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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)|
|Western Blotting (WB)||256 Antibodies||42 Antibodies||29 Antibodies|
|Proximity Ligation Assay (PLA)||1 Antibodies|
|Luminex Assay (LMNX)||4 Antibodies|
|Immunoprecipitation (IP)||2 Antibodies||1 Antibodies||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||53 Antibodies||8 Antibodies||6 Antibodies|
|Immunohistochemistry (IHC)||148 Antibodies||24 Antibodies||15 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||36 Antibodies||12 Antibodies||12 Antibodies|
|Immunofluorescence (IF)||42 Antibodies||9 Antibodies|
|Antigen||V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This BRAF antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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Product Details anti-BRAF AntibodyTarget Details BRAF Application Details Handling Images
|Specificity||This antibody detects endogenous levels of B-Raf protein. (region surrounding Phe594)|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Purification||The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen|
|Purity||> 95 % pure by SDS-PAGE|
Target Details BRAFProduct Details anti-BRAF Antibody Application Details Handling Images back to top
|Background||Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. Raf-A, a second member of the Raf gene family of serine/threonine protein kinases, exhibits substantial homology to Raf-1 within the kinase domain of the two molecules, but less homology elsewhere. Expression of Raf-B is highly restricted with highest levels in the cerebrum and testis.Synonyms: B-Raf proto-oncogene serine/threonine-protein kinase, BRAF, BRAF1, RAFB1, p94, v-Raf murine sarcoma viral oncogene homolog B1|
|Molecular Weight||approx. 86 kDa|
|Pathways||MAPK Signaling, RTK Signaling, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-BRAF Antibody Target Details BRAF Handling Images back to top
ELISA: 1: 5000approx. 1: 10000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-BRAF Antibody Target Details BRAF Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||DO NOT FREEZE!|
|Storage Comment||Store the antibody undiluted at 2-8 °C.|
ImagesProduct Details anti-BRAF Antibody Target Details BRAF Application Details Handling back to top