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anti-Rat (Rattus) BCL2 antibody for Immunohistochemistry (Frozen Sections)

Recommended BCL2 Antibody (supplied by: Log in to see )

Antigen
B-Cell CLL/lymphoma 2 (BCL2) Antibodies
  • AW986256
  • bcl-2
  • BCL-2
  • Bcl-2
  • Bcl2
  • C430015F12Rik
  • D630044D05Rik
  • D830018M01Rik
  • PPP1R50
Epitope
N-Term
76
71
53
50
47
44
23
23
19
17
15
14
14
13
13
13
13
12
12
9
7
4
4
4
3
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
711
199
150
17
9
7
4
3
2
2
1
1
Host
Rabbit
493
278
19
5
2
2
2
Clonality
Polyclonal
Conjugate
This BCL2 antibody is un-conjugated
40
35
30
26
19
14
13
12
7
6
6
6
6
6
6
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
518
275
243
145
138
137
113
67
49
42
32
11
10
10
8
8
5
1
1
1
1
1
Supplier
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Catalog No. ABIN126981
$ 412.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00087902317 ABIN184365 IHC (fro) IP WB Rabbit Log in to see Polyclonal

Similar anti-BCL2 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 27 Antibodies 55 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies 7 Antibodies
Flow Cytometry (FACS) 25 Antibodies 32 Antibodies
Functional Studies (Func) 1 Antibodies 1 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 5 Antibodies
Immunofluorescence (IF) 21 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 11 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 45 Antibodies
Immunohistochemistry (IHC) 32 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 18 Antibodies
Immunoprecipitation (IP) 15 Antibodies
Intracellular Staining (ICS) 5 Antibodies
Western Blotting (WB) 82 Antibodies
Fluorescence Microscopy (FM) 1 Antibodies

General

Antigen B-Cell CLL/lymphoma 2 (BCL2) Antibodies
Epitope N-Term
(76), (71), (53), (50), (47), (44), (23), (23), (19), (17), (15), (14), (14), (13), (13), (13), (13), (12), (12), (9), (7), (4), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(711), (199), (150), (17), (9), (7), (4), (3), (2), (2), (1), (1)
Host Rabbit
(493), (278), (19), (5), (2), (2), (2)
Clonality
Polyclonal
Conjugate This BCL2 antibody is un-conjugated
(40), (35), (30), (26), (19), (14), (13), (12), (7), (6), (6), (6), (6), (6), (6), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
(518), (275), (243), (145), (138), (137), (113), (67), (49), (42), (32), (11), (10), (10), (8), (8), (5), (1), (1), (1), (1), (1)
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Product Details anti-BCL2 Antibody

Target Details BCL2 Application Details Handling Images
Specificity Detects a 25 kDa protein, corresponding to the apparent molecular weight of the alpha isoform of Bcl-2 on SDS-PAGE immunoblots.
Purification Affinity-purified
Immunogen Synthetic peptide corresponding to the N-terminus of human Bcl-2

Target Details BCL2

Product Details anti-BCL2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Bcl-2 (BCL2 Antibody Abstract)
Background Bcl-2 and its homologs belong to a family of proteins that either promotes or prevent apoptosis. Bcl-2 alpha and beta are the alternatively spliced isoforms of 25 kDa and 22 kDa mitochondrial integral membrane proteins that inhibit apoptosis. Bcl-2 was identified as a gene translocated from chromosome 18 to the immunoglobulin (Ig) heavy chain locus on chromosome 14 in B lymphocytes, a condition that is associated with the onset of human follicular B cell lymphomas. This interchromosomal translocation leads to constitutive expression of Bcl-2, which results in increased survival and expansion of follicular center cell population.Synonyms: Apoptosis regulator Bcl-2, B-Cell CLL/lymphoma 2, BCL2, Bcl-2 alpha
Gene ID 596
NCBI Accession NP_000624
UniProt P10415
Research Area Cancer, Apoptosis/Necrosis
Pathways MAPK Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-BCL2 Antibody Target Details BCL2 Handling Images back to top
Application Notes Western blot analysis (0.5-4 μg/mL). Immunoprecipation (4-8 μg/mL). Immunohistochemistry on frozen sections (10-20 μg/mL).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details Images back to top
Concentration 0.2 mg/mL
Buffer PBS, pH 7.2, containing 30 % glycerol, 0.5 % BSA, 0.01 % thimerosal
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid Repeated freeze/defrost cycles.
Storage -20 °C
Storage Comment Store at -20 °C. For long-term storage, aliquot and freeze at -20 °C to -70 °C.

Images

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details Handling back to top
Supplier Images
 image for anti-BCL2 antibody (B-Cell CLL/lymphoma 2) (N-Term) (ABIN126981) anti-B-Cell CLL/lymphoma 2 (BCL2) (N-Term) antibody