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anti-Human BCL2 antibody for Western Blotting

Recommended BCL2 Antibody (supplied by: Log in to see )

Antigen
B-Cell CLL/lymphoma 2 (BCL2) Antibodies
  • AW986256
  • bcl-2
  • BCL-2
  • Bcl-2
  • Bcl2
  • C430015F12Rik
  • D630044D05Rik
  • D830018M01Rik
  • PPP1R50
Epitope
AA 41-54
75
71
53
50
48
44
23
23
19
17
15
14
14
13
13
13
13
12
12
9
7
4
4
4
3
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
673
200
151
17
9
7
4
3
2
2
1
1
Host
Mouse
494
239
19
5
2
2
2
Clonality
Monoclonal
Conjugate
This BCL2 antibody is un-conjugated
38
33
28
24
19
14
11
10
7
6
6
6
6
6
6
3
1
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
480
275
241
144
138
138
112
67
49
42
32
11
10
10
8
8
5
1
1
1
1
1
Supplier
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Catalog No. ABIN1105504
$ 313.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.12227175 ABIN657744 WB Rabbit Ig AA 86-114, Center Log in to see Polyclonal 1
0.12227175 ABIN1105506 FACS WB Mouse IgG1 Log in to see 83-8B 2
0.12227175 ABIN222888 IP IHC WB Rabbit IgG N-Term Log in to see Polyclonal 4
0.12227175 ABIN388095 IF IHC (p) WB Rabbit Ig AA 75-110, BH3 Domain Log in to see Polyclonal 19
0.12227175 ABIN185269 ELISA WB Goat N-Term Log in to see Polyclonal 1
0.12227175 ABIN2792106 IHC WB Rabbit N-Term Log in to see Polyclonal 1
0.12227175 ABIN1531786 IHC ELISA WB Rabbit IgG pSer70 Log in to see Polyclonal 1
0.12227175 ABIN1531787 IF IHC ELISA WB Rabbit IgG pThr56 Log in to see Polyclonal 1
0.12227175 ABIN1531302 IHC ELISA WB Rabbit IgG pSer87 Log in to see Polyclonal 1
0.12227175 ABIN1532816 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 1
0.12227175 ABIN499427 EIA IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal 1
0.12227175 ABIN499429 EIA IF WB Rabbit IgG Intermediate Domain Log in to see Polyclonal 1
0.12227175 ABIN968024 IF IP IHC WB Mouse IgG1 AA 49-179 Log in to see 7-Bcl 5
0.12227175 ABIN968025 IF IP IHC WB Mouse IgG1 AA 49-179 Log in to see 7-Bcl 5
0.12227175 ABIN967317 IP WB Mouse IgG1 AA 61-76 Log in to see 4D7 10
0.12227175 ABIN967318 IP WB Mouse IgG1 AA 61-76 Log in to see 4D7 10
0.12227175 ABIN1881107 WB Rabbit AA 144-171, Center Log in to see Polyclonal 1
0.12227175 ABIN1881108 WB Rabbit AA 87-116, Center Log in to see Polyclonal 1
0.12227175 ABIN967312 IHC (fro) FACS IF IP WB Armenian Hamster IgG Log in to see 6C8 13
0.12227175 ABIN2787860 WB Rabbit Middle Region Log in to see Polyclonal

Top referenced anti-BCL2 antibody for Western Blotting

Similar anti-BCL2 Antibodies

Application / Reactivity Human
Cellular Assay (CA) 1 Antibodies
Dot Blot (DB) 42 Antibodies
ELISA 246 Antibodies
Enzyme Immunoassay (EIA) 9 Antibodies
Flow Cytometry (FACS) 116 Antibodies
Immunoassay (IA) 1 Antibodies
Immunochromatography (IC) 10 Antibodies
Immunocytochemistry (ICC) 51 Antibodies
Immunofluorescence (IF) 146 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 56 Antibodies
Immunohistochemistry (IHC) 240 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 8 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 31 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 112 Antibodies
Immunoprecipitation (IP) 132 Antibodies
Immunostaining (ISt) 1 Antibodies
Intracellular Staining (ICS) 3 Antibodies

General

Antigen B-Cell CLL/lymphoma 2 (BCL2) Antibodies
Epitope AA 41-54
(75), (71), (53), (50), (48), (44), (23), (23), (19), (17), (15), (14), (14), (13), (13), (13), (13), (12), (12), (9), (7), (4), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(673), (200), (151), (17), (9), (7), (4), (3), (2), (2), (1), (1)
Host Mouse
(494), (239), (19), (5), (2), (2), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This BCL2 antibody is un-conjugated
(38), (33), (28), (24), (19), (14), (11), (10), (7), (6), (6), (6), (6), (6), (6), (3), (1), (1), (1), (1)
Application Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(480), (275), (241), (144), (138), (138), (112), (67), (49), (42), (32), (11), (10), (10), (8), (8), (5), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-BCL2 Antibody

Target Details BCL2 Application Details Handling References for anti-BCL2 Antibody (ABIN1105504) Images
Specificity This antibody reacts to BCL2.
Purification Affinity chromatography
Immunogen Synthetic peptide corresponding to residues 41-54pf the bcl-2 protein, conjugated to thyroglobulin.
Clone BL-2
Isotype IgG1

Target Details BCL2

Product Details anti-BCL2 Antibody Application Details Handling References for anti-BCL2 Antibody (ABIN1105504) Images back to top
Antigen
Alternative Name Bcl-2 (BCL2 Antibody Abstract)
Background Immunoreactive BCL2 protein in the neoplastic cells of almost all follicular lymphomas whereas no BCL2 protein was detected in follicles affected by nonneoplastic processes or in normal lymphoid tissue. Every tumor with molecular-genetic evidence of t(14,18) translocation expressed detectable levels of BCL2 protein, regardless of whether the breakpoint was located in or at a distance from the BCL2 gene. Overexpression of BCL2 blocks the apoptotic death of a pro-B-lymphocyte cell line.Synonyms: Apoptosis regulator Bcl-2, B-Cell CLL/lymphoma 2, BCL2, Bcl-2 alpha
Gene ID 596
NCBI Accession NP_000624
UniProt P10415
Research Area Cancer, Apoptosis/Necrosis
Pathways MAPK Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-BCL2 Antibody Target Details BCL2 Handling References for anti-BCL2 Antibody (ABIN1105504) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details References for anti-BCL2 Antibody (ABIN1105504) Images back to top
Reconstitution Restore with 1.2 % sodium acetate or neutral PBS
Concentration 0,1 mg/mL
Buffer 1.2 % sodium acetate, with 2 mg BSA and 0.01 mg sodium azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Prior to reconstitution store at -20 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-BCL2 Antibody (ABIN1105504)

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details Handling Images back to top
Background publications

Hockenbery, Nuñez, Milliman, Schreiber, Korsmeyer: "Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death." in: Nature, Vol. 348, Issue 6299, pp. 334-6, 1991

Ngan, Chen-Levy, Weiss, Warnke, Cleary: "Expression in non-Hodgkin's lymphoma of the bcl-2 protein associated with the t(14;18) chromosomal translocation." in: The New England journal of medicine, Vol. 318, Issue 25, pp. 1638-44, 1988

Images

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details Handling References for anti-BCL2 Antibody (ABIN1105504) back to top
Supplier Images
Western Blotting (WB) image for anti-BCL2 antibody (B-Cell CLL/lymphoma 2) (AA 41-54) (ABIN1105504) anti-B-Cell CLL/lymphoma 2 (BCL2) (AA 41-54) antibody