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anti-Mouse (Murine) BRAF antibody for Western Blotting

Recommended BRAF Antibody (supplied by: Log in to see )

Antigen
V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) Antibodies
  • B-Raf
  • braf1
  • rafb1
  • b-raf1
  • sierrabraf
  • BRAF
  • 9930012E13Rik
  • AA120551
  • AA387315
  • AA473386
  • B-raf
  • Braf-2
  • Braf2
  • C230098H17
  • C87398
  • D6Ertd631e
  • Braf
  • HYPT11
  • SME
  • Sm-E
  • B-RAF1
  • BRAF1
  • NS7
  • RAFB1
  • C-RMIL
  • RMIL
  • v-raf murine sarcoma viral oncogene homolog B
  • v-raf murine sarcoma viral oncogene homolog B1
  • Braf transforming gene
  • Braf transforming gene, related sequence 1
  • small nuclear ribonucleoprotein polypeptide E
  • braf
  • BRAF
  • LOC100219601
  • Braf
  • Braf-rs1
  • SNRPE
Epitope
AA 38-230
35
26
24
22
22
21
19
18
18
15
15
13
9
9
6
6
6
5
4
4
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
395
83
67
13
10
4
4
3
1
1
1
1
Host
Rabbit
299
92
3
2
Clonality
Polyclonal
Conjugate
This BRAF antibody is un-conjugated
20
20
20
17
17
17
3
3
3
3
3
3
3
3
3
3
3
Application
Western Blotting (WB)
258
203
141
78
64
42
39
22
11
8
4
4
1
1
1
1
Options
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Catalog No. ABIN3043761
$ 240.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.1870947 ABIN968991 ICC IHC ELISA WB Mouse IgG1 Log in to see 1H12 2
3.1870947 ABIN783620 EIA WB Chicken Center Log in to see Polyclonal
3.1870947 ABIN1105489 EIA IF IHC (p) WB Mouse IgG1 Log in to see 1H12 2
3.1870947 ABIN499008 IHC (p) WB Rabbit Log in to see Polyclonal
3.1870947 ABIN1871340 IHC WB Rabbit IgG Log in to see Polyclonal
3.1870947 ABIN1585648 IP IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
3.1870947 ABIN2736363 WB Rabbit IgG Log in to see Polyclonal
3.1870947 ABIN2561461 WB Rabbit IgG pSer728 Log in to see Polyclonal
3.1870947 ABIN1870020 WB Rabbit IgG pSer445 Log in to see Polyclonal
3.1870947 ABIN2426109 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
3.1870947 ABIN3029546 WB Rabbit IgG AA 38-230 Log in to see Polyclonal
3.1870947 ABIN3029541 WB Rabbit IgG AA 344-362 Log in to see Polyclonal
3.1870947 ABIN1842978 ELISA IF/ICC IHC WB Mouse IgG1 Log in to see
3.1870947 ABIN2425629 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
3.1870947 ABIN2431117 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN2869395 ELISA ICC IHC WB Mouse IgG1 Log in to see 1H12 2
1 ABIN462545 IF IHC (p) ELISA WB Mouse IgG1 AA 285-401 Log in to see 1H12
1 ABIN2888763 ELISA IHC WB Rabbit IgG AA 576-625 Log in to see Polyclonal
1 ABIN2879057 IF IHC (p) ELISA WB Rabbit IgG AA 171-220 Log in to see Polyclonal
1 ABIN2878975 IHC (p) ELISA WB Rabbit IgG AA 411-460 Log in to see Polyclonal

Top referenced anti-BRAF antibody for Western Blotting

  • Human Monoclonal BRAF Primary Antibody for ICC, IHC - ABIN968991 (2 Publications): Di Nicolantonio, Martini, Molinari, Sartore-Bianchi, Arena, Saletti, De Dosso, Mazzucchelli, Frattini, Siena, Bardelli: Wild-type BRAF is required for response to panitumumab or cetuximab in metastatic colorectal cancer. in Journal of clinical oncology : official journal of the American Society of Clinical Oncology 2008 (PubMed)

Similar anti-BRAF Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus)
Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies 1 Antibodies
Western Blotting (WB) 258 Antibodies 61 Antibodies 44 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Luminex Assay (LMNX) 4 Antibodies
Immunoprecipitation (IP) 8 Antibodies 2 Antibodies 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 64 Antibodies 26 Antibodies 19 Antibodies
Immunohistochemistry (IHC) 141 Antibodies 24 Antibodies 16 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 39 Antibodies 13 Antibodies

General

Antigen V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) Antibodies
Epitope AA 38-230
(35), (26), (24), (22), (22), (21), (19), (18), (18), (15), (15), (13), (9), (9), (6), (6), (6), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(395), (83), (67), (13), (10), (4), (4), (3), (1), (1), (1), (1)
Host Rabbit
(299), (92), (3), (2)
Clonality
Polyclonal
Conjugate This BRAF antibody is un-conjugated
(20), (20), (20), (17), (17), (17), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Application Western Blotting (WB)
(258), (203), (141), (78), (64), (42), (39), (22), (11), (8), (4), (4), (1), (1), (1), (1)
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Product Details anti-BRAF Antibody

Target Details BRAF Application Details Handling Images
Purpose Rabbit IgG polyclonal antibody for Serine/threonine-protein kinase B-raf(BRAF) detection. Tested with WB in Human,Mouse,Rat.
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Rabbit IgG polyclonal antibody for Serine/threonine-protein kinase B-raf(BRAF) detection. Tested with WB in Human,Mouse,Rat.
Gene Name: B-Raf proto-oncogene, serine/threonine kinase
Protein Name: Serine/threonine-protein kinase B-raf
Purification Immunogen affinity purified.
Immunogen E.coli-derived human B Raf recombinant protein (Position: A38-V230). Human B Raf shares 81% amino acid (aa) sequence identity with mouse B Raf.
Isotype IgG

Target Details BRAF

Product Details anti-BRAF Antibody Application Details Handling Images back to top
Antigen
Alternative Name BRAF (BRAF Antibody Abstract)
Background BRAF (v-raf murine sarcoma viral oncogene homolog B1) is a human gene that makes a protein called B-Raf. It is a member of the Raf kinase family of growth signal transduction protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. It is mapped to 7q34. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. The BRAF protein is also involved in sending signals inside cells, which are involved in directing cell growth.

Synonyms: FLJ95109 antibody|94 kDa B raf protein antibody|B raf 1 antibody|B raf 1 antibody|B Raf proto oncogene serine threonine protein kinase antibody|B Raf proto oncogene serine threonine protein kinase antibody|B-Raf proto-oncogene serine/threonine-protein kinase (p94) antibody|BRAF 1 antibody|BRaf antibody|BRaf antibody|BRAF_HUMAN antibody|BRAF1 antibody|BRAF1 antibody|cRmil antibody|MGC126806 antibody|MGC138284 antibody|Murine sarcoma viral (v-raf) oncogene homolog B1 antibody|Murine sarcoma viral (v-raf) oncogene homolog B1 antibody|Murine sarcoma viral v raf oncogene homolog B1 antibody|Murine sarcoma viral v raf oncogene homolog B1 antibody|NS7 antibody|oncogene BRAF1 antibody|p94 antibody|p94 antibody|Proto-oncogene B-Raf antibody|Proto-oncogene c-Rmil antibody|RAFB 1 antibody|RAFB1 antibody|RAFB1 antibody|RMIL antibody|Serine/threonine-protein kinase B-raf antibody|v raf murine sarcoma viral oncogene homolog B antibody|v raf murine sarcoma viral oncogene homolog B1 antibody|v raf murine sarcoma viral oncogene homolog B1 antibody|v-Raf murine sarcoma viral oncogene homolog B1 antibody
Gene ID 673
UniProt P15056
Pathways MAPK Signaling, RTK Signaling, Neurotrophin Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Hepatitis C, Autophagy

Application Details

Product Details anti-BRAF Antibody Target Details BRAF Handling Images back to top
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat, The detection limit for B Raf is approximately 0.25 ng/lane under reducing conditions.
Notes: Tested Species: Species with positive results.
Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions For Research Use only

Handling

Product Details anti-BRAF Antibody Target Details BRAF Application Details Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Images

Product Details anti-BRAF Antibody Target Details BRAF Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-BRAF antibody (V-Raf Murine Sarcoma Viral Oncogene Homolog B1) (AA 38-230) (ABIN3043761) anti-V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) (AA 38-230) antibody
Western Blotting (WB) image for anti-BRAF antibody (V-Raf Murine Sarcoma Viral Oncogene Homolog B1) (AA 38-230) (ABIN3043761) anti-V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) (AA 38-230) antibody (Image 2)