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anti-Human BCL2 antibody for Immunoprecipitation

Recommended BCL2 Antibody (supplied by: Log in to see )

Antigen
B-Cell CLL/lymphoma 2 (BCL2) Antibodies
  • AW986256
  • Bcl-2
  • C430015F12Rik
  • D630044D05Rik
  • D830018M01Rik
  • PPP1R50
  • BCL-2
  • bcl-2
  • Bcl2
  • B-cell CLL/lymphoma 2
  • B cell leukemia/lymphoma 2
  • B-cell CLL/lymphoma 2 protein
  • B-cell leukemia/lymphoma 2
  • BCL2
  • LOC100224888
  • Bcl2
  • BCL-2
  • bcl2
Epitope
AA 41-54
165
74
58
57
44
33
25
25
24
18
15
15
15
13
13
13
12
12
11
9
7
5
5
5
5
4
4
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
992
259
211
54
28
22
19
18
5
4
4
2
2
2
2
1
Host
Mouse
547
512
20
6
2
2
2
Clonality
Monoclonal
Conjugate
This BCL2 antibody is un-conjugated
47
47
41
38
24
23
20
15
13
13
13
13
13
13
13
13
12
12
12
7
7
7
6
6
6
6
6
6
6
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
798
381
357
314
301
300
227
187
168
81
42
13
12
12
11
9
9
5
3
1
1
1
1
1
Supplier
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Catalog No. ABIN118990
$ 508.20
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN356812 EIA IHC (p) IP WB Rabbit Ig BH3 Domain Log in to see Polyclonal 1
1 ABIN222888 IP IHC WB Rabbit IgG N-Term Log in to see Polyclonal 4
1 ABIN968024 IF IP IHC WB Mouse IgG1 AA 49-179 Log in to see 7-Bcl 5
1 ABIN968025 IF IP IHC WB Mouse IgG1 AA 49-179 Log in to see 7-Bcl 5
1 ABIN967317 IP WB Mouse IgG1 AA 61-76 Log in to see 4D7 10
1 ABIN967318 IP WB Mouse IgG1 AA 61-76 Log in to see 4D7 10
1 ABIN967312 IHC (fro) FACS IF IP WB Armenian Hamster IgG Log in to see 6C8 13
1 ABIN2657117 FACS IP Alexa Fluor 647 Mouse IgG1 AA 41-54 Log in to see 100 7
1 ABIN967311 IHC (fro) FACS IF IP WB Hamster IgG Log in to see 6C8 13
1 ABIN4283360 IHC IHC (p) IP WB Rabbit Log in to see Polyclonal 6
1 ABIN2664061 FACS IP Mouse IgG1 AA 41-54 Log in to see 100 7
1 ABIN4283367 IHC IHC (fro) IHC (p) IP WB Rabbit Log in to see Polyclonal 1
1 ABIN781707 IHC (fro) FACS IF IHC (p) IP WB Mouse IgG1 AA 41-54 Log in to see Bcl2-100
1 ABIN3181931 ELISA IF IHC IP WB Rabbit IgG pThr56 Log in to see Polyclonal
1 ABIN3181930 ELISA IHC IP WB Rabbit IgG pSer70 Log in to see Polyclonal
1 ABIN222984 IP IHC WB Mouse IgG1 Log in to see Bcl-2-100 6
1 ABIN4283369 IHC IHC (fro) IHC (p) IP WB Rabbit Log in to see Polyclonal 2
1 ABIN638444 FACS ICC IHC (f) IHC (p) IP WB PE Mouse IgG1 AA 41-54 Log in to see Bcl-2-100 5
1 ABIN1981878 IHC (f) ICC FACS IP IHC (p) WB APC Mouse IgG1 AA 41-54 Log in to see Bcl-2-100 3
1 ABIN2862600 IF IP IHC ELISA WB Rabbit IgG pThr56 Log in to see Polyclonal

Top referenced anti-BCL2 antibody for Immunoprecipitation

Similar anti-BCL2 Antibodies

Application / Reactivity Human
Cellular Assay (CA) 1 Antibodies
Cytometry by Time of Flight (CyTOF) 12 Antibodies
Dot Blot (DB) 42 Antibodies
ELISA 350 Antibodies
Enzyme Immunoassay (EIA) 8 Antibodies
Flow Cytometry (FACS) 286 Antibodies
Immunoassay (IA) 1 Antibodies
Immunochromatography (IC) 9 Antibodies
Immunocytochemistry (ICC) 173 Antibodies
Immunofluorescence (IF) 298 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 12 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 68 Antibodies
Immunohistochemistry (IHC) 289 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 11 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 166 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 350 Antibodies
Immunoprecipitation (IP) 213 Antibodies

General

Antigen B-Cell CLL/lymphoma 2 (BCL2) Antibodies
Epitope AA 41-54
(165), (74), (58), (57), (44), (33), (25), (25), (24), (18), (15), (15), (15), (13), (13), (13), (12), (12), (11), (9), (7), (5), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(992), (259), (211), (54), (28), (22), (19), (18), (5), (4), (4), (2), (2), (2), (2), (1)
Host Mouse
(547), (512), (20), (6), (2), (2), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This BCL2 antibody is un-conjugated
(47), (47), (41), (38), (24), (23), (20), (15), (13), (13), (13), (13), (13), (13), (13), (13), (12), (12), (12), (7), (7), (7), (6), (6), (6), (6), (6), (6), (6), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(798), (381), (357), (314), (301), (300), (227), (187), (168), (81), (42), (13), (12), (12), (11), (9), (9), (5), (3), (1), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-BCL2 Antibody

Target Details BCL2 Application Details Handling References for anti-BCL2 Antibody (ABIN118990) Images
Specificity This antibody clone 100 reacts specifically with the 25 kDa Bcl-2 oncoprotein. This antibody reacts with small B lymphocytes in the mantle zone and many cells within T cell areas. Very few cells in germinal centres are stained. In the thymus many cells in the medulla are stained but the cortex shows weak or negative staining. In non-haematopoietic tissues few cells are stained. The antibody reacts with neoplastic cells of follicular lymphoma and anaplastic large cell lymphoma.
Purification Affinity chromatography on Protein G
Immunogen Synthetic peptide containing aa 41-54 of the bcl-2 protein.
Clone 100
Isotype IgG1

Target Details BCL2

Product Details anti-BCL2 Antibody Application Details Handling References for anti-BCL2 Antibody (ABIN118990) Images back to top
Antigen
Alternative Name Bcl-2 (BCL2 Antibody Abstract)
Background Human Apoptosis regulator Bcl-2 oncoprotein, a 239 amino acid ~25 kDa integral single pass membrane protein containing 4 BH motifs which lies within the cell rather than on the cell surface. The protein is localised in the outer mitochondrial membrane and plays a role in the inhibition of apoptosis. Bcl-2 is an integral membrane protein which lies within the cell rather than on the cell surface. The protein is localised in mitochondria and plays a role in the inhibition of apoptosis.Synonyms: Apoptosis regulator Bcl-2, B-Cell CLL/lymphoma 2, BCL2, Bcl-2 alpha
Molecular Weight 25-26 kDa
Gene ID 596
NCBI Accession NP_000624
UniProt P10415
Research Area Cancer, Apoptosis/Necrosis
Pathways MAPK Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization

Application Details

Product Details anti-BCL2 Antibody Target Details BCL2 Handling References for anti-BCL2 Antibody (ABIN118990) Images back to top
Application Notes Immunoprecipitation. Western Blot. Flow Cytometry (1/50-1/100): Membrane permeabilisation is required for this application. Immunohistochemistry on Frozen Sections: (1/20-1/40). Immunohistochemistry on Paraffin Sections: (1/20-1/40): This product requires antigen retrieval using heat treatment prior to staining of formalin fixed material Fixation using BS or Bouins fluid is preferable to formalin fixation. Recommended Positive Control: Human tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details References for anti-BCL2 Antibody (ABIN118990) Images back to top
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4, 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-BCL2 Antibody (ABIN118990)

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details Handling Images back to top
Background publications

Hockenbery, Nuñez, Milliman, Schreiber, Korsmeyer: "Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death." in: Nature, Vol. 348, Issue 6299, pp. 334-6, 1991

Pezzella, Tse, Cordell, Pulford, Gatter, Mason: "Expression of the bcl-2 oncogene protein is not specific for the 14;18 chromosomal translocation." in: The American journal of pathology, Vol. 137, Issue 2, pp. 225-32, 1990

Tsujimoto, Croce: "Analysis of the structure, transcripts, and protein products of bcl-2, the gene involved in human follicular lymphoma." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, Issue 14, pp. 5214-8, 1986

Cleary, Smith, Sklar: "Cloning and structural analysis of cDNAs for bcl-2 and a hybrid bcl-2/immunoglobulin transcript resulting from the t(14;18) translocation." in: Cell, Vol. 47, Issue 1, pp. 19-28, 1986