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anti-Pig (Porcine) BCL2 antibody for Western Blotting

Recommended BCL2 Antibody (supplied by: Log in to see )

Antigen
B-Cell CLL/lymphoma 2 (BCL2) Antibodies
  • AW986256
  • bcl-2
  • BCL-2
  • Bcl-2
  • Bcl2
  • C430015F12Rik
  • D630044D05Rik
  • D830018M01Rik
  • PPP1R50
Epitope
AA 41-54
164
72
60
55
50
44
24
24
22
19
17
15
14
14
14
14
14
13
12
12
7
4
4
4
3
3
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Monkey, Pig (Porcine)
958
225
167
39
22
16
15
6
4
3
2
2
1
1
Host
Mouse
519
509
19
6
2
2
2
Clonality
Monoclonal
Conjugate
This BCL2 antibody is un-conjugated
46
46
40
37
24
23
19
14
14
13
13
13
13
13
13
13
12
12
12
12
6
6
6
6
6
6
6
2
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
760
340
318
292
289
279
252
176
146
74
42
12
11
10
10
5
3
1
1
1
1
1
Supplier
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Catalog No. ABIN3026592
$ 361.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.124960825 ABIN4283388 FACS ICC IF IHC IHC (fro) IHC (p) WB Mouse IgG1 kappa Log in to see 8C8
0.124960825 ABIN4283394 ELISA ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 kappa Log in to see 8C8
0.124960825 ABIN4283406 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB HRP Mouse IgG1 kappa Log in to see 8C8
0.124960825 ABIN2155385 WB Goat Log in to see Polyclonal
0.0012207722 ABIN707156 IF (p) IHC (p) WB Rabbit IgG AA 169-211 Log in to see Polyclonal 5
0.0012207722 ABIN707165 IHC (p) WB HRP Rabbit IgG AA 169-211 Log in to see Polyclonal
0.0012207722 ABIN4283401 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB Allophycocyanin Mouse IgG1 kappa Log in to see 8C8
0.0012207722 ABIN4283399 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB Alexa Fluor 647 Mouse IgG1 kappa Log in to see 8C8
0.0012207722 ABIN4283409 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB PE Mouse IgG1 kappa Log in to see 8C8
0.0012207722 ABIN4283405 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB DyLight 488 Mouse IgG1 kappa Log in to see 8C8
0.0012207722 ABIN4283397 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB Alexa Fluor 405 Mouse IgG1 kappa Log in to see 8C8
0.0012207722 ABIN4283407 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB DyLight 755 Mouse IgG1 kappa Log in to see 8C8
0.0012207722 ABIN4283398 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB Alexa Fluor 488 Mouse IgG1 kappa Log in to see 8C8
0.0012207722 ABIN4283402 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB Biotin Mouse IgG1 kappa Log in to see 8C8
0.0012207722 ABIN4283411 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB DyLight 350 Mouse IgG1 kappa Log in to see 8C8
0.0012207722 ABIN4283404 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB DyLight 680 Mouse IgG1 kappa Log in to see 8C8
0.0012207722 ABIN4283408 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB PerCP Mouse IgG1 kappa Log in to see 8C8
0.0012207722 ABIN4283410 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB DyLight 550 Mouse IgG1 kappa Log in to see 8C8
0.0012207722 ABIN4283400 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB Alexa Fluor 700 Mouse IgG1 kappa Log in to see 8C8
0.0012207722 ABIN4283403 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB DyLight 650 Mouse IgG1 kappa Log in to see 8C8

Top referenced anti-BCL2 antibody for Western Blotting

Similar anti-BCL2 Antibodies

Application / Reactivity Pig (Porcine) Monkey Human
ELISA 18 Antibodies 3 Antibodies
Flow Cytometry (FACS) 21 Antibodies 2 Antibodies
Immunocytochemistry (ICC) 21 Antibodies
Immunofluorescence (IF) 23 Antibodies 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 12 Antibodies
Immunohistochemistry (IHC) 22 Antibodies 6 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 21 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 25 Antibodies
Immunoprecipitation (IP) 18 Antibodies 3 Antibodies
Western Blotting (WB) 27 Antibodies 6 Antibodies
Cellular Assay (CA) 1 Antibodies
Dot Blot (DB) 42 Antibodies

General

Antigen B-Cell CLL/lymphoma 2 (BCL2) Antibodies
Epitope AA 41-54
(164), (72), (60), (55), (50), (44), (24), (24), (22), (19), (17), (15), (14), (14), (14), (14), (14), (13), (12), (12), (7), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Monkey, Pig (Porcine)
(958), (225), (167), (39), (22), (16), (15), (6), (4), (3), (2), (2), (1), (1)
Host Mouse
(519), (509), (19), (6), (2), (2), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This BCL2 antibody is un-conjugated
(46), (46), (40), (37), (24), (23), (19), (14), (14), (13), (13), (13), (13), (13), (13), (13), (12), (12), (12), (12), (6), (6), (6), (6), (6), (6), (6), (2), (1), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(760), (340), (318), (292), (289), (279), (252), (176), (146), (74), (42), (12), (11), (10), (10), (5), (3), (1), (1), (1), (1), (1)
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Product Details anti-BCL2 Antibody

Target Details BCL2 Application Details Handling Images
No Cross-Reactivity Mouse (Murine), Rat (Rattus)
Characteristics This antibody recognizes a protein of 25-26 kDa, identified as the Bcl-2 alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of Bcl-2 alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of Bcl-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express Bcl-2 protein and the small number in which the neoplastic cells are Bcl-2 negative.
Purity Protein G affinity chromatography
Immunogen A synthetic peptide, aa 41-54 (GAAPAPGIFSSQPG-Cys) of human Bcl-2 protein was used as the immunogen for this antibody.
Clone 8C8
Isotype IgG1, kappa

Target Details BCL2

Product Details anti-BCL2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Bcl-2 (BCL2 Antibody Abstract)
Gene ID 596
Research Area Cancer, Apoptosis/Necrosis
Pathways MAPK Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-BCL2 Antibody Target Details BCL2 Handling Images back to top
Application Notes The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 1 mM EDTA Buffer, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,WB: 0.5-1 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions For Research Use only

Handling

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details Images back to top
Concentration 0.2 mg/mL
Buffer 0.2 mg/mL with 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C,4 °C
Storage Comment Store the Bcl-2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Images

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-BCL2 antibody (B-Cell CLL/lymphoma 2) (AA 41-54) (ABIN3026592) IHC testing of human tonsil stained with Bcl-2 antibody (8C8).Note cell membrane and ...
Western Blotting (WB) image for anti-BCL2 antibody (B-Cell CLL/lymphoma 2) (AA 41-54) (ABIN3026592) Western blot testing of human samples with Bcl-2 antibody (8C8).