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|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|Chromatin Immunoprecipitation (ChIP)||3 Antibodies||4 Antibodies|
|ELISA||72 Antibodies||93 Antibodies|
|Enzyme Immunoassay (EIA)||2 Antibodies||4 Antibodies|
|Flow Cytometry (FACS)||2 Antibodies|
|Immunochromatography (IC)||4 Antibodies|
|Immunocytochemistry (ICC)||12 Antibodies|
|Immunofluorescence (IF)||55 Antibodies|
|Immunofluorescence (Cultured Cells) (IF (cc))||13 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||93 Antibodies|
|Immunohistochemistry (IHC)||202 Antibodies|
|Immunohistochemistry (Formalin-fixed Sections) (IHC (f))||1 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||71 Antibodies|
|Immunoprecipitation (IP)||48 Antibodies|
|Western Blotting (WB)||293 Antibodies|
|Dot Blot (DB)||19 Antibodies|
|Electrophoretic Mobility-Shift Assay (EMSA)||1 Antibodies|
|Antigen||Jun Proto-Oncogene (JUN) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This C-JUN antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
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Product Details anti-C-JUN AntibodyTarget Details C-JUN Application Details Handling Images
|Specificity||Antibody detects endogenous levels of AP-1 / c-Jun protein (region surrounding Asn85).|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Purification||The antibody was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen|
|Purity||> 95 % pure by SDS-PAGE|
Target Details C-JUNProduct Details anti-C-JUN Antibody Application Details Handling Images back to top
|Alternative Name||AP-1 / C-Jun (JUN Antibody Abstract)|
|Background||The c-Jun proto-oncogene was first identified as the cellular homolog of the avian sarcoma virus v-Jun oncogene. The c-Jun protein, along with c-Fos, is a component of the AP-1 transcriptional complex. c-Jun can form either Jun/Jun homodimers or Jun/Fos heterodimers via the leucine repeats in both proteins. Homo- and heterodimers bind to the TGACTCA consensus sequence present in numerous promoters and initially identified as the phorbol ester tumor promoter response element (TRE). Two additional genes, Jun B and Jun D, have been shown to be almost identical to c-Jun in their C-terminal regions, which are involved in dimerization and DNA binding, whereas their N-terminal domains, which are involved in transcriptional activation, diverge. All three form heterodimers among themselves and with c-Fos and other members of the Fos gene family.Synonyms: Activator protein 1, Proto-oncogene c-jun, Transcription factor AP1, V-jun avian sarcoma virus 17 oncogene homolog, p39|
|Molecular Weight||approx. 43, 48 kDa|
|Research Area||Transcription Factors|
|Pathways||MAPK Signaling, RTK Signaling, WNT Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway|
Application DetailsProduct Details anti-C-JUN Antibody Target Details C-JUN Handling Images back to top
ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200. IP: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-C-JUN Antibody Target Details C-JUN Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS), pH 7.2., 0.05 % Sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
ImagesProduct Details anti-C-JUN Antibody Target Details C-JUN Application Details Handling back to top