anti-Human BRAF antibody for Immunocytochemistry

Recommended BRAF Antibody (supplied by: Log in to see )

Antigen
V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) Antibodies
  • B-Raf
  • braf1
  • rafb1
  • b-raf1
  • sierrabraf
  • BRAF
  • 9930012E13Rik
  • AA120551
  • AA387315
  • AA473386
  • B-raf
  • Braf-2
  • Braf2
  • C230098H17
  • C87398
  • D6Ertd631e
  • Braf
  • HYPT11
  • SME
  • Sm-E
  • B-RAF1
  • BRAF1
  • NS7
  • RAFB1
  • C-RMIL
  • RMIL
  • v-raf murine sarcoma viral oncogene homolog B
  • v-raf murine sarcoma viral oncogene homolog B1
  • Braf transforming gene
  • Braf transforming gene, related sequence 1
  • small nuclear ribonucleoprotein polypeptide E
  • braf
  • BRAF
  • LOC100219601
  • Braf
  • Braf-rs1
  • SNRPE
Reactivity
Human, Mouse (Murine)
406
84
69
13
10
4
4
3
1
1
1
1
Host
Mouse
309
93
3
2
Clonality
Monoclonal
Conjugate
This BRAF antibody is un-conjugated
20
20
20
17
17
17
3
3
3
3
3
3
3
3
3
3
3
Application
Immunocytochemistry (ICC), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
267
209
144
78
71
45
39
22
11
8
4
4
1
1
1
1
Options
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Catalog No. ABIN968991
$ 393.80
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2869395 ELISA ICC IHC WB Mouse IgG1 Log in to see 1H12 2
1 ABIN2830916 ICC IHC WB Rabbit IgG Val600Glu-Mutant Log in to see RM8
1 ABIN2617717 ICC IF IHC (p) WB Rabbit Internal Region Log in to see Polyclonal
1 ABIN5618894 ICC IF IHC WB Rabbit Center Log in to see Polyclonal

General

Antigen V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) Antibodies
Reactivity Human, Mouse (Murine)
(406), (84), (69), (13), (10), (4), (4), (3), (1), (1), (1), (1)
Host Mouse
(309), (93), (3), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This BRAF antibody is un-conjugated
(20), (20), (20), (17), (17), (17), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Application Immunocytochemistry (ICC), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(267), (209), (144), (78), (71), (45), (39), (22), (11), (8), (4), (4), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-BRAF Antibody

Target Details BRAF Application Details Handling ProductDetails: References for anti-BRAF antibody (ABIN968991) Images
Purification Ascites
Immunogen Purified recombinant fragment of human BRAF expressed in E. Coli.
Clone 1H12
Isotype IgG1

Target Details BRAF

Product Details anti-BRAF Antibody Application Details Handling ProductDetails: References for anti-BRAF antibody (ABIN968991) Images back to top
Antigen
Alternative Name BRAF (BRAF Antibody Abstract)
Background BRAF: v-raf murine sarcoma viral oncogene homolog B1, also known as BRAF1, RAFB1, B-RAF1, FLJ95109. Entrez Protein NP_004324. It is the main effectors recruited by GTP-bound Ras to activate the MEK-MAP kinase pathway. B-Raf contains three consensus Akt phosphorylationsites (Ser364, Ser428, and Thr439). B-Raf is a key regulatory molecule of the mitogen-activated protein kinase kinase (MEK), it has a long amino-terminal region,the region is essential for homo-dimerization of B-Raf and hetero-dimerization of B-Raf and c-Raf at the plasma membrane, followed by phosphorylation of Thr118 in the amino-terminal B-Raf-specific region. Notably, in calcium ionophore-stimulated HeLa cells, B-Raf could propagate signals to MEK under the basal level of GTP-Ras. Expression of Raf-B is highly restricted with highestlevels in the cerebrum and testes and defects in braf are involved in a wide range of cancers. The BRAF gene mutation is frequently detected in papillary thyroid carcinoma,melanocytic nevi, primary cutaneous melanomas and colorectal cancers.
Molecular Weight 87 kDa
Gene ID 673
HGNC 673
Pathways MAPK Signaling, RTK Signaling, Neurotrophin Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Hepatitis C, Autophagy

Application Details

Product Details anti-BRAF Antibody Target Details BRAF Handling ProductDetails: References for anti-BRAF antibody (ABIN968991) Images back to top
Application Notes ELISA: 1:10000, WB: 1:500 - 1:2000, IHC: 1:200 - 1:1000, ICC: 1:200 - 1:1000
Restrictions For Research Use only

Handling

Product Details anti-BRAF Antibody Target Details BRAF Application Details ProductDetails: References for anti-BRAF antibody (ABIN968991) Images back to top
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment 4°C, -20°C for long term storage

ProductDetails: References for anti-BRAF antibody (ABIN968991)

Product Details anti-BRAF Antibody Target Details BRAF Application Details Handling Images back to top
Product cited in:

Di Nicolantonio, Martini, Molinari, Sartore-Bianchi, Arena, Saletti, De Dosso, Mazzucchelli, Frattini, Siena, Bardelli: "Wild-type BRAF is required for response to panitumumab or cetuximab in metastatic colorectal cancer." in: Journal of clinical oncology : official journal of the American Society of Clinical Oncology, Vol. 26, Issue 35, pp. 5705-12, 2008

Riesco-Eizaguirre, Gutiérrez-Martínez, García-Cabezas, Nistal, Santisteban: "The oncogene BRAF V600E is associated with a high risk of recurrence and less differentiated papillary thyroid carcinoma due to the impairment of Na+/I- targeting to the membrane." in: Endocrine-related cancer, Vol. 13, Issue 1, pp. 257-69, 2006

Images

Product Details anti-BRAF Antibody Target Details BRAF Application Details Handling ProductDetails: References for anti-BRAF antibody (ABIN968991) back to top
Supplier Images
Immunocytochemistry (ICC) image for anti-V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) antibody (ABIN968991) Figure 1: Confocal immunofluorescence analysis of MCF-7 (left) and HepG2 (right) cell...
Immunohistochemistry (IHC) image for anti-V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) antibody (ABIN968991) Figure 2: Immunohistochemical analysis of paraffin-embedded human testis tissues usin...
Western Blotting (WB) image for anti-V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) antibody (ABIN968991) Figure 3: Western blot analysis using BRAF mouse mAb against Hela (1), HL60 (2), HepG...