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anti-Rat (Rattus) BCL2 antibody for Immunofluorescence

Recommended BCL2 Antibody (supplied by: Log in to see )

Antigen
B-Cell CLL/lymphoma 2 (BCL2) Antibodies
  • AW986256
  • Bcl-2
  • C430015F12Rik
  • D630044D05Rik
  • D830018M01Rik
  • PPP1R50
  • BCL-2
  • bcl-2
  • Bcl2
  • B-cell CLL/lymphoma 2
  • B cell leukemia/lymphoma 2
  • B-cell CLL/lymphoma 2 protein
  • B-cell leukemia/lymphoma 2
  • BCL2
  • LOC100224888
  • Bcl2
  • BCL-2
  • bcl2
Epitope
AA 145-157, Internal Region
166
74
58
57
44
32
25
25
24
18
15
15
15
13
13
13
12
12
11
9
7
5
5
5
5
4
4
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
992
258
210
54
28
22
19
18
5
4
4
2
2
2
2
1
Host
Rabbit
546
513
20
6
2
2
2
Clonality
Polyclonal
Conjugate
This BCL2 antibody is un-conjugated
47
47
41
38
24
23
20
15
13
13
13
13
13
13
13
13
12
12
12
7
7
7
6
6
6
6
6
6
6
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
798
381
357
313
302
300
228
187
169
81
42
13
12
12
11
9
8
5
3
1
1
1
1
1
Supplier
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Catalog No. ABIN950625
$ 500.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
12.237935 ABIN1532505 IF IHC ELISA WB Rabbit IgG Log in to see Polyclonal
12.237935 ABIN2957874 IC IF IP IHC WB Rabbit pSer56 Log in to see Polyclonal
12.237935 ABIN1871251 IF WB Rabbit IgG Log in to see Polyclonal
12.237935 ABIN1876758 IF IHC IP WB Rabbit IgG Log in to see Polyclonal
12.237935 ABIN2162973 IF IHC ELISA WB Rabbit IgG Thr69 Log in to see Polyclonal
12.237935 ABIN3030102 IF IHC ELISA WB Rabbit Ig Fraction AA 75-110 Log in to see Polyclonal
12.237935 ABIN2852691 IC IF IHC WB Mouse Log in to see
12.237935 ABIN2403428 IF IHC WB Rabbit IgG Log in to see Polyclonal
1 ABIN256543 ICC IF IP WB Mouse IgG1 kappa N-Term, AA 61-76 Log in to see BCL-10C4 2
1 ABIN968024 IF IP IHC WB Mouse IgG1 AA 49-179 Log in to see 7-Bcl 5
1 ABIN968025 IF IP IHC WB Mouse IgG1 AA 49-179 Log in to see 7-Bcl 5
1 ABIN271996 IF IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN3183498 ELISA IF IHC WB Rabbit IgG Ser168, Ser170 Log in to see Polyclonal
1 ABIN1732255 IF IHC (p) ELISA WB Rabbit IgG AA 46-95, Internal Region Log in to see Polyclonal
1 ABIN2878827 IF IHC (p) ELISA WB Rabbit IgG AA 46-95 Log in to see Polyclonal
1 ABIN264903 IF IP WB Sheep IgG Log in to see Polyclonal
1 ABIN1821134 IF IP WB Mouse IgG1, kappa AA 61-76 Log in to see G1-G1-4
1 ABIN4283378 ICC IF WB DyLight 350 Mouse IgG1 kappa AA 61-76, N-Term Log in to see BCL-10C4
1 ABIN4283382 ICC IF IP WB PerCP Mouse IgG1 kappa AA 61-76, N-Term Log in to see BCL-10C4
1 ABIN4283384 ICC IF WB DyLight 680 Mouse IgG1 kappa AA 61-76, N-Term Log in to see BCL-10C4

Top referenced anti-BCL2 antibody for Immunofluorescence

Similar anti-BCL2 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 37 Antibodies 75 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies 6 Antibodies
Flow Cytometry (FACS) 22 Antibodies 34 Antibodies
Functional Studies (Func) 1 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 14 Antibodies
Immunofluorescence (IF) 37 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 13 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 3 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 55 Antibodies
Immunohistochemistry (IHC) 40 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 5 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 29 Antibodies
Immunoprecipitation (IP) 28 Antibodies
Western Blotting (WB) 133 Antibodies
Fluorescence Microscopy (FM) 1 Antibodies

General

Antigen B-Cell CLL/lymphoma 2 (BCL2) Antibodies
Epitope AA 145-157, Internal Region
(166), (74), (58), (57), (44), (32), (25), (25), (24), (18), (15), (15), (15), (13), (13), (13), (12), (12), (11), (9), (7), (5), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(992), (258), (210), (54), (28), (22), (19), (18), (5), (4), (4), (2), (2), (2), (2), (1)
Host Rabbit
(546), (513), (20), (6), (2), (2), (2)
Clonality
Polyclonal
Conjugate This BCL2 antibody is un-conjugated
(47), (47), (41), (38), (24), (23), (20), (15), (13), (13), (13), (13), (13), (13), (13), (13), (12), (12), (12), (7), (7), (7), (6), (6), (6), (6), (6), (6), (6), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(798), (381), (357), (313), (302), (300), (228), (187), (169), (81), (42), (13), (12), (12), (11), (9), (8), (5), (3), (1), (1), (1), (1), (1)
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Product Details anti-BCL2 Antibody

Target Details BCL2 Application Details Handling Images
Specificity This antibody detects endogenous levels of total BCL-2 protein.
Cross-Reactivity (Details) Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
Purification Immunoaffinity Chromatography
Immunogen Synthetic peptide from Human BCL-2. Epitope: Internal
Isotype IgG

Target Details BCL2

Product Details anti-BCL2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Bcl-2 (BCL2 Antibody Abstract)
Background BCL2 is an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants (alpha and beta) produced by alternate splicing, differ in their C-terminal ends. BCL2 suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. It regulates cell death by controlling the mitochondrial membrane permeability. It appears to function in a feedback loop system with caspases. BCL2 inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF1). It can form homodimers, and heterodimers with BAX, BAD, BAK and BclX(L). Heterodimerization with BAX requires intact BH1 and BH2 domains, and is necessary for anti-apoptotic activity. Also interacts with APAF1, RAF1, TP53BP2, BBC3, BCL2L1 and BNIPL.Synonyms: Apoptosis regulator Bcl-2, B-Cell CLL/lymphoma 2, BCL2, Bcl-2 alpha
Gene ID 596
NCBI Accession NP_000624
Research Area Cancer, Apoptosis/Necrosis
Pathways MAPK Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization

Application Details

Product Details anti-BCL2 Antibody Target Details BCL2 Handling Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details Images back to top
Concentration 1.0 mg/mL
Buffer PBS (without Mg2+, Ca2+), pH 7.4, 150 mM Sodium Chloride, 0.02 % Sodium Azide and 50 % Glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Images

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details Handling back to top
Supplier Images
Immunofluorescence (IF) image for anti-BCL2 antibody (B-Cell CLL/lymphoma 2) (AA 145-157) (ABIN950625) Immunofluorescence analysis of HepG2 cells, using BCL2 Antibody . The picture on the ...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-BCL2 antibody (B-Cell CLL/lymphoma 2) (AA 145-157) (ABIN950625) Formalin-Fixed, Paraffin-Embedded Human Kidney, Tubules stained with BCL2
Western Blotting (WB) image for anti-BCL2 antibody (B-Cell CLL/lymphoma 2) (AA 145-157) (ABIN950625) Western blot analysis of extracts from K562 cells, using BCL2 Antibody . The lane on ...