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anti-Human BCL2 antibody for Immunoprecipitation

Recommended BCL2 Antibody (supplied by: Log in to see )

Antigen
B-Cell CLL/lymphoma 2 (BCL2) Antibodies
  • AW986256
  • bcl-2
  • BCL-2
  • Bcl-2
  • Bcl2
  • C430015F12Rik
  • D630044D05Rik
  • D830018M01Rik
  • PPP1R50
Epitope
BH3 Domain
165
72
60
55
50
44
24
24
22
19
17
15
14
14
14
14
14
13
12
11
7
4
4
4
3
3
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
958
225
167
40
22
16
15
7
4
3
2
2
1
1
Host
Rabbit
518
510
19
6
2
2
2
Clonality
Polyclonal
Conjugate
This BCL2 antibody is un-conjugated
46
46
40
37
24
23
19
14
14
13
13
13
13
13
13
13
12
12
12
12
6
6
6
6
6
6
6
2
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
760
340
317
293
290
280
251
176
146
74
42
12
11
10
9
5
3
1
1
1
1
1
Supplier
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Catalog No. ABIN356812
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008946842 ABIN118990 FACS IHC (fro) IHC (p) IP WB Mouse IgG1 AA 41-54 Log in to see 100 4
0.0008946842 ABIN222888 IP IHC WB Rabbit IgG N-Term Log in to see Polyclonal 4
0.0008946842 ABIN967317 IP WB Mouse IgG1 AA 61-76 Log in to see 4D7 10
0.0008946842 ABIN967318 IP WB Mouse IgG1 AA 61-76 Log in to see 4D7 10
0.0008946842 ABIN968024 IF IP IHC WB Mouse IgG1 AA 49-179 Log in to see 7-Bcl 5
0.0008946842 ABIN968025 IF IP IHC WB Mouse IgG1 AA 49-179 Log in to see 7-Bcl 5
0.0008946842 ABIN967312 IHC (fro) FACS IF IP WB Armenian Hamster IgG Log in to see 6C8 13
0.0008946842 ABIN967311 IHC (fro) FACS IF IP WB Hamster IgG Log in to see 6C8 13
0.0008946842 ABIN2657117 FACS IP Alexa Fluor 647 Mouse IgG1 AA 41-54 Log in to see 100 7
0.0008946842 ABIN781707 IHC (fro) FACS IF IHC (p) IP WB Mouse IgG1 AA 41-54 Log in to see Bcl2-100
0.0008946842 ABIN222984 IP IHC WB Mouse IgG1 Log in to see Bcl-2-100 6
0.0008946842 ABIN2664061 FACS IP Mouse IgG1 AA 41-54 Log in to see 100 7
0.0008946842 ABIN638444 FACS ICC IHC (f) IHC (p) IP WB PE Mouse IgG1 AA 41-54 Log in to see Bcl-2-100 5
0.0008946842 ABIN126981 IHC (fro) IP WB Rabbit N-Term Log in to see Polyclonal
0.0008946842 ABIN317971 IHC (p) IP WB Rabbit pSer70 Log in to see Polyclonal
0.0008946842 ABIN317972 IF IHC (p) IP WB Rabbit pThr56 Log in to see Polyclonal
0.0008946842 ABIN957155 IHC (fro) IHC (p) IP WB Rabbit AA 41-54 Log in to see Polyclonal
0.0008946842 ABIN2862600 IF IP IHC ELISA WB Rabbit IgG pThr56 Log in to see Polyclonal
0.0008946842 ABIN2862602 IP IHC ELISA WB Rabbit IgG pSer70 Log in to see Polyclonal
0.0008946842 ABIN1981878 IHC (f) ICC FACS IP IHC (p) WB APC Mouse IgG1 AA 41-54 Log in to see Bcl-2-100 3

Top referenced anti-BCL2 antibody for Immunoprecipitation

Similar anti-BCL2 Antibodies

Application / Reactivity Human
Cellular Assay (CA) 1 Antibodies
Dot Blot (DB) 42 Antibodies
ELISA 310 Antibodies
Enzyme Immunoassay (EIA) 9 Antibodies
Flow Cytometry (FACS) 275 Antibodies
Immunoassay (IA) 1 Antibodies
Immunochromatography (IC) 10 Antibodies
Immunocytochemistry (ICC) 162 Antibodies
Immunofluorescence (IF) 274 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 62 Antibodies
Immunohistochemistry (IHC) 272 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 11 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 143 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 310 Antibodies
Immunoprecipitation (IP) 228 Antibodies
Immunostaining (ISt) 1 Antibodies
Intracellular Staining (ICS) 3 Antibodies

General

Antigen B-Cell CLL/lymphoma 2 (BCL2) Antibodies
Epitope BH3 Domain
(165), (72), (60), (55), (50), (44), (24), (24), (22), (19), (17), (15), (14), (14), (14), (14), (14), (13), (12), (11), (7), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(958), (225), (167), (40), (22), (16), (15), (7), (4), (3), (2), (2), (1), (1)
Host Rabbit
(518), (510), (19), (6), (2), (2), (2)
Clonality
Polyclonal
Conjugate This BCL2 antibody is un-conjugated
(46), (46), (40), (37), (24), (23), (19), (14), (14), (13), (13), (13), (13), (13), (13), (13), (12), (12), (12), (12), (6), (6), (6), (6), (6), (6), (6), (2), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(760), (340), (317), (293), (290), (280), (251), (176), (146), (74), (42), (12), (11), (10), (9), (5), (3), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-BCL2 Antibody

Target Details BCL2 Application Details Handling References for anti-BCL2 Antibody (ABIN356812) Images
Specificity This antibody is specific to Bcl-2 (BH3 Domain).
Purification Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the BH3 domain of human Bcl2.
Isotype Ig

Target Details BCL2

Product Details anti-BCL2 Antibody Application Details Handling References for anti-BCL2 Antibody (ABIN356812) Images back to top
Antigen
Alternative Name Bcl-2 (BCL2 Antibody Abstract)
Background BCL2 is integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma.Synonyms: Apoptosis regulator Bcl-2, B-Cell CLL/lymphoma 2, BCL2, Bcl-2 alpha
Gene ID 596, 9606
UniProt P10415
Research Area Cancer, Apoptosis/Necrosis
Pathways MAPK Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-BCL2 Antibody Target Details BCL2 Handling References for anti-BCL2 Antibody (ABIN356812) Images back to top
Application Notes ELISA: 1/1,000. Western blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100. Immunoprecipitation: 1/100-1/500Flow Cytometry: 1/100-1/500. Immunofluorescence.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details References for anti-BCL2 Antibody (ABIN356812) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS containing 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-BCL2 Antibody (ABIN356812)

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details Handling Images back to top
Background publications

Liu, Zhou, Li, Sun, Lin, Lang, He, Cao, Yan, Wang, Lu, Han, Cao, Zhang, Zeng: "Modulation of orphan nuclear receptor Nur77-mediated apoptotic pathway by acetylshikonin and analogues." in: Cancer research, Vol. 68, Issue 21, pp. 8871-80, 2008

Images

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details Handling References for anti-BCL2 Antibody (ABIN356812) back to top
Supplier Images
 image for anti-BCL2 antibody (B-Cell CLL/lymphoma 2) (BH3 Domain) (ABIN356812) anti-B-Cell CLL/lymphoma 2 (BCL2) (BH3 Domain) antibody
 image for anti-BCL2 antibody (B-Cell CLL/lymphoma 2) (BH3 Domain) (ABIN356812) anti-B-Cell CLL/lymphoma 2 (BCL2) (BH3 Domain) antibody (Image 2)