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anti-Human Caspase 9 antibody for Enzyme Immunoassay

Recommended Caspase 9 Antibody (supplied by: Log in to see )

Antigen
Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9) Antibodies
  • AI115399
  • APAF-3
  • APAF3
  • Apaf3
  • AW493809
  • CASP-9
  • Casp-9-CTD
  • CASP9
  • Casp9_v1
  • Caspase-9
  • Ice-Lap6
  • ICE-LAP6
  • MCH6
  • Mch6
  • PPP1R56
Epitope
pSer196
34
22
21
19
18
16
13
13
13
13
12
11
11
9
8
7
7
7
6
5
5
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
413
118
115
19
13
9
5
4
4
2
2
2
1
1
1
1
Host
Rabbit
419
47
1
Clonality
Polyclonal
Conjugate
This Caspase 9 antibody is un-conjugated
11
10
7
4
4
4
4
4
4
4
4
4
3
3
3
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
390
161
129
78
70
68
45
35
13
13
8
5
2
2
2
Supplier
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Catalog No. ABIN358092
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008867729 ABIN360397 EIA WB Rabbit Ig Middle Region Log in to see Polyclonal 3
0.0008867729 ABIN499577 EIA WB Rabbit IgG Intermediate Domain 1 Log in to see Polyclonal 4
0.0008867729 ABIN499579 EIA IF WB Rabbit IgG Intermediate Domain 2 Log in to see Polyclonal 1
0.0008867729 ABIN615299 EIA IF IHC (p) WB Rabbit AA 299-318, N-Term Log in to see Polyclonal
0.0008867729 ABIN2717104 EIA Rabbit IgG Log in to see Polyclonal

Top referenced anti-Caspase 9 antibody for Enzyme Immunoassay

Similar anti-Caspase 9 Antibodies

Application / Reactivity Human
ELISA 138 Antibodies
Enzyme Immunoassay (EIA) 6 Antibodies
Flow Cytometry (FACS) 8 Antibodies
Immunochromatography (IC) 8 Antibodies
Immunocytochemistry (ICC) 28 Antibodies
Immunofluorescence (IF) 67 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 45 Antibodies
Immunohistochemistry (IHC) 117 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 11 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 74 Antibodies
Immunoprecipitation (IP) 68 Antibodies
Intracellular Flow Cytometry (ICFC) 2 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Western Blotting (WB) 342 Antibodies

General

Antigen Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9) Antibodies
Epitope pSer196
(34), (22), (21), (19), (18), (16), (13), (13), (13), (13), (12), (11), (11), (9), (8), (7), (7), (7), (6), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(413), (118), (115), (19), (13), (9), (5), (4), (4), (2), (2), (2), (1), (1), (1), (1)
Host Rabbit
(419), (47), (1)
Clonality
Polyclonal
Conjugate This Caspase 9 antibody is un-conjugated
(11), (10), (7), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(390), (161), (129), (78), (70), (68), (45), (35), (13), (13), (8), (5), (2), (2), (2)
Pubmed 5 references available
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Product Details anti-Caspase 9 Antibody

Target Details Caspase 9 Application Details Handling References for anti-Caspase 9 Antibody (ABIN358092) Images
Specificity This antibody detects Caspase-9 pSer196.
Purification Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S196 of human caspase 9.
Isotype Ig

Target Details Caspase 9

Product Details anti-Caspase 9 Antibody Application Details Handling References for anti-Caspase 9 Antibody (ABIN358092) Images back to top
Antigen
Alternative Name Caspase-9 (CASP9 Antibody Abstract)
Background Caspase 9 is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspase APAF1, this step is thought to be one of the earliest in the caspase activation cascade.Synonyms: APAF-3, APAF3, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, CASP-9, CASP9, ICE-LAP6, ICE-like apoptotic protease 6, MCH6
Molecular Weight 46281 Da
Gene ID 842, 9606
UniProt P55211
Research Area Apoptosis/Necrosis, Cancer
Pathways MAPK Signaling, RTK Signaling, Apoptosis, Caspase Cascade in Apoptosis, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-Caspase 9 Antibody Target Details Caspase 9 Handling References for anti-Caspase 9 Antibody (ABIN358092) Images back to top
Application Notes ELISA: 1/1,000. Western Blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Caspase 9 Antibody Target Details Caspase 9 Application Details References for anti-Caspase 9 Antibody (ABIN358092) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-Caspase 9 Antibody (ABIN358092)

Product Details anti-Caspase 9 Antibody Target Details Caspase 9 Application Details Handling Images back to top
Background publications

Martin, Allan, Lickrish, Sampson, Morrice, Clarke: "Protein kinase A regulates caspase-9 activation by Apaf-1 downstream of cytochrome c." in: The Journal of biological chemistry, Vol. 280, Issue 15, pp. 15449-55, 2005

Raina, Pandey, Ahmad, Bharti, Ren, Kharbanda, Weichselbaum, Kufe: "c-Abl tyrosine kinase regulates caspase-9 autocleavage in the apoptotic response to DNA damage." in: The Journal of biological chemistry, Vol. 280, Issue 12, pp. 11147-51, 2005

Cornelis, Bruynooghe, Van Loo, Saelens, Vandenabeele, Beyaert: "Apoptosis of hematopoietic cells induced by growth factor withdrawal is associated with caspase-9 mediated cleavage of Raf-1." in: Oncogene, Vol. 24, Issue 9, pp. 1552-62, 2005

Mohammad, Wang, Aboukameel, Chen, Wu, Chen, Al-Katib: "Preclinical studies of a nonpeptidic small-molecule inhibitor of Bcl-2 and Bcl-X(L) [(-)-gossypol] against diffuse large cell lymphoma." in: Molecular cancer therapeutics, Vol. 4, Issue 1, pp. 13-21, 2005

Tacconi, Perri, Balestrieri, Grelli, Bernardini, Annichiarico, Mastino, Caltagirone, Macchi: "Increased caspase activation in peripheral blood mononuclear cells of patients with Alzheimer's disease." in: Experimental neurology, Vol. 190, Issue 1, pp. 254-62, 2004

Images

Product Details anti-Caspase 9 Antibody Target Details Caspase 9 Application Details Handling References for anti-Caspase 9 Antibody (ABIN358092) back to top
Supplier Images
 image for anti-Caspase 9 antibody (Caspase 9, Apoptosis-Related Cysteine Peptidase) (pSer196) (ABIN358092) anti-Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9) (pSer196) antibody
 image for anti-Caspase 9 antibody (Caspase 9, Apoptosis-Related Cysteine Peptidase) (pSer196) (ABIN358092) anti-Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9) (pSer196) antibody (Image 2)