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anti-Human BCL2 antibody for Immunohistochemistry

Recommended BCL2 Antibody (supplied by: Log in to see )

Antigen
B-Cell CLL/lymphoma 2 (BCL2) Antibodies
  • AW986256
  • bcl-2
  • BCL-2
  • Bcl-2
  • Bcl2
  • C430015F12Rik
  • D630044D05Rik
  • D830018M01Rik
  • PPP1R50
Epitope
pThr56
169
71
61
53
50
44
23
23
23
19
17
15
14
14
13
13
13
13
12
12
7
4
4
4
3
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
954
221
166
40
23
16
7
4
3
2
2
2
1
1
Host
Rabbit
524
504
19
6
2
2
2
Clonality
Polyclonal
Conjugate
This BCL2 antibody is un-conjugated
47
45
40
36
24
23
19
14
13
13
13
13
13
13
13
13
12
12
12
12
7
6
6
6
6
6
6
3
1
1
1
1
Application
Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
764
341
322
302
291
281
257
181
153
67
42
11
10
10
8
8
5
1
1
1
1
1
Supplier
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Catalog No. ABIN1531787
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.21730916 ABIN1531786 IHC ELISA WB Rabbit IgG pSer70 Log in to see Polyclonal 1
0.21730916 ABIN1531302 IHC ELISA WB Rabbit IgG pSer87 Log in to see Polyclonal 1
0.21730916 ABIN2792106 IHC WB Rabbit N-Term Log in to see Polyclonal 1
0.21730916 ABIN1532816 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 1
0.21730916 ABIN968975 ICC FACS IHC ELISA Mouse IgG1 Log in to see 8-00E-12 3
0.21730916 ABIN1531491 IF IHC ELISA Rabbit IgG pThr69 Log in to see Polyclonal
0.21730916 ABIN1532505 IF IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.21730916 ABIN1532817 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.21730916 ABIN1870013 IHC Rabbit IgG pThr56 Log in to see Polyclonal
0.21730916 ABIN2469588 IHC WB Rabbit IgG Thr56 Log in to see Polyclonal
0.21730916 ABIN2469589 IHC Rabbit IgG Ser70 Log in to see Polyclonal
0.21730916 ABIN2705575 IHC WB Rabbit Center Log in to see Polyclonal
0.21730916 ABIN2705576 IP IHC WB Rabbit pSer70 Log in to see Polyclonal
0.21730916 ABIN2705579 IC IF IHC WB Rabbit Center Log in to see Polyclonal
0.21730916 ABIN2705574 IHC WB Rabbit pSer69 Log in to see Polyclonal
0.21730916 ABIN2705577 IC IF IHC WB Rabbit Center Log in to see Polyclonal
0.21730916 ABIN2705578 IHC WB Rabbit pSer87 Log in to see Polyclonal
0.21730916 ABIN1870012 IHC Rabbit IgG pSer70 Log in to see Polyclonal
0.21730916 ABIN2162960 IF IHC WB Rabbit IgG Ser56, Thr56 Log in to see Polyclonal
0.21730916 ABIN2162958 IHC Rabbit IgG Thr56 Log in to see Polyclonal

Top referenced anti-BCL2 antibody for Immunohistochemistry

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Application / Reactivity Human
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Flow Cytometry (FACS) 279 Antibodies
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Immunofluorescence (IF) 275 Antibodies
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Immunohistochemistry (IHC) 274 Antibodies
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Immunostaining (ISt) 1 Antibodies
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General

Antigen B-Cell CLL/lymphoma 2 (BCL2) Antibodies
Epitope pThr56
(169), (71), (61), (53), (50), (44), (23), (23), (23), (19), (17), (15), (14), (14), (13), (13), (13), (13), (12), (12), (7), (4), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(954), (221), (166), (40), (23), (16), (7), (4), (3), (2), (2), (2), (1), (1)
Host Rabbit
(524), (504), (19), (6), (2), (2), (2)
Clonality
Polyclonal
Conjugate This BCL2 antibody is un-conjugated
(47), (45), (40), (36), (24), (23), (19), (14), (13), (13), (13), (13), (13), (13), (13), (13), (12), (12), (12), (12), (7), (6), (6), (6), (6), (6), (6), (3), (1), (1), (1), (1)
Application Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(764), (341), (322), (302), (291), (281), (257), (181), (153), (67), (42), (11), (10), (10), (8), (8), (5), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-BCL2 Antibody

Target Details BCL2 Application Details Handling References for anti-BCL2 Antibody (ABIN1531787) Images
Specificity BCL-2 (Phospho-Thr56) Antibody detects endogenous levels of BCL-2 only when phosphorylated at Thr56.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human BCL-2 around the phosphorylation site of Thr56.
Isotype IgG

Target Details BCL2

Product Details anti-BCL2 Antibody Application Details Handling References for anti-BCL2 Antibody (ABIN1531787) Images back to top
Antigen
Alternative Name BCL-2 (BCL2 Antibody Abstract)
Background Molecular weight of target protein: 26 kDa
Synonyms: Apoptosis regulator Bcl-2, B-cell CLL/lymphoma 2, BCL2
Molecular Weight 26 kDa
Gene ID 10000
OMIM 151430
UniProt P10415
Pathways MAPK Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-BCL2 Antibody Target Details BCL2 Handling References for anti-BCL2 Antibody (ABIN1531787) Images back to top
Application Notes WB: 1:500-1:1000
IHC: 1:50-1:100
IF: 1:100-1:500
ELISA: 1:20000
Optimal working dilution should be determined by the investigator.
Comment

Unigene-Number: Hs.150749 (NCBI Gene Symbol: BCL2)

Restrictions For Research Use only

Handling

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details References for anti-BCL2 Antibody (ABIN1531787) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 52 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20 °C for at least 1 year.
Expiry Date 12 months

References for anti-BCL2 Antibody (ABIN1531787)

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details Handling Images back to top
Background publications

Cleary, Smith, Sklar: "Cloning and structural analysis of cDNAs for bcl-2 and a hybrid bcl-2/immunoglobulin transcript resulting from the t(14;18) translocation." in: Cell, Vol. 47, Issue 1, pp. 19-28, 1986

Images

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details Handling References for anti-BCL2 Antibody (ABIN1531787) back to top
Supplier Images
Western Blotting (WB) image for anti-BCL2 antibody (B-Cell CLL/lymphoma 2) (pThr56) (ABIN1531787) Western blot analysis of extracts from K562 cells treated with H2O2 100uM 30', using ...
Immunohistochemistry (IHC) image for anti-BCL2 antibody (B-Cell CLL/lymphoma 2) (pThr56) (ABIN1531787) Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using BCL-...
Immunofluorescence (IF) image for anti-BCL2 antibody (B-Cell CLL/lymphoma 2) (pThr56) (ABIN1531787) Immunofluorescence analysis of A549 cells, using BCL-2 (Phospho-Thr56) Antibody. The ...