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anti-Rat (Rattus) BCL2 antibody for Immunocytochemistry

Recommended BCL2 Antibody (supplied by: Log in to see )

Antigen
B-Cell CLL/lymphoma 2 (BCL2) Antibodies
  • AW986256
  • bcl-2
  • BCL-2
  • Bcl-2
  • Bcl2
  • C430015F12Rik
  • D630044D05Rik
  • D830018M01Rik
  • PPP1R50
Epitope
N-Term, AA 61-76
76
71
53
50
48
44
23
23
19
17
15
14
14
13
13
13
13
12
12
9
7
4
4
4
3
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Mouse (Murine), Rat (Rattus)
674
200
151
17
9
7
4
3
2
2
1
1
Host
Mouse
494
240
19
5
2
2
2
Clonality
Monoclonal
Conjugate
This BCL2 antibody is un-conjugated
38
33
28
24
19
14
11
10
7
6
6
6
6
6
6
3
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
481
275
241
145
138
138
113
67
49
42
33
11
10
10
8
8
5
1
1
1
1
1
Supplier
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Available images

Catalog No. ABIN256543
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010161072 ABIN1846235 ICC IF IHC WB Rabbit Log in to see Polyclonal
0.0010161072 ABIN2988430 ICC IF IP IHC WB Rabbit IgG Log in to see Polyclonal
0.0010161072 ABIN3066908 ICC IF IHC WB Mouse Log in to see
0.0010161072 ABIN2858812 ICC IF IHC WB Rabbit Log in to see Polyclonal

Similar anti-BCL2 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 27 Antibodies 55 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies 7 Antibodies
Flow Cytometry (FACS) 25 Antibodies 32 Antibodies
Functional Studies (Func) 1 Antibodies 1 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 5 Antibodies
Immunofluorescence (IF) 21 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 11 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 45 Antibodies
Immunohistochemistry (IHC) 32 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 18 Antibodies
Immunoprecipitation (IP) 15 Antibodies
Intracellular Staining (ICS) 5 Antibodies
Western Blotting (WB) 82 Antibodies
Fluorescence Microscopy (FM) 1 Antibodies

General

Antigen B-Cell CLL/lymphoma 2 (BCL2) Antibodies
Epitope N-Term, AA 61-76
(76), (71), (53), (50), (48), (44), (23), (23), (19), (17), (15), (14), (14), (13), (13), (13), (13), (12), (12), (9), (7), (4), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine), Rat (Rattus)
(674), (200), (151), (17), (9), (7), (4), (3), (2), (2), (1), (1)
Host Mouse
(494), (240), (19), (5), (2), (2), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This BCL2 antibody is un-conjugated
(38), (33), (28), (24), (19), (14), (11), (10), (7), (6), (6), (6), (6), (6), (6), (3), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(481), (275), (241), (145), (138), (138), (113), (67), (49), (42), (33), (11), (10), (10), (8), (8), (5), (1), (1), (1), (1), (1)
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Product Details anti-BCL2 Antibody

Target Details BCL2 Application Details Handling Images
Purification affinity purified
Immunogen N-terminal, Amino acid residues 61-76 of mouse Bcl-2
Clone BCL-10C4
Isotype IgG1 kappa

Target Details BCL2

Product Details anti-BCL2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Bcl-2 (BCL2 Antibody Abstract)
Background Bcl-2 (B-cell leukemia 2) is an apoptotic protein and a member of the Bcl-2 familycontaining BH1-4 domains. Two reported isoforms exist alpha=25 kD, beta=22 kD.The Bcl-2 protein forms homo- or hetero-dimers with other Bcl-2 family members. Bcl-2is distributed in the outer mitochondrial membrane, intracellular membrane nuclearenvelope, and endoplasmic reticulum. This protein blocks apoptotic death by controllingmitochondrial membrane permeability. Cleavage of Bcl-2 can convert to pro-apoptotic(by cleavage of BH4 domain). Bcl-2 has been reported to regulate cell cycle progressionvia ROS. This protein is modified by ASK1/JNK1, PKC, ERKs, and stress-activatedkinase phosphorylation and can be ubiquitinated. Bcl-2 has been shown to interact withApaf-1, Raf-1, TP53BP2, caspase-3, and form heterodimers with Bax, Bad, Bak, Bcl-xL,and Bag-1. Bcl-2 is modified by phosphorylation and ubiquitination. Clone BCL/10C4has been shown to be useful for Western blotting, immunoprecipitation, andimmunofluorescence of the mouse and rat Bcl-2 protein. Alternate Names: anti-B-cell leukemia 2 antibody.
Gene Symbol: BCL2
Gene ID 596
Research Area Cancer, Apoptosis/Necrosis
Pathways MAPK Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-BCL2 Antibody Target Details BCL2 Handling Images back to top
Application Notes Immunoprecipitation and Immunofluorescence use reported in literature.
Recommended dilutions: Immunoprecipitation 1:10-1:500, Western Blot 1:1000, Immunocytochemistry/Immunofluorescence 1-4 µg/mL
Restrictions For Research Use only

Handling

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details Images back to top
Concentration 0.5 mg/mL
Buffer PBS, pH 7.2, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C

Images

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-BCL2 antibody (B-Cell CLL/lymphoma 2) (N-Term) (ABIN256543) Western blot analysis of extracts from mouse splenocytes using anti-Bcl-2, clone BCL/...