You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Chicken Calnexin antibody for ELISA

Recommended Calnexin Antibody (supplied by: Log in to see )

Antigen
Calnexin (CANX) Antibodies
  • 1110069N15Rik
  • AI988026
  • CANX
  • canx
  • CG11958
  • clgn
  • clnx
  • Cnx
  • CNX
  • cnx
  • D11Ertd153e
  • Dmel\CG11958
  • IP90
  • P90
  • PP90
  • wu:fe06b12
  • zgc:63524
Epitope
C-Term
100
27
19
13
12
10
5
4
4
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus), Chicken, Primate
315
179
172
99
92
87
86
84
83
82
82
81
77
52
37
21
12
8
5
4
4
4
4
4
2
2
1
1
1
Host
Rabbit
222
88
25
Clonality
Polyclonal
Conjugate
This Calnexin antibody is un-conjugated
12
10
10
8
7
6
6
5
5
5
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
1
1
1
1
1
1
1
Application
Fluorescence Microscopy (FM), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
310
208
163
146
129
109
84
72
11
9
6
3
3
2
2
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Catalog No. ABIN1607882
$ 369.00
Plus shipping costs $45.00 and $21.00 dry ice
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0012117105 ABIN4287258 ELISA ICC IF IHC WB Rabbit C-Term Log in to see Polyclonal

Similar anti-Calnexin Antibodies

Application / Reactivity Human Chicken Mouse (Murine) Rat (Rattus) Primate
Western Blotting (WB) 292 Antibodies 84 Antibodies 165 Antibodies 160 Antibodies 9 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Immunoprecipitation (IP) 99 Antibodies 57 Antibodies 74 Antibodies 75 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 19 Antibodies 123 Antibodies 63 Antibodies 62 Antibodies

General

Antigen Calnexin (CANX) Antibodies
Epitope C-Term
(100), (27), (19), (13), (12), (10), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus), Chicken, Primate
(315), (179), (172), (99), (92), (87), (86), (84), (83), (82), (82), (81), (77), (52), (37), (21), (12), (8), (5), (4), (4), (4), (4), (4), (2), (2), (1), (1), (1)
Host Rabbit
(222), (88), (25)
Clonality
Polyclonal
Conjugate This Calnexin antibody is un-conjugated
(12), (10), (10), (8), (7), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1)
Application Fluorescence Microscopy (FM), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(310), (208), (163), (146), (129), (109), (84), (72), (11), (9), (6), (3), (3), (2), (2), (1)
Supplier Log in to see

Product Details anti-Calnexin Antibody

Target Details Calnexin Application Details Handling Images
Cross-Reactivity (Details) Cross reactivity with calnexin from other sources has not been determined.
Purity Anti-Calnexin was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is specific towards calnexin. A BLAST analysis was used to suggest cross-reactivity with Human, Mouse, Rat, Primate and Chicken based on 100 % sequence homology.
Endotoxin Level Low Endotoxin : No
Immunogen Calnexin affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminus of human Calnexin.
Immunogen Type: Peptide

Target Details Calnexin

Product Details anti-Calnexin Antibody Application Details Handling Images back to top
Antigen
Alternative Name Calnexin (CANX Antibody Abstract)
Background Calnexin, also referred to as IP90, p88 and p90, is an ~90 kDa integral membrane protein of the endoplasmic reticulum (ER). Many resident ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into multisubunit proteins. Studies indicate that calnexin associates with the major histocompatability complex (MHC) class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin, but not with completed receptor complexes. It has been shown that calnexin is a chaperone that retains incompletely or improperly folded proteins in the ER. The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence, is present at the carboxy-terminus of soluble ER resident proteins such as GRP 78 and GRP 94 and protein disulfide isomerase. Integral membrane ER resident proteins, like calnexin, often lack this KDEL sequence but contain positively charged cytosolic residues that ensure ER retention. Calnexin contains a large ER luminal domain (461 amino acids), a transmembrane segment (22 amino acids), and a cytoplasmic tail (89 amino acids). These features distinguish calnexin from soluble ER chaperones that cannot interact with the transmembrane and cytosolic domains of integral membrane proteins. The amino acid sequence of calnexin is highly conserved among species, and shares regions of high sequence homology with calreticulin. Anti-Calnexin antibody is ideal for researchers interested in ER Markers, Neuroscience, and Vision research. Synonyms: calnexin, CNX, IP90FLJ26570, Major histocompatibility complex class I antigen-binding protein p88, p90, P90
Gene ID 821
NCBI Accession NP_001019820
UniProt P27824
Pathways MAPK Signaling

Application Details

Product Details anti-Calnexin Antibody Target Details Calnexin Handling Images back to top
Application Notes Anti-Calnexin antibody is useful for ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~67.7 kDa corresponding to the appropriate cell lysate or extract.
ELISA Dilution: 1:20.000 - 1:60.000
Immunohistochemistry Dilution: 1:100-1:500
IF Microscopy Dilution: 1:100-1:500
Western Blot Dilution: 0.2 µg/mL
Restrictions For Research Use only

Handling

Product Details anti-Calnexin Antibody Target Details Calnexin Application Details Images back to top
Format Liquid
Concentration 0.34 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 50 % (v/v) Glycerol
Storage 4 °C/-20 °C
Storage Comment Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use.
Expiry Date Expiration date is six (6) months from date of opening.