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anti-Borrelia burgdorferi C-JUN antibody for Western Blotting

Recommended C-JUN Antibody (supplied by: Log in to see )

Antigen
Jun Proto-Oncogene (JUN) Antibodies
  • AP-1
  • AP1
  • B230310J22Rik
  • Bsk
  • BSK
  • BSK/DJNK
  • c-jun
  • c-Jun
  • CG2275
  • CG5680
  • cJun
  • D-JNK
  • d-JRA
  • d-jun
  • d-Jun
  • D-Jun
  • D-jun
  • D-jun/Jra
  • D-junk
  • dAP-1
  • DBSK/JNK
  • DJNK
  • dJNK
  • DJNK/bsk
  • dJRA
  • dJra
  • DJUN
  • DJun
  • djun
  • dJun
  • dJUN
  • Djun
  • dm-Jun
  • Dmel\\CG2275
  • Dmel\\CG5680
  • fj36h07
  • JNK
  • Jnk
  • jnk
  • JNK/SAPK
  • JRA
  • jra
  • jun
  • Jun
  • JUN
  • Junc
  • Junk
  • l(2)46Ef
  • l(2)IA109
  • l(2R)IA109
  • p39
  • SAPKa
  • V
  • wu:fj36h07
  • zgc:65863
Reactivity
Borrelia burgdorferi, Borrelia afzelii
749
410
349
42
36
31
21
17
17
12
12
9
5
5
4
3
2
1
1
Host
Rabbit
678
95
2
2
Clonality
Polyclonal
Conjugate
This C-JUN antibody is un-conjugated
17
17
17
10
10
10
7
7
7
7
7
7
7
7
7
Application
Western Blotting (WB)
587
349
186
100
98
79
75
62
21
20
11
11
8
6
4
3
2
1
1
Supplier
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Catalog No. ABIN964722
$ 369.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.12226487 ABIN964723 WB Rabbit IgG Log in to see Polyclonal

Similar anti-C-JUN Antibodies

Application / Reactivity Borrelia burgdorferi Borrelia afzelii
Western Blotting (WB) 2 Antibodies 2 Antibodies

General

Antigen Jun Proto-Oncogene (JUN) Antibodies
Reactivity Borrelia burgdorferi, Borrelia afzelii
(749), (410), (349), (42), (36), (31), (21), (17), (17), (12), (12), (9), (5), (5), (4), (3), (2), (1), (1)
Host Rabbit
(678), (95), (2), (2)
Clonality
Polyclonal
Conjugate This C-JUN antibody is un-conjugated
(17), (17), (17), (10), (10), (10), (7), (7), (7), (7), (7), (7), (7), (7), (7)
Application Western Blotting (WB)
(587), (349), (186), (100), (98), (79), (75), (62), (21), (20), (11), (11), (8), (6), (4), (3), (2), (1), (1)
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Product Details anti-C-JUN Antibody

Target Details C-JUN Application Details Handling Images
Specificity This product was Protein-A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography. This antibody is specific for Borrelia burgdorferi p39 protein. A BLAST analysis was used to suggest cross-reactivity with p39 from B. burgdorferi and B. afzelii sources based on 100% homology with the immunizing sequence. Partial reactivity is expected against B. garinii sources based on 88-95% homology and to B.spielmanii A14S based on 86-96% homology. Cross-reactivity with p39 from other sources has not been determined.
Cross-Reactivity Borrelia afzelii (B. afzelii)
Characteristics The p39 protein, or Basic membrane protein A, is one of the immunogenic cell membrane components of Borrelia burgdorferi, the spirochete carried by Ixodes ticks. The spirochete migrates from the tick midgut during feeding to its salivary glands and are thus transmitted to the mammal host. This transition may be facilitated by changes in expression of some B. burgdorferi genes. It is believed that expression of the various proteins associated with the spirochete may be regulated by the changes in tick life cycle, changes in conditions during tick feeding (such as temperature, pH, and nutrients) and/or in coordination with the course of infection of the mammal host. BmpA is expressed during the invasion of the spirochete and in the evolution of the arthritis of Lyme disease in mammals. It belongs to the BMP lipoprotein family. The major products of the B. burgdorferi basic membrane protein (bmp) A/B operon that are induced in murine and human joints possess inflammatory properties. Non-lipidated and lipidated versions of BmpA have been shown to induce the pro-inflammatory cytokine TNF-alpha and IL-1ß in human synovial cells. The induction of cytokine responses in synovial cells via activation of the NF-kappaB and p38 MAP kinase pathways could potentially contribute to the genesis of Lyme arthritis. The BmpA outer-surface protein is an important antigen for serodiagnosis of human infection. B. burgdorferi adheres to host extracellular matrix components, including laminin, but may not bind mammalian type I or type IV collagens or fibronectin.
Purification purified
Immunogen MBP-fusion protein corresponding to Borrelia burgdorferi p39 protein.
Immunogen Type: RecombinantProtein
Isotype IgG

Target Details C-JUN

Product Details anti-C-JUN Antibody Application Details Handling Images back to top
Antigen
Alternative Name p39 (JUN Antibody Abstract)
Background The p39 protein, or Basic membrane protein A, is one of the immunogenic cell membrane components of Borrelia burgdorferi, the spirochete carried by Ixodes ticks. The spirochete migrates from the tick midgut during feeding to its salivary glands and are thus transmitted to the mammal host. This transition may be facilitated by changes in expression of some B. burgdorferi genes. It is believed that expression of the various proteins associated with the spirochete may be regulated by the changes in tick life cycle, changes in conditions during tick feeding (such as temperature, pH, and nutrients) and/or in coordination with the course of infection of the mammal host. BmpA is expressed during the invasion of the spirochete and in the evolution of the arthritis of Lyme disease in mammals. It belongs to the BMP lipoprotein family. The major products of the B. burgdorferi basic membrane protein (bmp) A/B operon that are induced in murine and human joints possess inflammatory properties. Non-lipidated and lipidated versions of BmpA have been shown to induce the pro-inflammatory cytokine TNF-α and IL-1ß in human synovial cells. The induction of cytokine responses in synovial cells via activation of the NF-kappaB and p38 MAP kinase pathways could potentially contribute to the genesis of Lyme arthritis. The BmpA outer-surface protein is an important antigen for serodiagnosis of human infection. B. burgdorferi adheres to host extracellular matrix components, including laminin, but may not bind mammalian type I or type IV collagens or fibronectin.
Synonyms: Basic membrane protein A, Borrelia burgdorferi bmpA, immunodominant antigen P39, membrane lipoprotein BmpA
Gene ID 1195220
NCBI Accession NP_212517
UniProt Q45010
Research Area Neurology
Pathways MAPK Signaling, RTK Signaling, WNT Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-C-JUN Antibody Target Details C-JUN Handling Images back to top
Application Notes This protein-A purified antibody has been tested for use in Western blotting. Specific conditions for reactivity should be optimized by the user. Expect a band approximately 35.4 kDa in size corresponding to Borrelia burgdorferi p39 protein by Western blotting in the appropriate cell lysate or extract.
Comment

Gene Name: bmpA, BB_0383

Restrictions For Research Use only

Handling

Product Details anti-C-JUN Antibody Target Details C-JUN Application Details Images back to top
Format Lyophilized
Reconstitution Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 100 µL
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
Expiry Date 12 months

Images

Product Details anti-C-JUN Antibody Target Details C-JUN Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-C-JUN antibody (Jun Proto-Oncogene) (ABIN964722) Western blot showing detection of 0.1 µg of recombinant p39 protein. Lane 1: Molecula...