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anti-Human BRAF antibody for Immunohistochemistry

Recommended BRAF Antibody (supplied by: Log in to see )

Antigen
V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) Antibodies
  • 9930012E13Rik
  • AA120551
  • AA387315
  • AA473386
  • B-raf
  • B-Raf
  • B-RAF1
  • b-raf1
  • Braf
  • BRAF
  • Braf-2
  • BRAF1
  • braf1
  • Braf2
  • C-RMIL
  • C87398
  • C230098H17
  • D6Ertd631e
  • HYPT11
  • NS7
  • rafb1
  • RAFB1
  • RMIL
  • sierrabraf
  • Sm-E
  • SME
Reactivity
Human, Mouse (Murine)
376
50
44
6
4
1
1
1
Host
Mouse
285
88
2
2
Clonality
Monoclonal
Conjugate
This BRAF antibody is un-conjugated
20
20
20
17
17
17
3
3
3
3
3
3
3
3
3
Application
Immunocytochemistry (ICC), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
248
211
149
83
43
41
33
16
4
3
2
1
1
1
Supplier
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Catalog No. ABIN968991
$ 393.80
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.2292046 ABIN2452438 IHC WB Mouse IgG2a Log in to see 4A5
0.2292046 ABIN2452440 FACS IF IHC WB Mouse IgG1 Log in to see 5A9
0.2292046 ABIN2452446 IHC WB Mouse IgG2a Log in to see 1G6
0.2292046 ABIN2452447 IF IHC WB Mouse IgG2b Log in to see 3E8
0.2292046 ABIN2452451 IHC WB Mouse IgG1 Log in to see 3B12
0.2292046 ABIN2452452 IHC WB Mouse IgG2b Log in to see 1G8
0.2292046 ABIN2452450 IHC WB Mouse IgG1 Log in to see 4G6
0.2292046 ABIN2452449 IHC WB Mouse IgG1 Log in to see 4C5
0.2292046 ABIN2452456 IHC WB Mouse IgG1 Log in to see 4C11
0.2292046 ABIN2452570 IF IHC WB Mouse IgG2a Log in to see 3A7
0.2292046 ABIN2452816 FACS IF IHC WB Mouse IgG1 Log in to see 1E2
0.2292046 ABIN2452814 FACS IF IHC WB Mouse IgG1 Log in to see 1D4
0.2292046 ABIN258430 FACS IHC (p) IP IHC WB Rabbit IgG Log in to see EP152Y
0.2292046 ABIN2469737 IF IHC Rabbit IgG Ser601, pThr598 Log in to see Polyclonal
0.2292046 ABIN2163049 IHC ELISA Rabbit IgG Thr598 Log in to see Polyclonal
0.2292046 ABIN2163053 IHC ELISA WB Rabbit IgG Ser601 Log in to see Polyclonal
0.2292046 ABIN2470813 IF IHC ELISA WB Mouse IgG1 Log in to see 1H12
0.2292046 ABIN2163046 IHC ELISA WB Rabbit IgG Ser446 Log in to see Polyclonal
0.2292046 ABIN2163047 IHC ELISA WB Rabbit IgG Ser446 Log in to see Polyclonal
0.2292046 ABIN1585648 IP IHC ELISA WB Rabbit N-Term Log in to see Polyclonal

Top referenced anti-BRAF antibody for Immunohistochemistry

Similar anti-BRAF Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 26 Antibodies 211 Antibodies
Flow Cytometry (FACS) 1 Antibodies 17 Antibodies
Immunocytochemistry (ICC) 2 Antibodies 4 Antibodies
Immunofluorescence (IF) 7 Antibodies 40 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies 33 Antibodies
Immunohistochemistry (IHC) 26 Antibodies 151 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 5 Antibodies
Immunoprecipitation (IP) 2 Antibodies
Western Blotting (WB) 38 Antibodies
Dot Blot (DB) 83 Antibodies
ELISA (Capture) 1 Antibodies
Functional Studies (Func) 1 Antibodies

General

Antigen V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) Antibodies
Reactivity Human, Mouse (Murine)
(376), (50), (44), (6), (4), (1), (1), (1)
Host Mouse
(285), (88), (2), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This BRAF antibody is un-conjugated
(20), (20), (20), (17), (17), (17), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Application Immunocytochemistry (ICC), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(248), (211), (149), (83), (43), (41), (33), (16), (4), (3), (2), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-BRAF Antibody

Target Details BRAF Application Details Handling References for anti-BRAF Antibody (ABIN968991) Images
Purification Ascites
Immunogen Purified recombinant fragment of human BRAF expressed in E. coli.
Clone 1H12
Isotype IgG1

Target Details BRAF

Product Details anti-BRAF Antibody Application Details Handling References for anti-BRAF Antibody (ABIN968991) Images back to top
Antigen
Alternative Name BRAF (BRAF Antibody Abstract)
Background BRAF: v-raf murine sarcoma viral oncogene homolog B1, also known as BRAF1, RAFB1, B-RAF1, FLJ95109. Entrez Protein NP_004324. It is the main effectors recruited by GTP-bound Ras to activate the MEK-MAP kinase pathway. B-Raf contains three consensus Akt phosphorylationsites (Ser364, Ser428, and Thr439). B-Raf is a key regulatory molecule of the mitogen-activated protein kinase kinase (MEK), it has a long amino-terminal region,the region is essential for homo-dimerization of B-Raf and hetero-dimerization of B-Raf and c-Raf at the plasma membrane, followed by phosphorylation of Thr118 in the amino-terminal B-Raf-specific region. Notably, in calcium ionophore-stimulated HeLa cells, B-Raf could propagate signals to MEK under the basal level of GTP-Ras. Expression of Raf-B is highly restricted with highestlevels in the cerebrum and testes and defects in braf are involved in a wide range of cancers. The BRAF gene mutation is frequently detected in papillary thyroid carcinoma,melanocytic nevi, primary cutaneous melanomas and colorectal cancers.
Molecular Weight 87 kDa
Gene ID 673
HGNC 673
Pathways MAPK Signaling, RTK Signaling, Neurotrophin Signaling Pathway

Application Details

Product Details anti-BRAF Antibody Target Details BRAF Handling References for anti-BRAF Antibody (ABIN968991) Images back to top
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, ICC: 1/200 - 1/1000
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only

Handling

Product Details anti-BRAF Antibody Target Details BRAF Application Details References for anti-BRAF Antibody (ABIN968991) Images back to top
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.

References for anti-BRAF Antibody (ABIN968991)

Product Details anti-BRAF Antibody Target Details BRAF Application Details Handling Images back to top
Product cited in:

Di Nicolantonio, Martini, Molinari, Sartore-Bianchi, Arena, Saletti, De Dosso, Mazzucchelli, Frattini, Siena, Bardelli: "Wild-type BRAF is required for response to panitumumab or cetuximab in metastatic colorectal cancer." in: Journal of clinical oncology : official journal of the American Society of Clinical Oncology, Vol. 26, Issue 35, pp. 5705-12, 2008

Riesco-Eizaguirre, Gutiérrez-Martínez, García-Cabezas, Nistal, Santisteban: "The oncogene BRAF V600E is associated with a high risk of recurrence and less differentiated papillary thyroid carcinoma due to the impairment of Na+/I- targeting to the membrane." in: Endocrine-related cancer, Vol. 13, Issue 1, pp. 257-69, 2006

Images

Product Details anti-BRAF Antibody Target Details BRAF Application Details Handling References for anti-BRAF Antibody (ABIN968991) back to top
Supplier Images
Immunocytochemistry (ICC) image for anti-BRAF antibody (V-Raf Murine Sarcoma Viral Oncogene Homolog B1) (ABIN968991) Figure 1: Confocal immunofluorescence analysis of MCF-7 (left) and HepG2 (right) cell...
Immunohistochemistry (IHC) image for anti-BRAF antibody (V-Raf Murine Sarcoma Viral Oncogene Homolog B1) (ABIN968991) Figure 2: Immunohistochemical analysis of paraffin-embedded human testis tissues usin...
Western Blotting (WB) image for anti-BRAF antibody (V-Raf Murine Sarcoma Viral Oncogene Homolog B1) (ABIN968991) Figure 3: Western blot analysis using BRAF mouse mAb against Hela (1), HL60 (2), HepG...