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|Application / Reactivity||Human|
|Enzyme Immunoassay (EIA)||6 Antibodies|
|Flow Cytometry (FACS)||6 Antibodies|
|Immunochromatography (IC)||8 Antibodies|
|Immunocytochemistry (ICC)||25 Antibodies|
|Immunofluorescence (IF)||64 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||46 Antibodies|
|Immunohistochemistry (IHC)||103 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||7 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||60 Antibodies|
|Immunoprecipitation (IP)||57 Antibodies|
|Intracellular Flow Cytometry (ICFC)||2 Antibodies|
|Proximity Ligation Assay (PLA)||2 Antibodies|
|Western Blotting (WB)||329 Antibodies|
|Antigen||Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9) Antibodies|
|Conjugate||This Caspase 9 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|5 references available|
|Supplier||Log in to see|
Product Details anti-Caspase 9 AntibodyTarget Details Caspase 9 Application Details Handling References for anti-Caspase 9 Antibody (ABIN358092) Images
|Specificity||This antibody detects Caspase-9 pSer196.|
|Purification||Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Immunogen||This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S196 of human caspase 9.|
Target Details Caspase 9Product Details anti-Caspase 9 Antibody Application Details Handling References for anti-Caspase 9 Antibody (ABIN358092) Images back to top
|Alternative Name||Caspase-9 (CASP9 Antibody Abstract)|
|Background||Caspase 9 is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspase APAF1, this step is thought to be one of the earliest in the caspase activation cascade.Synonyms: APAF-3, APAF3, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, CASP-9, CASP9, ICE-LAP6, ICE-like apoptotic protease 6, MCH6|
|Molecular Weight||46281 Da|
|Gene ID||842, 9606|
|Research Area||Apoptosis/Necrosis, Cancer|
|Pathways||MAPK Signaling, RTK Signaling, Apoptosis, Caspase Cascade in Apoptosis, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-Caspase 9 Antibody Target Details Caspase 9 Handling References for anti-Caspase 9 Antibody (ABIN358092) Images back to top
ELISA: 1/1,000. Western Blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Caspase 9 Antibody Target Details Caspase 9 Application Details References for anti-Caspase 9 Antibody (ABIN358092) Images back to top
|Buffer||PBS with 0.09 % (W/V) Sodium Azide as preservative.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
References for anti-Caspase 9 Antibody (ABIN358092)Product Details anti-Caspase 9 Antibody Target Details Caspase 9 Application Details Handling Images back to top
Martin, Allan, Lickrish et al.: "Protein kinase A regulates caspase-9 activation by Apaf-1 downstream of cytochrome c." in: The Journal of biological chemistry, Vol. 280, Issue 15, pp. 15449-55, 2005 (PubMed).
Cornelis, Bruynooghe, Van Loo et al.: "Apoptosis of hematopoietic cells induced by growth factor withdrawal is associated with caspase-9 mediated cleavage of Raf-1." in: Oncogene, Vol. 24, Issue 9, pp. 1552-62, 2005 (PubMed).
Mohammad, Wang, Aboukameel et al.: "Preclinical studies of a nonpeptidic small-molecule inhibitor of Bcl-2 and Bcl-X(L) [(-)-gossypol] against diffuse large cell lymphoma." in: Molecular cancer therapeutics, Vol. 4, Issue 1, pp. 13-21, 2005 (PubMed).
Raina, Pandey, Ahmad et al.: "c-Abl tyrosine kinase regulates caspase-9 autocleavage in the apoptotic response to DNA damage." in: The Journal of biological chemistry, Vol. 280, Issue 12, pp. 11147-51, 2005 (PubMed).
Tacconi, Perri, Balestrieri et al.: "Increased caspase activation in peripheral blood mononuclear cells of patients with Alzheimer's disease." in: Experimental neurology, Vol. 190, Issue 1, pp. 254-62, 2004 (PubMed).
ImagesProduct Details anti-Caspase 9 Antibody Target Details Caspase 9 Application Details Handling References for anti-Caspase 9 Antibody (ABIN358092) back to top