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anti-Rat (Rattus) BRAF antibody for Western Blotting

Recommended BRAF Antibody (supplied by: Log in to see )

Antigen
V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) Antibodies
  • 9930012E13Rik
  • AA120551
  • AA387315
  • AA473386
  • B-raf
  • B-Raf
  • B-RAF1
  • b-raf1
  • Braf
  • BRAF
  • Braf-2
  • BRAF1
  • braf1
  • Braf2
  • C-RMIL
  • C87398
  • C230098H17
  • D6Ertd631e
  • HYPT11
  • NS7
  • rafb1
  • RAFB1
  • RMIL
  • sierrabraf
  • Sm-E
  • SME
Epitope
AA 38-230
29
24
23
23
21
17
17
17
15
14
14
13
9
8
7
6
5
4
4
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
376
50
43
6
4
1
1
1
Host
Rabbit
284
89
2
2
Clonality
Polyclonal
Conjugate
This BRAF antibody is un-conjugated
20
20
20
17
17
17
3
3
3
3
3
3
3
3
3
Application
Western Blotting (WB)
248
212
150
83
43
41
33
16
4
4
2
1
1
1
Supplier
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Catalog No. ABIN3043761
$ 240.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.12303223 ABIN3043016 WB Rabbit IgG AA 344-362, Middle Region Log in to see Polyclonal
0.12303223 ABIN258430 FACS IHC (p) IP IHC WB Rabbit IgG Log in to see EP152Y
0.12303223 ABIN1871340 IHC WB Rabbit IgG Log in to see Polyclonal
0.12303223 ABIN2163053 IHC ELISA WB Rabbit IgG Ser601 Log in to see Polyclonal
0.12303223 ABIN2163051 ELISA WB Rabbit IgG Thr753 Log in to see Polyclonal
0.12303223 ABIN2163052 ELISA WB Rabbit IgG Thr735 Log in to see Polyclonal
0.12303223 ABIN1585648 IP IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
0.12303223 ABIN2405722 IHC WB Rabbit IgG Log in to see Polyclonal
0.12303223 ABIN2431557 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.12303223 ABIN2431117 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.12303223 ABIN2425629 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.12303223 ABIN2426109 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.0011806369 ABIN2452570 IF IHC WB Mouse IgG2a Log in to see 3A7
0.0011806369 ABIN1700449 IHC (p) WB Biotin Rabbit IgG pThr753 Log in to see Polyclonal
0.0011806369 ABIN1711466 IHC (p) WB HRP Rabbit IgG pThr753 Log in to see Polyclonal
0.0011806369 ABIN2736363 WB Rabbit IgG Log in to see Polyclonal
0.0011806369 ABIN1713951 IF (p) IHC (p) WB Rabbit IgG pThr753 Log in to see Polyclonal
0.0011806369 ABIN1870020 WB Rabbit IgG pSer445 Log in to see Polyclonal
0.0011806369 ABIN2561461 WB Rabbit IgG pSer728 Log in to see Polyclonal
0.0011806369 ABIN2962049 IF IHC (p) ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal

Similar anti-BRAF Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus)
Western Blotting (WB) 249 Antibodies 38 Antibodies 30 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Luminex Assay (LMNX) 4 Antibodies
Immunoprecipitation (IP) 3 Antibodies 2 Antibodies 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 44 Antibodies 5 Antibodies 5 Antibodies
Immunohistochemistry (IHC) 151 Antibodies 26 Antibodies 18 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 33 Antibodies 11 Antibodies 11 Antibodies
Immunofluorescence (IF) 40 Antibodies 7 Antibodies

General

Antigen V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) Antibodies
Epitope AA 38-230
(29), (24), (23), (23), (21), (17), (17), (17), (15), (14), (14), (13), (9), (8), (7), (6), (5), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(376), (50), (43), (6), (4), (1), (1), (1)
Host Rabbit
(284), (89), (2), (2)
Clonality
Polyclonal
Conjugate This BRAF antibody is un-conjugated
(20), (20), (20), (17), (17), (17), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Application Western Blotting (WB)
(248), (212), (150), (83), (43), (41), (33), (16), (4), (4), (2), (1), (1), (1)
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Product Details anti-BRAF Antibody

Target Details BRAF Application Details Handling Images
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Rabbit IgG polyclonal antibody for Serine/threonine-protein kinase B-raf(BRAF) detection. Tested with WB in Human,Mouse,Rat.
Gene Name: B-Raf proto-oncogene, serine/threonine kinase
Protein Name: Serine/threonine-protein kinase B-raf
Purification Immunogen affinity purified.
Immunogen E.coli-derived human B Raf recombinant protein (Position: A38-V230). Human B Raf shares 81% amino acid (aa) sequence identity with mouse B Raf.
Isotype IgG

Target Details BRAF

Product Details anti-BRAF Antibody Application Details Handling Images back to top
Antigen
Alternative Name BRAF (BRAF Antibody Abstract)
Background BRAF (v-raf murine sarcoma viral oncogene homolog B1) is a human gene that makes a protein called B-Raf. It is a member of the Raf kinase family of growth signal transduction protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. It is mapped to 7q34. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. The BRAF protein is also involved in sending signals inside cells, which are involved in directing cell growth.
Gene ID 673
UniProt P15056
Pathways MAPK Signaling, RTK Signaling, Neurotrophin Signaling Pathway

Application Details

Product Details anti-BRAF Antibody Target Details BRAF Handling Images back to top
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat, The detection limit for B Raf is approximately 0.25 ng/lane under reducing conditions.
Notes: Tested Species: Species with positive results.
Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions For Research Use only

Handling

Product Details anti-BRAF Antibody Target Details BRAF Application Details Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C,4 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Images

Product Details anti-BRAF Antibody Target Details BRAF Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-BRAF antibody (V-Raf Murine Sarcoma Viral Oncogene Homolog B1) (AA 38-230) (ABIN3043761) anti-V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) (AA 38-230) antibody
Western Blotting (WB) image for anti-BRAF antibody (V-Raf Murine Sarcoma Viral Oncogene Homolog B1) (AA 38-230) (ABIN3043761) anti-V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) (AA 38-230) antibody (Image 2)