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anti-Mouse (Murine) BRAF antibody for Western Blotting

Recommended BRAF Antibody (supplied by: Log in to see )

Antigen
V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) Antibodies
  • B-Raf
  • braf1
  • rafb1
  • b-raf1
  • sierrabraf
  • BRAF
  • 9930012E13Rik
  • AA120551
  • AA387315
  • AA473386
  • B-raf
  • Braf-2
  • Braf2
  • C230098H17
  • C87398
  • D6Ertd631e
  • Braf
  • HYPT11
  • SME
  • Sm-E
  • B-RAF1
  • BRAF1
  • NS7
  • RAFB1
  • C-RMIL
  • RMIL
  • v-raf murine sarcoma viral oncogene homolog B
  • v-raf murine sarcoma viral oncogene homolog B1
  • Braf transforming gene
  • Braf transforming gene, related sequence 1
  • small nuclear ribonucleoprotein polypeptide E
  • braf
  • BRAF
  • LOC100219601
  • Braf
  • Braf-rs1
  • SNRPE
Reactivity
Human, Mouse (Murine)
400
57
46
6
4
1
1
1
Host
Mouse
305
92
3
2
Clonality
Monoclonal
Conjugate
This BRAF antibody is un-conjugated
20
20
20
17
17
17
3
3
3
3
3
3
3
3
3
Application
Immunocytochemistry (ICC), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
257
213
147
82
53
44
33
16
11
4
4
2
1
1
1
Options
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Catalog No. ABIN968991
$ 393.80
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
2.9590793 ABIN3043761 WB Rabbit IgG AA 38-230 Log in to see Polyclonal
2.9590793 ABIN783620 EIA WB Chicken Center Log in to see Polyclonal
2.9590793 ABIN1105489 EIA IF IHC (p) WB Mouse IgG1 Log in to see 1H12 2
2.9590793 ABIN499008 IHC (p) WB Rabbit Log in to see Polyclonal
2.9590793 ABIN1871340 IHC WB Rabbit IgG Log in to see Polyclonal
2.9590793 ABIN2163052 ELISA WB Rabbit IgG Thr735 Log in to see Polyclonal
2.9590793 ABIN2163047 IHC ELISA WB Rabbit IgG Ser446 Log in to see Polyclonal
2.9590793 ABIN2163046 IHC ELISA WB Rabbit IgG Ser446 Log in to see Polyclonal
2.9590793 ABIN2163051 ELISA WB Rabbit IgG Thr753 Log in to see Polyclonal
2.9590793 ABIN2163053 IHC ELISA WB Rabbit IgG Ser601 Log in to see Polyclonal
2.9590793 ABIN1585648 IP IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
2.9590793 ABIN2163048 IF IHC ELISA WB Rabbit IgG Log in to see Polyclonal
2.9590793 ABIN2431117 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
2.9590793 ABIN2425629 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
2.9590793 ABIN2426109 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
2.9590793 ABIN2736363 WB Rabbit IgG Log in to see Polyclonal
2.9590793 ABIN1870020 WB Rabbit IgG pSer445 Log in to see Polyclonal
2.9590793 ABIN2561461 WB Rabbit IgG pSer728 Log in to see Polyclonal
2.9590793 ABIN3029546 WB Rabbit IgG AA 38-230 Log in to see Polyclonal
2.9590793 ABIN1842978 ICC IF IHC ELISA WB Mouse IgG1 Log in to see

Top referenced anti-BRAF antibody for Western Blotting

  • Human Monoclonal BRAF Primary Antibody for ICC, IHC - ABIN968991 (2 Publications): Di Nicolantonio, Martini, Molinari, Sartore-Bianchi, Arena, Saletti, De Dosso, Mazzucchelli, Frattini, Siena, Bardelli: Wild-type BRAF is required for response to panitumumab or cetuximab in metastatic colorectal cancer. in Journal of clinical oncology : official journal of the American Society of Clinical Oncology 2008 (PubMed)

Similar anti-BRAF Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 28 Antibodies 212 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 11 Antibodies
Immunocytochemistry (ICC) 3 Antibodies 5 Antibodies
Immunofluorescence (IF) 9 Antibodies 42 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 24 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 8 Antibodies
Immunoprecipitation (IP) 1 Antibodies
Western Blotting (WB) 42 Antibodies
Dot Blot (DB) 82 Antibodies
ELISA (Capture) 1 Antibodies
Flow Cytometry (FACS) 16 Antibodies
Functional Studies (Func) 1 Antibodies

General

Antigen V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) Antibodies
Reactivity Human, Mouse (Murine)
(400), (57), (46), (6), (4), (1), (1), (1)
Host Mouse
(305), (92), (3), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This BRAF antibody is un-conjugated
(20), (20), (20), (17), (17), (17), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Application Immunocytochemistry (ICC), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(257), (213), (147), (82), (53), (44), (33), (16), (11), (4), (4), (2), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-BRAF Antibody

Target Details BRAF Application Details Handling References for anti-BRAF Antibody (ABIN968991) Images
Purification Ascites
Immunogen Purified recombinant fragment of human BRAF expressed in E. coli.
Clone 1H12
Isotype IgG1

Target Details BRAF

Product Details anti-BRAF Antibody Application Details Handling References for anti-BRAF Antibody (ABIN968991) Images back to top
Antigen
Alternative Name BRAF (BRAF Antibody Abstract)
Background BRAF: v-raf murine sarcoma viral oncogene homolog B1, also known as BRAF1, RAFB1, B-RAF1, FLJ95109. Entrez Protein NP_004324. It is the main effectors recruited by GTP-bound Ras to activate the MEK-MAP kinase pathway. B-Raf contains three consensus Akt phosphorylationsites (Ser364, Ser428, and Thr439). B-Raf is a key regulatory molecule of the mitogen-activated protein kinase kinase (MEK), it has a long amino-terminal region,the region is essential for homo-dimerization of B-Raf and hetero-dimerization of B-Raf and c-Raf at the plasma membrane, followed by phosphorylation of Thr118 in the amino-terminal B-Raf-specific region. Notably, in calcium ionophore-stimulated HeLa cells, B-Raf could propagate signals to MEK under the basal level of GTP-Ras. Expression of Raf-B is highly restricted with highestlevels in the cerebrum and testes and defects in braf are involved in a wide range of cancers. The BRAF gene mutation is frequently detected in papillary thyroid carcinoma,melanocytic nevi, primary cutaneous melanomas and colorectal cancers.
Molecular Weight 87 kDa
Gene ID 673
HGNC 673
Pathways MAPK Signaling, RTK Signaling, Neurotrophin Signaling Pathway

Application Details

Product Details anti-BRAF Antibody Target Details BRAF Handling References for anti-BRAF Antibody (ABIN968991) Images back to top
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, ICC: 1/200 - 1/1000
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only

Handling

Product Details anti-BRAF Antibody Target Details BRAF Application Details References for anti-BRAF Antibody (ABIN968991) Images back to top
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.

References for anti-BRAF Antibody (ABIN968991)

Product Details anti-BRAF Antibody Target Details BRAF Application Details Handling Images back to top
Product cited in:

Di Nicolantonio, Martini, Molinari, Sartore-Bianchi, Arena, Saletti, De Dosso, Mazzucchelli, Frattini, Siena, Bardelli: "Wild-type BRAF is required for response to panitumumab or cetuximab in metastatic colorectal cancer." in: Journal of clinical oncology : official journal of the American Society of Clinical Oncology, Vol. 26, Issue 35, pp. 5705-12, 2008

Riesco-Eizaguirre, Gutiérrez-Martínez, García-Cabezas, Nistal, Santisteban: "The oncogene BRAF V600E is associated with a high risk of recurrence and less differentiated papillary thyroid carcinoma due to the impairment of Na+/I- targeting to the membrane." in: Endocrine-related cancer, Vol. 13, Issue 1, pp. 257-69, 2006

Images

Product Details anti-BRAF Antibody Target Details BRAF Application Details Handling References for anti-BRAF Antibody (ABIN968991) back to top
Supplier Images
Immunocytochemistry (ICC) image for anti-BRAF antibody (V-Raf Murine Sarcoma Viral Oncogene Homolog B1) (ABIN968991) Figure 1: Confocal immunofluorescence analysis of MCF-7 (left) and HepG2 (right) cell...
Immunohistochemistry (IHC) image for anti-BRAF antibody (V-Raf Murine Sarcoma Viral Oncogene Homolog B1) (ABIN968991) Figure 2: Immunohistochemical analysis of paraffin-embedded human testis tissues usin...
Western Blotting (WB) image for anti-BRAF antibody (V-Raf Murine Sarcoma Viral Oncogene Homolog B1) (ABIN968991) Figure 3: Western blot analysis using BRAF mouse mAb against Hela (1), HL60 (2), HepG...