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anti-Human BRAF antibody for Western Blotting

Recommended BRAF Antibody (supplied by: Log in to see )

Antigen
V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) Antibodies
  • 9930012E13Rik
  • AA120551
  • AA387315
  • AA473386
  • B-raf
  • B-Raf
  • B-RAF1
  • b-raf1
  • Braf
  • BRAF
  • Braf-2
  • BRAF1
  • braf1
  • Braf2
  • C-RMIL
  • C87398
  • C230098H17
  • D6Ertd631e
  • HYPT11
  • NS7
  • rafb1
  • RAFB1
  • RMIL
  • sierrabraf
  • Sm-E
  • SME
Epitope
AA 379-408, Thr400
29
24
23
22
21
17
17
17
15
14
14
13
9
8
7
6
5
4
4
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
376
51
44
6
4
1
1
1
Host
Rabbit
284
89
2
2
Clonality
Polyclonal
Conjugate
This BRAF antibody is un-conjugated
20
20
20
17
17
17
3
3
3
3
3
3
3
3
3
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
248
212
150
83
42
41
33
16
4
4
2
1
1
1
Supplier
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Catalog No. ABIN392248
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.13398378 ABIN968991 ICC IHC ELISA WB Mouse IgG1 Log in to see 1H12 2
0.13398378 ABIN1724658 IHC ELISA WB Mouse IgG1 Log in to see 1H12F1 5
0.13398378 ABIN2169482 WB Mouse IgG2a Log in to see 4E1-E1-F9
0.13398378 ABIN1881118 FACS WB Rabbit Log in to see Polyclonal 6
0.13398378 ABIN513800 PLA ELISA WB Mouse IgG1 kappa AA 346-445, partial Log in to see 3D2 1
0.13398378 ABIN3043761 WB Rabbit IgG AA 38-230 Log in to see Polyclonal
0.13398378 ABIN3043016 WB Rabbit IgG AA 344-362, Middle Region Log in to see Polyclonal
0.13398378 ABIN2452280 WB Mouse IgG2a Log in to see 4B2
0.13398378 ABIN2452282 WB Mouse IgG1 Log in to see 3C3
0.13398378 ABIN2452447 IF IHC WB Mouse IgG2b Log in to see 3E8
0.13398378 ABIN2452815 FACS IF WB Mouse IgG1 Log in to see 1D2
0.13398378 ABIN258430 FACS IHC (p) IP IHC WB Rabbit IgG Log in to see EP152Y
0.13398378 ABIN1871340 IHC WB Rabbit IgG Log in to see Polyclonal
0.13398378 ABIN2163053 IHC ELISA WB Rabbit IgG Ser601 Log in to see Polyclonal
0.13398378 ABIN2163046 IHC ELISA WB Rabbit IgG Ser446 Log in to see Polyclonal
0.13398378 ABIN2163047 IHC ELISA WB Rabbit IgG Ser446 Log in to see Polyclonal
0.13398378 ABIN2163051 ELISA WB Rabbit IgG Thr753 Log in to see Polyclonal
0.13398378 ABIN2163052 ELISA WB Rabbit IgG Thr735 Log in to see Polyclonal
0.13398378 ABIN1585648 IP IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
0.13398378 ABIN931447 IHC ELISA WB Mouse IgG1 Log in to see 1H12F1-1H12G10-1F12F11C9

Top referenced anti-BRAF antibody for Western Blotting

Similar anti-BRAF Antibodies

Application / Reactivity Human
Dot Blot (DB) 83 Antibodies
ELISA 211 Antibodies
ELISA (Capture) 1 Antibodies
Flow Cytometry (FACS) 17 Antibodies
Functional Studies (Func) 1 Antibodies
Immunocytochemistry (ICC) 4 Antibodies
Immunofluorescence (IF) 40 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 33 Antibodies
Immunohistochemistry (IHC) 151 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 44 Antibodies
Immunoprecipitation (IP) 3 Antibodies
Luminex Assay (LMNX) 4 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Western Blotting (WB) 249 Antibodies

General

Antigen V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) Antibodies
Epitope AA 379-408, Thr400
(29), (24), (23), (22), (21), (17), (17), (17), (15), (14), (14), (13), (9), (8), (7), (6), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(376), (51), (44), (6), (4), (1), (1), (1)
Host Rabbit
(284), (89), (2), (2)
Clonality (Clone)
Polyclonal   ( )
Conjugate This BRAF antibody is un-conjugated
(20), (20), (20), (17), (17), (17), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(248), (212), (150), (83), (42), (41), (33), (16), (4), (4), (2), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-BRAF Antibody

Target Details BRAF Application Details Handling References for anti-BRAF Antibody (ABIN392248) Images
Specificity This BRAF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding T400 of human BRAF.
Predicted Reactivity Mouse (Murine)
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen This BRAF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 379-408 AA from human BRAF.
Clone RB13721
Isotype Ig

Target Details BRAF

Product Details anti-BRAF Antibody Application Details Handling References for anti-BRAF Antibody (ABIN392248) Images back to top
Antigen
Alternative Name BRAF (BRAF Antibody Abstract)
Background BRAF, a member of the RAF subfamily of Ser/Thr protein kinases, is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It may play a role in the postsynaptic responses of hippocampal neurons. This cytoplasmic protein is expressed in brain and testis. Defects in BRAF are involved in a wide range of cancers including lung cancer and non-Hodgkin lymphoma (NHL). This protein contains 1 zinc-dependent phorbol-ester and DAG binding domain.
Synonyms: B-Raf proto-oncogene serine/threonine-protein kinase, p94, v-Raf murine sarcoma viral oncogene homolog B1
Molecular Weight 84490 DA
Gene ID 673
UniProt P15056
Research Area Apoptosis/Necrosis, Protein Modifications, Cell Structure, Cancer, Neurology
Pathways MAPK Signaling, RTK Signaling, Neurotrophin Signaling Pathway

Application Details

Product Details anti-BRAF Antibody Target Details BRAF Handling References for anti-BRAF Antibody (ABIN392248) Images back to top
Application Notes WB = 1:1000, IHC (p) = 1:10-50
Restrictions For Research Use only

Handling

Product Details anti-BRAF Antibody Target Details BRAF Application Details References for anti-BRAF Antibody (ABIN392248) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-BRAF Antibody (ABIN392248)

Product Details anti-BRAF Antibody Target Details BRAF Application Details Handling Images back to top
Background publications

Hingorani, Jacobetz, Robertson, Herlyn, Tuveson: "Suppression of BRAF(V599E) in human melanoma abrogates transformation." in: Cancer research, Vol. 63, Issue 17, pp. 5198-202, 2003

Davies, Bignell, Cox, Stephens, Edkins, Clegg, Teague, Woffendin, Garnett, Bottomley, Davis, Dicks, Ewing, Floyd, Gray, Hall, Hawes, Hughes, Kosmidou, Menzies, Mould, Parker, Stevens, Watt, Hooper et al.: "Mutations of the BRAF gene in human cancer. ..." in: Nature, Vol. 417, Issue 6892, pp. 949-54, 2002

Naoki, Chen, Richards, Sugarbaker, Meyerson: "Missense mutations of the BRAF gene in human lung adenocarcinoma." in: Cancer research, Vol. 62, Issue 23, pp. 7001-3, 2002

Images

Product Details anti-BRAF Antibody Target Details BRAF Application Details Handling References for anti-BRAF Antibody (ABIN392248) back to top
Supplier Images
Western Blotting (WB) image for anti-BRAF antibody (V-Raf Murine Sarcoma Viral Oncogene Homolog B1) (AA 379-408) (ABIN392248) Western blot analysis of BRAF (arrow) using rabbit polyclonal BRAF Antibody (T400) (A...
Immunohistochemistry (IHC) image for anti-BRAF antibody (V-Raf Murine Sarcoma Viral Oncogene Homolog B1) (AA 379-408) (ABIN392248) Formalin-fixed and paraffin-embedded human brain tissue reacted with BRAF-pT400, whic...