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anti-Mouse (Murine) BRAF antibody for Immunofluorescence

Recommended BRAF Antibody (supplied by: Log in to see )

Antigen
V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) Antibodies
  • 9930012E13Rik
  • AA120551
  • AA387315
  • AA473386
  • B-raf
  • B-Raf
  • B-RAF1
  • b-raf1
  • Braf
  • BRAF
  • Braf-2
  • BRAF1
  • braf1
  • Braf2
  • C-RMIL
  • C87398
  • C230098H17
  • D6Ertd631e
  • HYPT11
  • NS7
  • rafb1
  • RAFB1
  • RMIL
  • sierrabraf
  • Sm-E
  • SME
Reactivity
Human, Mouse (Murine)
394
58
47
6
4
1
1
1
Host
Mouse
299
92
3
2
Clonality
Monoclonal
Conjugate
This BRAF antibody is un-conjugated
20
20
20
17
17
17
6
3
3
3
3
3
3
3
3
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
257
214
148
82
52
43
36
16
10
5
4
2
1
1
1
Supplier
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Catalog No. ABIN1105489
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.26663563 ABIN2163048 IF IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.0011003735 ABIN2470813 IF IHC ELISA WB Mouse IgG1 Log in to see 1H12
0.0011003735 ABIN2962049 IF IHC (p) ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal
0.0011003735 ABIN1583592 IF IHC WB Mouse IgG1 Log in to see 1H12
0.0011003735 ABIN4889012 ELISA IF WB Rabbit Internal Region Log in to see Polyclonal
0.0011003735 ABIN4889013 ELISA IF WB Rabbit Val600Glu-Mutant Log in to see Polyclonal
0.0011003735 ABIN1842978 ICC IF IHC ELISA WB Mouse IgG1 Log in to see
0.0011003735 ABIN1575079 IF IHC ELISA WB Mouse Log in to see

Top referenced anti-BRAF antibody for Immunofluorescence

Similar anti-BRAF Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 28 Antibodies 212 Antibodies
Immunocytochemistry (ICC) 3 Antibodies 5 Antibodies
Immunofluorescence (IF) 9 Antibodies 42 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 12 Antibodies 36 Antibodies
Immunohistochemistry (IHC) 24 Antibodies 148 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 8 Antibodies
Immunoprecipitation (IP) 1 Antibodies
Western Blotting (WB) 42 Antibodies
Dot Blot (DB) 82 Antibodies
ELISA (Capture) 1 Antibodies
Flow Cytometry (FACS) 16 Antibodies
Functional Studies (Func) 1 Antibodies

General

Antigen V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) Antibodies
Reactivity Human, Mouse (Murine)
(394), (58), (47), (6), (4), (1), (1), (1)
Host Mouse
(299), (92), (3), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This BRAF antibody is un-conjugated
(20), (20), (20), (17), (17), (17), (6), (3), (3), (3), (3), (3), (3), (3), (3)
Application Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(257), (214), (148), (82), (52), (43), (36), (16), (10), (5), (4), (2), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-BRAF Antibody

Target Details BRAF Application Details Handling References for anti-BRAF Antibody (ABIN1105489) Images
Specificity Recognizes Threonine Protein Kinase BRAF
Cross-Reactivity (Details) Species reactivity (tested):Human, Mouse.
Purification Ascites
Clone 1H12
Isotype IgG1

Target Details BRAF

Product Details anti-BRAF Antibody Application Details Handling References for anti-BRAF Antibody (ABIN1105489) Images back to top
Antigen
Background BRAF: v-raf murine sarcoma viral oncogene homolog B1, also known as BRAF1, RAFB1, B-RAF1, FLJ95109 is the main effectors recruited by GTP-bound Ras to activate the MEK-MAP kinase pathway. B-Raf contains three consensus Akt phosphorylationsites (Ser364, Ser428, and Thr439). B-Raf is a key regulatory molecule of the mitogen-activated protein kinase kinase (MEK), it has a long amino-terminal region,the region is essential for homo-dimerization of B-Raf and hetero-dimerization of B-Raf and c-Raf at the plasma membrane, followed by phosphorylation of Thr118 in the amino-terminal B-Raf-specific region. Notably, in calcium ionophore-stimulated HeLa cells, B-Raf could propagate signals to MEK under the basal level of GTP-Ras. Expression of Raf-B is highly restricted with highestlevels in the cerebrum and testes and defects in braf are involved in a wide range of cancers. The BRAF gene mutation is frequently detected in papillary thyroid carcinoma,melanocytic nevi, primary cutaneous melanomas and colorectal cancers.Synonyms: B-Raf proto-oncogene serine/threonine-protein kinase, BRAF, BRAF1, RAFB1, p94, v-Raf murine sarcoma viral oncogene homolog B1
Molecular Weight 87 kDa
Gene ID 673
Pathways MAPK Signaling, RTK Signaling, Neurotrophin Signaling Pathway

Application Details

Product Details anti-BRAF Antibody Target Details BRAF Handling References for anti-BRAF Antibody (ABIN1105489) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-BRAF Antibody Target Details BRAF Application Details References for anti-BRAF Antibody (ABIN1105489) Images back to top
Format Liquid

References for anti-BRAF Antibody (ABIN1105489)

Product Details anti-BRAF Antibody Target Details BRAF Application Details Handling Images back to top
Background publications

Riesco-Eizaguirre, Gutiérrez-Martínez, García-Cabezas, Nistal, Santisteban: "The oncogene BRAF V600E is associated with a high risk of recurrence and less differentiated papillary thyroid carcinoma due to the impairment of Na+/I- targeting to the membrane." in: Endocrine-related cancer, Vol. 13, Issue 1, pp. 257-69, 2006

Sherrill, Kyte: "Activation of epidermal growth factor receptor by epidermal growth factor." in: Biochemistry, Vol. 35, Issue 18, pp. 5705-18, 1996

Images

Product Details anti-BRAF Antibody Target Details BRAF Application Details Handling References for anti-BRAF Antibody (ABIN1105489) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-BRAF antibody (V-Raf Murine Sarcoma Viral Oncogene Homolog B1) (ABIN1105489) anti-V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) antibody
Immunofluorescence (IF) image for anti-BRAF antibody (V-Raf Murine Sarcoma Viral Oncogene Homolog B1) (ABIN1105489) anti-V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) antibody (Image 2)
Western Blotting (WB) image for anti-BRAF antibody (V-Raf Murine Sarcoma Viral Oncogene Homolog B1) (ABIN1105489) anti-V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) antibody (Image 3)