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anti-Rat (Rattus) BCL2 antibody for Western Blotting

Recommended BCL2 Antibody (supplied by: Log in to see )

Antigen
B-Cell CLL/lymphoma 2 (BCL2) Antibodies
  • AW986256
  • bcl-2
  • BCL-2
  • Bcl-2
  • Bcl2
  • C430015F12Rik
  • D630044D05Rik
  • D830018M01Rik
  • PPP1R50
Epitope
N-Term
169
71
60
54
50
44
23
23
23
19
17
15
14
14
13
13
13
13
12
12
7
4
4
4
3
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
953
219
165
40
23
16
7
4
3
2
2
2
1
1
Host
Rabbit
523
504
19
6
2
2
2
Clonality
Polyclonal
Conjugate
This BCL2 antibody is un-conjugated
47
45
40
36
24
23
19
14
13
13
13
13
13
13
13
13
12
12
12
12
7
6
6
6
6
6
6
3
1
1
1
1
Application
Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
764
341
322
301
292
280
256
180
153
67
42
11
10
10
8
8
5
1
1
1
1
1
Supplier
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Catalog No. ABIN222888
$ 304.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.12814079 ABIN1105506 FACS WB Mouse IgG1 Log in to see 83-8B 2
0.12814079 ABIN256543 ICC IF IP WB Mouse IgG1 kappa N-Term, AA 61-76 Log in to see BCL-10C4 2
0.12814079 ABIN1531302 IHC ELISA WB Rabbit IgG pSer87 Log in to see Polyclonal 1
0.12814079 ABIN492372 WB Mouse IgG1 AA 61-76 Log in to see 10C4 1
0.12814079 ABIN968024 IF IP IHC WB Mouse IgG1 AA 49-179 Log in to see 7-Bcl 5
0.12814079 ABIN968025 IF IP IHC WB Mouse IgG1 AA 49-179 Log in to see 7-Bcl 5
0.12814079 ABIN683428 IF (cc) IF (p) IHC (p) WB Rabbit IgG AA 43-93, pSer70 Log in to see Polyclonal 1
0.12814079 ABIN950625 EIA IF IHC (p) WB Rabbit IgG AA 145-157, Internal Region Log in to see Polyclonal
0.12814079 ABIN2857047 IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal
0.12814079 ABIN1532505 IF IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.12814079 ABIN2705575 IHC WB Rabbit Center Log in to see Polyclonal
0.12814079 ABIN2705574 IHC WB Rabbit pSer69 Log in to see Polyclonal
0.12814079 ABIN1871251 WB Rabbit IgG Log in to see Polyclonal
0.12814079 ABIN2162973 IF IHC ELISA WB Rabbit IgG Thr69 Log in to see Polyclonal
0.12814079 ABIN1876758 IHC WB Rabbit IgG Log in to see Polyclonal
0.12814079 ABIN3183498 ELISA IF IHC WB Rabbit IgG Ser168, Ser170 Log in to see Polyclonal
0.12814079 ABIN3182532 ELISA IHC WB Rabbit IgG pSer87 Log in to see Polyclonal
0.12814079 ABIN4283369 IHC IHC (fro) IHC (p) IP WB Rabbit Log in to see Polyclonal 2
0.12814079 ABIN2425607 ELISA WB Rabbit IgG Log in to see Polyclonal
0.12814079 ABIN2431099 IHC ELISA WB Rabbit IgG Log in to see Polyclonal

Top referenced anti-BCL2 antibody for Western Blotting

Similar anti-BCL2 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 27 Antibodies 56 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies 7 Antibodies
Flow Cytometry (FACS) 25 Antibodies 33 Antibodies
Functional Studies (Func) 1 Antibodies 1 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 18 Antibodies
Immunofluorescence (IF) 34 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 11 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 45 Antibodies
Immunohistochemistry (IHC) 34 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 20 Antibodies
Immunoprecipitation (IP) 29 Antibodies
Intracellular Staining (ICS) 5 Antibodies
Western Blotting (WB) 96 Antibodies
Fluorescence Microscopy (FM) 1 Antibodies

General

Antigen B-Cell CLL/lymphoma 2 (BCL2) Antibodies
Epitope N-Term
(169), (71), (60), (54), (50), (44), (23), (23), (23), (19), (17), (15), (14), (14), (13), (13), (13), (13), (12), (12), (7), (4), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(953), (219), (165), (40), (23), (16), (7), (4), (3), (2), (2), (2), (1), (1)
Host Rabbit
(523), (504), (19), (6), (2), (2), (2)
Clonality
Polyclonal
Conjugate This BCL2 antibody is un-conjugated
(47), (45), (40), (36), (24), (23), (19), (14), (13), (13), (13), (13), (13), (13), (13), (13), (12), (12), (12), (12), (7), (6), (6), (6), (6), (6), (6), (3), (1), (1), (1), (1)
Application Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(764), (341), (322), (301), (292), (280), (256), (180), (153), (67), (42), (11), (10), (10), (8), (8), (5), (1), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-BCL2 Antibody

Target Details BCL2 Application Details Handling References for anti-BCL2 Antibody (ABIN222888) Images
Blocking Peptide Blocking peptide for this product available: ABIN412606
Specificity Detects a 25 kDa protein, corresponding to the apparent molecular weight of the ? isoform of Bcl-2 on SDS-PAGE immunoblots.
Purification Affinity purified
Immunogen Synthetic peptide corresponding to the N-terminus of human Bcl-2
Isotype IgG

Target Details BCL2

Product Details anti-BCL2 Antibody Application Details Handling References for anti-BCL2 Antibody (ABIN222888) Images back to top
Antigen
Alternative Name Bcl-2 (BCL2 Antibody Abstract)
Background Bcl-2 and its homologs belong to a family of proteins that either promotes or prevent apoptosis. Bcl-2 alpha and beta are the alternatively spliced isoforms of 25 kDa and 22 kDa mitochondrial integral membrane proteins that inhibit apoptosis.
Synonyms: BCL2, BCL 2
Gene ID 596
Research Area Cancer, Apoptosis/Necrosis
Pathways MAPK Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-BCL2 Antibody Target Details BCL2 Handling References for anti-BCL2 Antibody (ABIN222888) Images back to top
Application Notes Western blot analysis (0.5-4 μg/mL), Immunoprecipation (4-8 μg/mL), and Immunohistochemistry (10-20 μg/mL).
Comment

Country of Animal Origin: China

Restrictions For Research Use only

Handling

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details References for anti-BCL2 Antibody (ABIN222888) Images back to top
Format Liquid
Concentration 0.2 mg/mL
Buffer 100 μg (0.2 mg/mL) affinity purified rabbit anti-Bcl-2 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30 % glycerol, 0.5 % BSA, 0.01 % thimerosal.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The antibody solution should be gently mixed before use.
Avoid freeze-thaw cycles.
Storage -20 °C
Storage Comment For long-term storage, aliquot and freeze at -70 °C.
Expiry Date 12 months

References for anti-BCL2 Antibody (ABIN222888)

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details Handling Images back to top
Product cited in:

Eslami, Sharifi, Rahimi, Rahati: "Protective effect of telmisartan against oxidative damage induced by high glucose in neuronal PC12 cell." in: Neuroscience letters, 2013

Guérin, Cornut-Thibaut, Giscard-Destaing, Pouvreau, Guillemin, Aouacheria: "Subcellular dynamics of the maternal cell death regulator BCL2L10 in human preimplantation embryos." in: Human reproduction (Oxford, England), Vol. 28, Issue 3, pp. 729-39, 2013

Kiran Kumar, Prabhakara Rao, Noble, Weddington, McDowell, Rajanna, Bettaiya: "Lead-induced alteration of apoptotic proteins in different regions of adult rat brain." in: Toxicology letters, Vol. 184, Issue 1, pp. 56-60, 2008

Giuliani, Mioni, Altavilla, Leone, Bazzani, Minutoli, Bitto, Cainazzo, Marini, Zaffe, Botticelli, Pizzala, Savio, Necchi, Schiöth, Bertolini, Squadrito, Guarini: "Both early and delayed treatment with melanocortin 4 receptor-stimulating melanocortins produces neuroprotection in cerebral ischemia." in: Endocrinology, Vol. 147, Issue 3, pp. 1126-35, 2006

Images

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details Handling References for anti-BCL2 Antibody (ABIN222888) back to top
Supplier Images
Western Blotting (WB) image for anti-BCL2 antibody (B-Cell CLL/lymphoma 2) (N-Term) (ABIN222888) Western blot analysis of Bcl-2 expression in HL60 cell lysate.