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|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|Chromatin Immunoprecipitation (ChIP)||3 Antibodies||4 Antibodies|
|ELISA||72 Antibodies||93 Antibodies|
|Enzyme Immunoassay (EIA)||2 Antibodies||4 Antibodies|
|Flow Cytometry (FACS)||2 Antibodies|
|Immunochromatography (IC)||4 Antibodies|
|Immunocytochemistry (ICC)||12 Antibodies|
|Immunofluorescence (IF)||55 Antibodies|
|Immunofluorescence (Cultured Cells) (IF (cc))||11 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||79 Antibodies|
|Immunohistochemistry (IHC)||202 Antibodies|
|Immunohistochemistry (Formalin-fixed Sections) (IHC (f))||1 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||71 Antibodies|
|Immunoprecipitation (IP)||48 Antibodies|
|Western Blotting (WB)||293 Antibodies|
|Dot Blot (DB)||19 Antibodies|
|Electrophoretic Mobility-Shift Assay (EMSA)||1 Antibodies|
|Antigen||Jun Proto-Oncogene (JUN) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This C-JUN antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-C-JUN AntibodyTarget Details C-JUN Application Details Handling Images
|Specificity||This antibody detects endogenous levels of c-Jun protein. (region surrounding Ile245)|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Purification||The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen|
|Purity||> 95 % pure by SDS-PAGE|
Target Details C-JUNProduct Details anti-C-JUN Antibody Application Details Handling Images back to top
|Alternative Name||AP-1 / C-Jun (JUN Antibody Abstract)|
|Background||Genes belonging to the Jun and Fos oncogene families encode nuclear proteins that are found to be associated with a number of transcriptional complexes. The c-Jun protein is a major component of the transcription factor AP-1 originally shown to mediate phorbol ester tumor promoter (TPA)-induced expression of responsive genes through the TPA- response element (TRE). The Jun proteins form homo- and heterodimers which bind the TRE, while Fos proteins are active only as heterodimers with any of the Jun proteins. Fos/Jun heterodimers have a much higher affinity for the TRE than Jun homodimers. Ha-Ras augments c-Jun activity and stimulates phosphorylation of its activation domain. An inhibitor of Fos/Jun function, termed IP-1, associates with Fos and Jun and is inactivated upon phosphorylation induced by the cAMP-dependent protein kinase A (PKA).Synonyms: Activator protein 1, Proto-oncogene c-jun, Transcription factor AP1, V-jun avian sarcoma virus 17 oncogene homolog, p39|
|Molecular Weight||approx. 43, 48 kDa|
|Research Area||Transcription Factors|
|Pathways||MAPK Signaling, RTK Signaling, WNT Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway|
Application DetailsProduct Details anti-C-JUN Antibody Target Details C-JUN Handling Images back to top
ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-C-JUN Antibody Target Details C-JUN Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS), pH 7.2., 15 mM sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||DO NOT FREEZE!|
|Storage Comment||Store the antibody undiluted at 2-8 °C.|
ImagesProduct Details anti-C-JUN Antibody Target Details C-JUN Application Details Handling back to top