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anti-Mouse (Murine) BRAF antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended BRAF Antibody (supplied by: Log in to see )

Antigen
V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) Antibodies
  • B-Raf
  • braf1
  • rafb1
  • b-raf1
  • sierrabraf
  • BRAF
  • 9930012E13Rik
  • AA120551
  • AA387315
  • AA473386
  • B-raf
  • Braf-2
  • Braf2
  • C230098H17
  • C87398
  • D6Ertd631e
  • Braf
  • HYPT11
  • SME
  • Sm-E
  • B-RAF1
  • BRAF1
  • NS7
  • RAFB1
  • C-RMIL
  • RMIL
  • v-raf murine sarcoma viral oncogene homolog B
  • v-raf murine sarcoma viral oncogene homolog B1
  • Braf transforming gene
  • Braf transforming gene, related sequence 1
  • small nuclear ribonucleoprotein polypeptide E
  • braf
  • BRAF
  • LOC100219601
  • Braf
  • Braf-rs1
  • SNRPE
Reactivity
Human, Mouse (Murine)
405
59
48
6
4
1
1
1
Host
Rabbit
310
92
3
2
Clonality
Polyclonal
Conjugate
This BRAF antibody is un-conjugated
20
20
20
17
17
17
3
3
3
3
3
3
3
3
3
3
3
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
256
213
147
82
52
44
39
16
11
5
4
2
1
1
1
Supplier
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Catalog No. ABIN499008
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1105489 EIA IF IHC (p) WB Mouse IgG1 Log in to see 1H12 2
1 ABIN272000 IHC (p) Rabbit Log in to see Polyclonal
1 ABIN271999 IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN1711466 IHC (p) WB HRP Rabbit IgG pThr753 Log in to see Polyclonal
1 ABIN1700449 IHC (p) WB Biotin Rabbit IgG pThr753 Log in to see Polyclonal
1 ABIN2962049 IF IHC (p) ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal
1 ABIN1713951 IF (p) IHC (p) WB Rabbit IgG pThr753 Log in to see Polyclonal

Top referenced anti-BRAF antibody for Immunohistochemistry (Paraffin-embedded Sections)

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Immunocytochemistry (ICC) 3 Antibodies 5 Antibodies
Immunofluorescence (IF) 9 Antibodies 42 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
Immunohistochemistry (IHC) 24 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 8 Antibodies
Immunoprecipitation (IP) 1 Antibodies
Western Blotting (WB) 42 Antibodies
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ELISA (Capture) 1 Antibodies
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General

Antigen V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) Antibodies
Reactivity Human, Mouse (Murine)
(405), (59), (48), (6), (4), (1), (1), (1)
Host Rabbit
(310), (92), (3), (2)
Clonality
Polyclonal
Conjugate This BRAF antibody is un-conjugated
(20), (20), (20), (17), (17), (17), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(256), (213), (147), (82), (52), (44), (39), (16), (11), (5), (4), (2), (1), (1), (1)
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Product Details anti-BRAF Antibody

Target Details BRAF Application Details Handling Images
Specificity This antibody detects endogenous levels of Raf-B protein. (region surrounding Lys439)
Cross-Reactivity (Details) Species reactivity (tested):Human, Mouse.
Purification Affinity chromatography
Purity > 95 % by SDS-PAGE

Target Details BRAF

Product Details anti-BRAF Antibody Application Details Handling Images back to top
Antigen
Background Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. Raf-A, a second member of the Raf gene family of serine/threonine protein kinases, exhibits substantial homology to Raf-1 within the kinase domain of the two molecules, but less homology elsewhere. Expression of Raf-B is highly restricted with highest levels in the cerebrum and testis.Synonyms: B-Raf proto-oncogene serine/threonine-protein kinase, BRAF, BRAF1, RAFB1, p94, v-Raf murine sarcoma viral oncogene homolog B1
Molecular Weight approx. 86 kDa
Gene ID 673
NCBI Accession NP_004324
UniProt P15056
Pathways MAPK Signaling, RTK Signaling, Neurotrophin Signaling Pathway

Application Details

Product Details anti-BRAF Antibody Target Details BRAF Handling Images back to top
Application Notes Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-BRAF Antibody Target Details BRAF Application Details Images back to top
Concentration 1,0 mg/mL
Buffer Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Images

Product Details anti-BRAF Antibody Target Details BRAF Application Details Handling back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-BRAF antibody (V-Raf Murine Sarcoma Viral Oncogene Homolog B1) (ABIN499008) Immunohistochemistry (IHC) analyzes of Raf-B antibody in paraffin-embedded hum...
Western Blotting (WB) image for anti-BRAF antibody (V-Raf Murine Sarcoma Viral Oncogene Homolog B1) (ABIN499008) Western blot (WB) analysis of Raf-B antibody in extracts from 293 cells.