.0010850558 ABIN968025 IF IP IHC WB Mouse IgG1 AA 49-179 Log in to see 7-Bcl 5

Top referenced anti-BCL2 antibody for Immunofluorescence

Similar anti-BCL2 Antibodies

Application / Reactivity Human
Cellular Assay (CA) 1 Antibodies
Dot Blot (DB) 42 Antibodies
ELISA 246 Antibodies
Enzyme Immunoassay (EIA) 9 Antibodies
Flow Cytometry (FACS) 116 Antibodies
Immunoassay (IA) 1 Antibodies
Immunochromatography (IC) 10 Antibodies
Immunocytochemistry (ICC) 51 Antibodies
Immunofluorescence (IF) 146 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 56 Antibodies
Immunohistochemistry (IHC) 240 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 8 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 31 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 112 Antibodies
Immunoprecipitation (IP) 132 Antibodies
Immunostaining (ISt) 1 Antibodies
Intracellular Staining (ICS) 3 Antibodies

General

Antigen B-Cell CLL/lymphoma 2 (BCL2) Antibodies
Epitope AA 75-110, BH3 Domain
(76), (71), (53), (50), (48), (44), (23), (23), (19), (17), (15), (14), (14), (13), (13), (13), (13), (12), (11), (9), (7), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(673), (200), (151), (17), (9), (7), (4), (3), (2), (2), (1), (1)
Host Rabbit
(493), (240), (19), (5), (2), (2), (2)
Clonality (Clone)
Polyclonal   ( )
Conjugate This BCL2 antibody is un-conjugated
(38), (33), (28), (24), (19), (14), (11), (10), (7), (6), (6), (6), (6), (6), (6), (3), (1), (1), (1), (1)
Application Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(480), (275), (241), (144), (138), (138), (112), (67), (49), (42), (33), (11), (10), (10), (8), (8), (5), (1), (1), (1), (1), (1)
Pubmed 19 references available
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Product Details anti-BCL2 Antibody

Target Details BCL2 Application Details Handling References for anti-BCL2 Antibody (ABIN388095) Images
Specificity This Bcl-2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 85~115 amino acids from the BH3 domain of human Bcl2.
Predicted Reactivity Cow (Bovine)
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Immunogen This Bcl antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 75-110 AA from human Bcl.
Clone RB04684
Isotype Ig

Target Details BCL2

Product Details anti-BCL2 Antibody Application Details Handling References for anti-BCL2 Antibody (ABIN388095) Images back to top
Antigen
Alternative Name Bcl-2 (BCL2 Antibody Abstract)
Background BCL2 is integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma.
Synonyms: Apoptosis regulator Bcl-2, B-cell lymphoma protein 2
Pathways: Neurotrophin signaling pathway, Toxoplasmosis, Pathways in cancer, Protein processing in endoplasmic reticulum, Tuberculosis, Focal adhesion, Prostate cancer, Small cell lung cancer, Apoptosis, Colorectal cancer, Amyotrophic lateral sclerosis (ALS)
Molecular Weight 26266 Da
Gene ID 596
UniProt P10415
Research Area Apoptosis/Necrosis, Cell Structure, Cancer
Pathways MAPK Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-BCL2 Antibody Target Details BCL2 Handling References for anti-BCL2 Antibody (ABIN388095) Images back to top
Application Notes IHC (p) = 1:10-50, IF = 1:10-50, WB = 1:1000
Restrictions For Research Use only

Handling

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details References for anti-BCL2 Antibody (ABIN388095) Images back to top
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-BCL2 Antibody (ABIN388095)

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details Handling Images back to top
Product cited in:

Kiss, Tóth, Sarang, Garabuczi, Szondy: "Retinoids induce Nur77-dependent apoptosis in mouse thymocytes." in: Biochimica et biophysica acta, Vol. 1853, Issue 3, pp. 660-70, 2015

Hu, Baldwin: "Differential roles for Bim and Nur77 in thymocyte clonal deletion induced by ubiquitous self-antigen." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 194, Issue 6, pp. 2643-53, 2015

Cai, Xia, Yang, Liu, Shao, Liu, Yang, Xiong: "Ropivacaine- and bupivacaine-induced death of rabbit annulus fibrosus cells in vitro: involvement of the mitochondrial apoptotic pathway." in: Osteoarthritis and cartilage / OARS, Osteoarthritis Research Society, 2015

Li, Wang, Wang, Gao, Zhang, Lu: "Oridonin phosphate-induced autophagy effectively enhances cell apoptosis of human breast cancer cells." in: Medical oncology (Northwood, London, England), Vol. 32, Issue 1, pp. 365, 2014

Pickles, Destroismaisons, Peyrard, Cadot, Rouleau, Brown, Julien, Arbour, Vande Velde: "Mitochondrial damage revealed by immunoselection for ALS-linked misfolded SOD1." in: Human molecular genetics, Vol. 22, Issue 19, pp. 3947-59, 2013

Chiavarina, Whitaker-Menezes, Martinez-Outschoorn, Witkiewicz, Birbe, Howell, Pestell, Smith, Daniel, Sotgia, Lisanti: "Pyruvate kinase expression (PKM1 and PKM2) in cancer-associated fibroblasts drives stromal nutrient production and tumor growth." in: Cancer biology & therapy, Vol. 12, Issue 12, pp. 1101-13, 2012

Zhao, He, Liu, Zhu, Tian, Gao, He, Yue, Lei, Ni, Wang, Jin, Hao, Lin, Chen: "Natural diterpenoid compound elevates expression of Bim protein, which interacts with antiapoptotic protein Bcl-2, converting it to proapoptotic Bax-like molecule." in: The Journal of biological chemistry, Vol. 287, Issue 2, pp. 1054-65, 2012

Guareschi, Cova, Cereda, Ceroni, Donetti, Bosco, Trotti, Pasinelli: "An over-oxidized form of superoxide dismutase found in sporadic amyotrophic lateral sclerosis with bulbar onset shares a toxic mechanism with mutant SOD1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 13, pp. 5074-9, 2012

Pedrini, Sau, Guareschi, Bogush, Brown, Naniche, Kia, Trotti, Pasinelli: "ALS-linked mutant SOD1 damages mitochondria by promoting conformational changes in Bcl-2." in: Human molecular genetics, Vol. 19, Issue 15, pp. 2974-86, 2010

Ferlini, Cicchillitti, Raspaglio, Bartollino, Cimitan, Bertucci, Mozzetti, Gallo, Persico, Fattorusso, Campiani, Scambia: "Paclitaxel directly binds to Bcl-2 and functionally mimics activity of Nur77." in: Cancer research, Vol. 69, Issue 17, pp. 6906-14, 2009

Deng, Gao, May: "Protein phosphatase 2A inactivates Bcl2's antiapoptotic function by dephosphorylation and up-regulation of Bcl2-p53 binding." in: Blood, Vol. 113, Issue 2, pp. 422-8, 2009

Kolluri, Zhu, Zhou, Lin, Chen, Sun, Tian, Town, Cao, Lin, Zhai, Kitada, Luciano, ODonnell, Cao, He, Lin, Reed, Satterthwait, Zhang: "A short Nur77-derived peptide converts Bcl-2 from a protector to a killer." in: Cancer cell, Vol. 14, Issue 4, pp. 285-98, 2008

Thompson, Winoto: "During negative selection, Nur77 family proteins translocate to mitochondria where they associate with Bcl-2 and expose its proapoptotic BH3 domain." in: The Journal of experimental medicine, Vol. 205, Issue 5, pp. 1029-36, 2008

Del Gaizo Moore, Schlis, Sallan, Armstrong, Letai: "BCL-2 dependence and ABT-737 sensitivity in acute lymphoblastic leukemia." in: Blood, Vol. 111, Issue 4, pp. 2300-9, 2008

Liu, Zhou, Li, Sun, Lin, Lang, He, Cao, Yan, Wang, Lu, Han, Cao, Zhang, Zeng: "Modulation of orphan nuclear receptor Nur77-mediated apoptotic pathway by acetylshikonin and analogues." in: Cancer research, Vol. 68, Issue 21, pp. 8871-80, 2008

Lei, Chen, Du, Yu, Wang, Qu, Xia, He, Mao, Zong, Liao, Mehrpour, Hao, Chen: "Gossypol induces Bax/Bak-independent activation of apoptosis and cytochrome c release via a conformational change in Bcl-2." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 20, Issue 12, pp. 2147-9, 2006

Background publications

Zheng, Zhou, Ye, Li, Bai, Zhang: "The conformation change of Bcl-2 is involved in arsenic trioxide-induced apoptosis and inhibition of proliferation in SGC7901 human gastric cancer cells." in: World journal of surgical oncology, Vol. 8, Issue 1, pp. 31, 2010

Thoulouze, Lafage, Yuste, Baloul, Edelman, Kroemer, Israel, Susin, Lafon: "High level of Bcl-2 counteracts apoptosis mediated by a live rabies virus vaccine strain and induces long-term infection." in: Virology, Vol. 314, Issue 2, pp. 549-61, 2003

Iwata, Stevenson, Minard, Tasch, Tupper, Lagasse, Weissman, Harlan, Winn: "Over-expression of Bcl-2 provides protection in septic mice by a trans effect." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 171, Issue 6, pp. 3136-41, 2003

Images

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details Handling References for anti-BCL2 Antibody (ABIN388095) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-BCL2 antibody (B-Cell CLL/lymphoma 2) (AA 75-110) (ABIN388095) Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with Bcl-2 ...
Western Blotting (WB) image for anti-BCL2 antibody (B-Cell CLL/lymphoma 2) (AA 75-110) (ABIN388095) Left panel, analysis of Bcl-2 BH3 domain exposure in HEK293 cells transfected with a ...
Immunofluorescence (IF) image for anti-BCL2 antibody (B-Cell CLL/lymphoma 2) (AA 75-110) (ABIN388095) Confocal immunofluorescent analysis of Bcl-2 Antibody (BH3 Domain Specific) (ABIN3880...