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anti-Rat (Rattus) C-JUN antibody for Flow Cytometry

Recommended C-JUN Antibody (supplied by: Log in to see )

Antigen
Jun Proto-Oncogene (JUN) Antibodies
  • AP-1
  • AP1
  • B230310J22Rik
  • Bsk
  • BSK
  • BSK/DJNK
  • c-jun
  • c-Jun
  • CG2275
  • CG5680
  • cJun
  • D-JNK
  • d-JRA
  • d-jun
  • d-Jun
  • D-Jun
  • D-jun
  • D-jun/Jra
  • D-junk
  • dAP-1
  • DBSK/JNK
  • DJNK
  • dJNK
  • DJNK/bsk
  • dJRA
  • dJra
  • DJUN
  • DJun
  • djun
  • dJun
  • dJUN
  • Djun
  • dm-Jun
  • Dmel\\CG2275
  • Dmel\\CG5680
  • fj36h07
  • JNK
  • Jnk
  • jnk
  • JNK/SAPK
  • JRA
  • jra
  • jun
  • Jun
  • JUN
  • Junc
  • Junk
  • l(2)46Ef
  • l(2)IA109
  • l(2R)IA109
  • p39
  • SAPKa
  • V
  • wu:fj36h07
  • zgc:65863
Epitope
N-Term
77
73
60
42
41
41
41
34
23
20
20
19
13
13
13
13
12
11
8
8
8
8
8
6
6
6
5
5
5
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
804
440
395
42
36
31
22
17
17
12
12
9
5
5
4
3
2
2
2
1
Host
Rabbit
711
117
2
2
1
Clonality
Monoclonal
Conjugate
This C-JUN antibody is un-conjugated
18
18
18
11
10
10
8
8
7
7
7
7
7
7
7
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
641
381
202
130
105
79
75
73
28
22
11
11
8
6
4
3
2
1
1
Supplier
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Available images

Catalog No. ABIN189128
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010463644 ABIN2705622 ChIP FACS IP IHC WB Rabbit Center Log in to see Polyclonal
0.0010463644 ABIN4285648 ELISA FACS ICC IF IHC WB Mouse IgG1 Log in to see 5B1

Similar anti-C-JUN Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
Chromatin Immunoprecipitation (ChIP) 2 Antibodies 3 Antibodies
ELISA 67 Antibodies 87 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 4 Antibodies
Flow Cytometry (FACS) 3 Antibodies
Immunochromatography (IC) 4 Antibodies
Immunocytochemistry (ICC) 14 Antibodies
Immunofluorescence (IF) 53 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 11 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 79 Antibodies
Immunohistochemistry (IHC) 203 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 73 Antibodies
Immunoprecipitation (IP) 49 Antibodies
Western Blotting (WB) 294 Antibodies
Dot Blot (DB) 18 Antibodies
Electrophoretic Mobility-Shift Assay (EMSA) 1 Antibodies

General

Antigen Jun Proto-Oncogene (JUN) Antibodies
Epitope N-Term
(77), (73), (60), (42), (41), (41), (41), (34), (23), (20), (20), (19), (13), (13), (13), (13), (12), (11), (8), (8), (8), (8), (8), (6), (6), (6), (5), (5), (5), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(804), (440), (395), (42), (36), (31), (22), (17), (17), (12), (12), (9), (5), (5), (4), (3), (2), (2), (2), (1)
Host Rabbit
(711), (117), (2), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This C-JUN antibody is un-conjugated
(18), (18), (18), (11), (10), (10), (8), (8), (7), (7), (7), (7), (7), (7), (7), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(641), (381), (202), (130), (105), (79), (75), (73), (28), (22), (11), (11), (8), (6), (4), (3), (2), (1), (1)
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Product Details anti-C-JUN Antibody

Target Details C-JUN Application Details Handling Images
Cross-Reactivity (Details) Predicted to cross-react with pig, based on sequence identity.
Purification Tissue culture supernatant
Immunogen A synthetic peptide corresponding to N-terminal residues of human c-Jun was used as immunogen.
Clone E254
Isotype IgG

Target Details C-JUN

Product Details anti-C-JUN Antibody Application Details Handling Images back to top
Antigen
Alternative Name AP-1 / c-Jun (JUN Antibody Abstract)
Background C-Jun is a component of the transcription factor AP-1 (1). The transcriptional activity ofc-Jun is regulated by phosphorylation at serines 63 and 73 (1,2). Extracellular signals,including growth factors, transforming oncoproteins and UV light, stimulatephosphorylation of c-Jun at Ser63/73 and activate c-Jun-dependent transcription (3,4). Alternate Names: anti-AP1 antibody, anti-Activator Protein 1 antibody, anti-Enhancer Binding Protein AP1antibody, anti-JUN antibody, anti-JUN protein antibody, anti-JUNC antibody, anti-JunActivation Domain Binding Protein antibody, anti-Jun oncogene antibody, anti-Protooncogene cJun antibody, anti-Transcription Factor AP1 antibody, anti-V jun aviansarcoma virus 17 oncogene homolog antibody, anti-p39 antibody, anti-vJun AvianSarcoma Virus 17 Oncogene Homolog antibody.
Gene Symbol: JUN
Gene ID 3725, 16476, 24516
Pathways MAPK Signaling, RTK Signaling, WNT Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-C-JUN Antibody Target Details C-JUN Handling Images back to top
Application Notes This product is useful for: Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunoprecipitation. In Western blot this antibody detects a band at approximately 43kDa. Flow Cytometry was reported in scientific literature.
Recommended dilutions: Flow Cytometry 1:10-1:1000, Immunocytochemistry/Immunofluorescence 1:250, Immunohistochemistry 1:10-1:2000, Immunohistochemistry-Paraffin 1:250, Immunoprecipitation 1:40, Western Blot 1:2000
Protocol Protocol specific for c-Jun Antibody Immunohistochemistry Protocol for Paraffin-embedded Tissues
1. Solutions and reagents1.
. Xylene1.
. Ethanol, anhydrous denatured, histological grade (100 %, 95 %, 70 %)1.
. Washing buffer:TBST washing buffer: 1XTBS/0.1 % Tween-20To prepare stock solution of 10X TBS: add 24.2 g Trizma base and 80 g sodium chloride to 1L of dH2O. Adjust pH to 7.6.Working solution. 1XTBST/0.1 % Tween-20: add 100ml 10XTBS to 900 mL dH2O. Add 1 mL Tween-20 and mix well.1.
. Distilled water (dH2O)1.
. Antigen Retrieval Solution:0.01M Sodium Citrate
Restrictions For Research Use only

Handling

Product Details anti-C-JUN Antibody Target Details C-JUN Application Details Images back to top
Format Liquid
Buffer PBS 49 % , Glycerol 50 % , BSA 0.05 % , Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment -20 °C.

Images

Product Details anti-C-JUN Antibody Target Details C-JUN Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-C-JUN antibody (Jun Proto-Oncogene) (N-Term) (ABIN189128) Western blot analysis on NIH3T3 cell lysate using anti-c-Jun (N-term) RabMAb (ABIN189...
Immunohistochemistry (IHC) image for anti-C-JUN antibody (Jun Proto-Oncogene) (N-Term) (ABIN189128) Immunohistochemical analysis of paraffin-embedded skin carcinoma using anti-c-Jun (N-...