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anti-Human Calnexin antibody for BioImaging

Recommended Calnexin Antibody (supplied by: Log in to see )

Antigen
Calnexin (CANX) Antibodies
  • 1110069N15Rik
  • AI988026
  • CANX
  • canx
  • CG11958
  • clgn
  • clnx
  • Cnx
  • CNX
  • cnx
  • D11Ertd153e
  • Dmel\CG11958
  • IP90
  • P90
  • PP90
  • wu:fe06b12
  • zgc:63524
Epitope
AA 116-301
83
24
10
7
5
4
4
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
220
126
120
91
73
68
67
66
65
64
63
63
60
37
37
5
4
4
4
4
4
4
2
1
1
1
1
Host
Mouse
189
27
24
Clonality
Monoclonal
Conjugate
This Calnexin antibody is un-conjugated
7
7
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
1
1
1
1
1
1
1
1
1
Application
BioImaging (BI), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
217
134
106
89
78
69
43
38
11
9
5
3
1
1
1
Supplier
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Catalog No. ABIN968018
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008676631 ABIN968017 BI IP IHC WB Mouse IgG1 AA 116-301 Log in to see 37-Calnexin

Top referenced anti-Calnexin antibody for BioImaging

Similar anti-Calnexin Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
BioImaging (BI) 2 Antibodies 2 Antibodies
ELISA 18 Antibodies 23 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 2 Antibodies
Flow Cytometry (FACS) 20 Antibodies 20 Antibodies
Fluorescence Microscopy (FM) 1 Antibodies 1 Antibodies
Immunochromatography (IC) 2 Antibodies 2 Antibodies
Immunocytochemistry (ICC) 57 Antibodies 59 Antibodies
Immunofluorescence (IF) 82 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 84 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 12 Antibodies
Immunoprecipitation (IP) 45 Antibodies
Western Blotting (WB) 108 Antibodies

General

Antigen Calnexin (CANX) Antibodies
Epitope AA 116-301
(83), (24), (10), (7), (5), (4), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(220), (126), (120), (91), (73), (68), (67), (66), (65), (64), (63), (63), (60), (37), (37), (5), (4), (4), (4), (4), (4), (4), (2), (1), (1), (1), (1)
Host Mouse
(189), (27), (24)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Calnexin antibody is un-conjugated
(7), (7), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application BioImaging (BI), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(217), (134), (106), (89), (78), (69), (43), (38), (11), (9), (5), (3), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-Calnexin Antibody

Target Details Calnexin Application Details Handling References for anti-Calnexin Antibody (ABIN968018) Images
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. This antibody has been developed and certified for the bioimaging application. However, a routine bioimaging test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.
3. Triton is a trademark of the Dow Chemical Company.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
6. Please refer to us for technical protocols.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Human Calnexin aa. 116-301
Clone 37-Calnexin
Isotype IgG1

Target Details Calnexin

Product Details anti-Calnexin Antibody Application Details Handling References for anti-Calnexin Antibody (ABIN968018) Images back to top
Antigen
Alternative Name Calnexin (CANX Antibody Abstract)
Background Calnexin is a 90 kDa integral membrane protein located primarily in the ER. It contains a long N-terminal calcium-binding domain that extends into the lumen of the ER and a short, acidic cytosolic domain. Calnexin associates with several cell surface proteins as they pass through the ER. Since it also forms complexes with other resident ER proteins involved in the Ca2+-dependent retention of proteins, calnexin may act as a chaperone. The amino acid sequence of calnexin is highly conserved among species and is similar in sequence to calreticulin, another Ca2+-binding protein found in the ER. Neither calnexin nor calreticulin contain the calcium-binding motif, known as the E-F hand, found in the calmodulin family of proteins.
Molecular Weight 90 kDa
Pathways MAPK Signaling

Application Details

Product Details anti-Calnexin Antibody Target Details Calnexin Handling References for anti-Calnexin Antibody (ABIN968018) Images back to top
Application Notes Bioimaging
1. Seed the cells in appropriate culture medium at ~10,000 cells per well in an 96-well Imaging Plate and culture overnight.
2. Remove the culture medium from the wells, and fix the cells by adding 100 myl of Fixation Buffer to each well. Incubate for 10 minutes at room temperature (RT).
3. Remove the fixative from the wells, and permeabilize the cells using either 90% methanol, or Triton™ X-100: a. Add 100 myl of -20°C 90% methanol to each well and incubate for 5 minutes at RT. OR b. Add 100 myl of 0.1% Triton™ X-100 to each well and incubate for 5 minutes at RT.
4. Remove the permeabilization buffer, and wash the wells twice with 100 myl of 1× PBS.
5. Remove the PBS, and block the cells by adding 100 myl of to each well. Incubate for 30 minutes at RT.
6. Remove the blocking buffer and add 50 myl of the optimally titrated primary antibody (diluted in Stain Buffer) to each well, and incubate for 1 hour at RT.
7. Remove the primary antibody, and wash the wells three times with 100 myl of 1× PBS.
8. Remove the PBS, and add the second step reagent at its optimally titrated concentration in 50 myl to each well, and incubate in the dark for 1 hour at RT.
9. Remove the second step reagent, and wash the wells three times with 100 myl of 1× PBS.
10. Remove the PBS, and counter-stain the nuclei by adding 200 myl per well of 2 myg/ml Hoechst 33342 in 1× PBS to each well at least 15 minutes before imaging.
11. View and analyze the cells on an appropriate imaging instrument.
Comment

Related Products: ABIN967389, ABIN968535

Restrictions For Research Use only

Handling

Product Details anti-Calnexin Antibody Target Details Calnexin Application Details References for anti-Calnexin Antibody (ABIN968018) Images back to top
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20°C.

References for anti-Calnexin Antibody (ABIN968018)

Product Details anti-Calnexin Antibody Target Details Calnexin Application Details Handling Images back to top
Product cited in:

Nagahama, Suzuki, Hamada, Hatsuzawa, Tani, Yamamoto, Tagaya: "SVIP is a novel VCP/p97-interacting protein whose expression causes cell vacuolation." in: Molecular biology of the cell, Vol. 14, Issue 1, pp. 262-73, 2003

Gkantiragas, Brügger, Stüven, Kaloyanova, Li, Löhr, Lottspeich, Wieland, Helms: "Sphingomyelin-enriched microdomains at the Golgi complex." in: Molecular biology of the cell, Vol. 12, Issue 6, pp. 1819-33, 2001

Lee, Kim, Choi, Mehl, Jung, Gil, Rowe, Park: "CD99 expression is positively regulated by Sp1 and is negatively regulated by Epstein-Barr virus latent membrane protein 1 through nuclear factor-kappaB." in: Blood, Vol. 97, Issue 11, pp. 3596-604, 2001

Tjoelker, Seyfried, Eddy, Byers, Shows, Calderon, Schreiber, Gray: "Human, mouse, and rat calnexin cDNA cloning: identification of potential calcium binding motifs and gene localization to human chromosome 5." in: Biochemistry, Vol. 33, Issue 11, pp. 3229-36, 1994

Images

Product Details anti-Calnexin Antibody Target Details Calnexin Application Details Handling References for anti-Calnexin Antibody (ABIN968018) back to top
Supplier Images
Western Blotting (WB) image for anti-Calnexin antibody (CANX) (AA 116-301) (ABIN968018) Western blot analysis of Calnexin on a HeLa lysate. Lane 1: 1:1000, lane 2: 1:2000, l...
Immunofluorescence (IF) image for anti-Calnexin antibody (CANX) (AA 116-301) (ABIN968018) Immunofluorescent staining of A549 (ATCC CCL-185) cells. Cells were seeded in a 96 we...
 image for anti-Calnexin antibody (CANX) (AA 116-301) (ABIN968018) anti-Calnexin (CANX) (AA 116-301) antibody (Image 3)