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|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|Chromatin Immunoprecipitation (ChIP)||2 Antibodies||3 Antibodies|
|ELISA||67 Antibodies||87 Antibodies|
|Enzyme Immunoassay (EIA)||2 Antibodies||4 Antibodies|
|Flow Cytometry (FACS)||3 Antibodies|
|Immunochromatography (IC)||4 Antibodies|
|Immunocytochemistry (ICC)||14 Antibodies|
|Immunofluorescence (IF)||53 Antibodies|
|Immunofluorescence (Cultured Cells) (IF (cc))||11 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||79 Antibodies|
|Immunohistochemistry (IHC)||203 Antibodies|
|Immunohistochemistry (Formalin-fixed Sections) (IHC (f))||1 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||2 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||73 Antibodies|
|Immunoprecipitation (IP)||49 Antibodies|
|Western Blotting (WB)||294 Antibodies|
|Dot Blot (DB)||18 Antibodies|
|Electrophoretic Mobility-Shift Assay (EMSA)||1 Antibodies|
|Antigen||Jun Proto-Oncogene (JUN) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This C-JUN antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
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Product Details anti-C-JUN AntibodyTarget Details C-JUN Application Details Handling Images
|Cross-Reactivity (Details)||Predicted to cross-react with pig, based on sequence identity.|
|Purification||Tissue culture supernatant|
|Immunogen||A synthetic peptide corresponding to N-terminal residues of human c-Jun was used as immunogen.|
Target Details C-JUNProduct Details anti-C-JUN Antibody Application Details Handling Images back to top
|Alternative Name||AP-1 / c-Jun (JUN Antibody Abstract)|
C-Jun is a component of the transcription factor AP-1 (1). The transcriptional activity ofc-Jun is regulated by phosphorylation at serines 63 and 73 (1,2). Extracellular signals,including growth factors, transforming oncoproteins and UV light, stimulatephosphorylation of c-Jun at Ser63/73 and activate c-Jun-dependent transcription (3,4). Alternate Names: anti-AP1 antibody, anti-Activator Protein 1 antibody, anti-Enhancer Binding Protein AP1antibody, anti-JUN antibody, anti-JUN protein antibody, anti-JUNC antibody, anti-JunActivation Domain Binding Protein antibody, anti-Jun oncogene antibody, anti-Protooncogene cJun antibody, anti-Transcription Factor AP1 antibody, anti-V jun aviansarcoma virus 17 oncogene homolog antibody, anti-p39 antibody, anti-vJun AvianSarcoma Virus 17 Oncogene Homolog antibody.
Gene Symbol: JUN
|Gene ID||3725, 16476, 24516|
|Pathways||MAPK Signaling, RTK Signaling, WNT Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway|
Application DetailsProduct Details anti-C-JUN Antibody Target Details C-JUN Handling Images back to top
This product is useful for: Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunoprecipitation. In Western blot this antibody detects a band at approximately 43kDa. Flow Cytometry was reported in scientific literature.
Recommended dilutions: Flow Cytometry 1:10-1:1000, Immunocytochemistry/Immunofluorescence 1:250, Immunohistochemistry 1:10-1:2000, Immunohistochemistry-Paraffin 1:250, Immunoprecipitation 1:40, Western Blot 1:2000
Protocol specific for c-Jun Antibody Immunohistochemistry Protocol for Paraffin-embedded Tissues
1. Solutions and reagents1.
. Ethanol, anhydrous denatured, histological grade (100 %, 95 %, 70 %)1.
. Washing buffer:TBST washing buffer: 1XTBS/0.1 % Tween-20To prepare stock solution of 10X TBS: add 24.2 g Trizma base and 80 g sodium chloride to 1L of dH2O. Adjust pH to 7.6.Working solution. 1XTBST/0.1 % Tween-20: add 100ml 10XTBS to 900 mL dH2O. Add 1 mL Tween-20 and mix well.1.
. Distilled water (dH2O)1.
. Antigen Retrieval Solution:0.01M Sodium Citrate
|Restrictions||For Research Use only|
HandlingProduct Details anti-C-JUN Antibody Target Details C-JUN Application Details Images back to top
|Buffer||PBS 49 % , Glycerol 50 % , BSA 0.05 % , Sodium Azide|
|Precaution of Use||WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.|
|Handling Advice||Avoid freeze-thaw cycles|
|Storage Comment||-20 °C.|
ImagesProduct Details anti-C-JUN Antibody Target Details C-JUN Application Details Handling back to top