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anti-Monkey BCL2 antibody for Flow Cytometry

Recommended BCL2 Antibody (supplied by: Log in to see )

Antigen
B-Cell CLL/lymphoma 2 (BCL2) Antibodies
  • AW986256
  • Bcl-2
  • C430015F12Rik
  • D630044D05Rik
  • D830018M01Rik
  • PPP1R50
  • BCL-2
  • bcl-2
  • Bcl2
  • B-cell CLL/lymphoma 2
  • B cell leukemia/lymphoma 2
  • B-cell CLL/lymphoma 2 protein
  • B-cell leukemia/lymphoma 2
  • BCL2
  • LOC100224888
  • Bcl2
  • BCL-2
  • bcl2
Epitope
AA 41-54
164
73
64
59
41
33
25
25
24
18
15
15
15
14
13
13
13
11
8
7
6
5
5
5
5
5
4
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Monkey, Pig (Porcine)
988
251
211
53
28
22
18
17
5
4
4
3
2
2
2
2
Host
Mouse
539
510
19
6
2
2
2
Clonality
Monoclonal
Conjugate
This BCL2 antibody is un-conjugated
47
46
42
37
24
23
21
15
14
13
13
13
13
13
13
13
12
12
12
7
7
7
6
6
6
6
6
6
6
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
781
368
359
306
304
288
226
185
169
81
40
13
13
12
12
11
9
4
3
1
1
1
1
1
1
1
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Catalog No. ABIN3026592
$ 361.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN386113 FACS IF IHC (p) WB Mouse IgG1 AA 41-54 Log in to see 8C8
1 ABIN337080 FACS IF IHC (p) ELISA WB Mouse IgG1 AA 41-54 Log in to see
1 ABIN386116 FACS IF IHC (p) WB Biotin Mouse IgG1 AA 41-54 Log in to see 8C8
1 ABIN386117 FACS IF IHC (p) WB Biotin Mouse IgG1 AA 41-54 Log in to see 8C8
1 ABIN2738523 ELISA FACS IF IHC (p) IP WB Mouse IgG1, kappa AA 41-54 Log in to see 8C8
1 ABIN386114 FACS IF IHC (p) WB Mouse IgG1 AA 41-54 Log in to see 8C8
1 ABIN3026594 FACS IF IHC WB Mouse IgG1, kappa AA 41-54 Log in to see 8C8

Similar anti-BCL2 Antibodies

Application / Reactivity Pig (Porcine) Monkey Human
ELISA 23 Antibodies 7 Antibodies
Flow Cytometry (FACS) 30 Antibodies 8 Antibodies
Immunocytochemistry (ICC) 21 Antibodies
Immunofluorescence (IF) 32 Antibodies 8 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
Immunohistochemistry (IHC) 22 Antibodies 6 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 23 Antibodies 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 36 Antibodies 9 Antibodies
Immunoprecipitation (IP) 24 Antibodies 7 Antibodies
Western Blotting (WB) 40 Antibodies 17 Antibodies
Cell Culture (CC) 1 Antibodies

General

Antigen B-Cell CLL/lymphoma 2 (BCL2) Antibodies
Epitope AA 41-54
(164), (73), (64), (59), (41), (33), (25), (25), (24), (18), (15), (15), (15), (14), (13), (13), (13), (11), (8), (7), (6), (5), (5), (5), (5), (5), (4), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Monkey, Pig (Porcine)
(988), (251), (211), (53), (28), (22), (18), (17), (5), (4), (4), (3), (2), (2), (2), (2)
Host Mouse
(539), (510), (19), (6), (2), (2), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This BCL2 antibody is un-conjugated
(47), (46), (42), (37), (24), (23), (21), (15), (14), (13), (13), (13), (13), (13), (13), (13), (12), (12), (12), (7), (7), (7), (6), (6), (6), (6), (6), (6), (6), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(781), (368), (359), (306), (304), (288), (226), (185), (169), (81), (40), (13), (13), (12), (12), (11), (9), (4), (3), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-BCL2 Antibody

Target Details BCL2 Application Details Handling Images
No Cross-Reactivity Mouse (Murine), Rat (Rattus)
Characteristics This antibody recognizes a protein of 25-26 kDa, identified as the Bcl-2 alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of Bcl-2 alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of Bcl-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express Bcl-2 protein and the small number in which the neoplastic cells are Bcl-2 negative.
Purification Protein G affinity chromatography
Immunogen A synthetic peptide, aa 41-54 (GAAPAPGIFSSQPG-Cys) of human Bcl-2 protein was used as the immunogen for this antibody.
Clone 8C8
Isotype IgG1, kappa

Target Details BCL2

Product Details anti-BCL2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Bcl-2 (BCL2 Antibody Abstract)
Background This antibody recognizes a protein of 25-26 kDa, identified as the Bcl-2 alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of Bcl-2 alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of Bcl-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express Bcl-2 protein and the small number in which the neoplastic cells are Bcl-2 negative.
Gene ID 596
Research Area Cancer, Apoptosis/Necrosis
Pathways MAPK Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling

Application Details

Product Details anti-BCL2 Antibody Target Details BCL2 Handling Images back to top
Application Notes The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 1 mM EDTA Buffer, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,WB: 0.5-1 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions For Research Use only

Handling

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details Images back to top
Concentration 0.2 mg/mL
Buffer 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C,4 °C
Storage Comment Store the Bcl-2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Images

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-BCL2 antibody (B-Cell CLL/lymphoma 2) (AA 41-54) (ABIN3026592) IHC testing of human tonsil stained with Bcl-2 antibody (8C8).Note cell membrane and ...
Western Blotting (WB) image for anti-BCL2 antibody (B-Cell CLL/lymphoma 2) (AA 41-54) (ABIN3026592) Western blot testing of human samples with Bcl-2 antibody (8C8).