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anti-Human Caspase 9 antibody for Flow Cytometry

Recommended Caspase 9 Antibody (supplied by: Log in to see )

Antigen
Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9) Antibodies
  • AI115399
  • APAF-3
  • APAF3
  • Apaf3
  • AW493809
  • CASP-9
  • Casp-9-CTD
  • CASP9
  • Casp9_v1
  • Caspase-9
  • Ice-Lap6
  • ICE-LAP6
  • MCH6
  • Mch6
  • PPP1R56
Epitope
Ser196
35
22
21
19
18
16
13
13
13
13
12
11
10
9
8
7
7
7
6
5
5
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
413
118
115
19
13
9
5
4
4
2
2
2
1
1
1
1
Host
Rabbit
419
47
1
Clonality
Polyclonal
Conjugate
This Caspase 9 antibody is un-conjugated
11
10
7
4
4
4
4
4
4
4
4
4
3
3
3
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
390
161
129
78
70
68
45
35
13
13
7
6
2
2
2
Supplier
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Catalog No. ABIN1881137
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010482287 ABIN2736407 ICC FACS IP IHC WB Rabbit IgG Log in to see Polyclonal
0.0010482287 ABIN3030322 FACS IHC ELISA WB Rabbit Ig Fraction Ser196 Log in to see Polyclonal
0.0010482287 ABIN2561590 FACS IF IP IHC WB Rabbit IgG Log in to see Polyclonal
0.0010482287 ABIN4288193 FACS IHC IHC (fro) IHC (p) IP Rabbit Log in to see Polyclonal
0.0010482287 ABIN4288194 FACS IHC IHC (fro) IHC (p) IP Rabbit Log in to see Polyclonal
0.0010482287 ABIN3059288 ICC FACS IF IP IHC WB Rabbit Log in to see Polyclonal
0.0010482287 ABIN3059723 ICC FACS IF IP IHC WB Rabbit Log in to see Polyclonal

Top referenced anti-Caspase 9 antibody for Flow Cytometry

Similar anti-Caspase 9 Antibodies

Application / Reactivity Human
ELISA 138 Antibodies
Enzyme Immunoassay (EIA) 6 Antibodies
Flow Cytometry (FACS) 8 Antibodies
Immunochromatography (IC) 8 Antibodies
Immunocytochemistry (ICC) 28 Antibodies
Immunofluorescence (IF) 67 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 45 Antibodies
Immunohistochemistry (IHC) 117 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 11 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 74 Antibodies
Immunoprecipitation (IP) 68 Antibodies
Intracellular Flow Cytometry (ICFC) 2 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Western Blotting (WB) 342 Antibodies

General

Antigen Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9) Antibodies
Epitope Ser196
(35), (22), (21), (19), (18), (16), (13), (13), (13), (13), (12), (11), (10), (9), (8), (7), (7), (7), (6), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(413), (118), (115), (19), (13), (9), (5), (4), (4), (2), (2), (2), (1), (1), (1), (1)
Host Rabbit
(419), (47), (1)
Clonality (Clone)
Polyclonal   ( )
Conjugate This Caspase 9 antibody is un-conjugated
(11), (10), (7), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3)
Application Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(390), (161), (129), (78), (70), (68), (45), (35), (13), (13), (7), (6), (2), (2), (2)
Pubmed 5 references available
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Product Details anti-Caspase 9 Antibody

Target Details Caspase 9 Application Details Handling References for anti-Caspase 9 Antibody (ABIN1881137) Images
Purification This antibody is purified through a protein A column, followed by peptide affinity purification. Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Immunogen This CASP9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding S196 of human CASP9.
Clone RB6899

Target Details Caspase 9

Product Details anti-Caspase 9 Antibody Application Details Handling References for anti-Caspase 9 Antibody (ABIN1881137) Images back to top
Antigen
Alternative Name CASP9 (CASP9 Antibody Abstract)
Background Caspase 9 is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspase APAF1, this step is thought to be one of the earliest in the caspase activation cascade.

Synonyms: CASP9, MCH6, Caspase-9, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, ICE-like apoptotic protease 6, Caspase-9 subunit p35, Caspase-9 subunit p10
NCBI Accession NP_001220, NP_127463, NP_001264983
UniProt P55211
Pathways MAPK Signaling, RTK Signaling, Apoptosis, Caspase Cascade in Apoptosis, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-Caspase 9 Antibody Target Details Caspase 9 Handling References for anti-Caspase 9 Antibody (ABIN1881137) Images back to top
Application Notes WB: 1:1000,IHC (p): 1:10-50,FACS: 1:10-50
Restrictions For Research Use only

Handling

Product Details anti-Caspase 9 Antibody Target Details Caspase 9 Application Details References for anti-Caspase 9 Antibody (ABIN1881137) Images back to top
Format Liquid
Concentration 0.42 mg/mL
Buffer Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

References for anti-Caspase 9 Antibody (ABIN1881137)

Product Details anti-Caspase 9 Antibody Target Details Caspase 9 Application Details Handling Images back to top
Product cited in:

Martin, Allan, Lickrish, Sampson, Morrice, Clarke: "Protein kinase A regulates caspase-9 activation by Apaf-1 downstream of cytochrome c." in: The Journal of biological chemistry, Vol. 280, Issue 15, pp. 15449-55, 2005

Raina, Pandey, Ahmad, Bharti, Ren, Kharbanda, Weichselbaum, Kufe: "c-Abl tyrosine kinase regulates caspase-9 autocleavage in the apoptotic response to DNA damage." in: The Journal of biological chemistry, Vol. 280, Issue 12, pp. 11147-51, 2005

Cornelis, Bruynooghe, Van Loo, Saelens, Vandenabeele, Beyaert: "Apoptosis of hematopoietic cells induced by growth factor withdrawal is associated with caspase-9 mediated cleavage of Raf-1." in: Oncogene, Vol. 24, Issue 9, pp. 1552-62, 2005

Mohammad, Wang, Aboukameel, Chen, Wu, Chen, Al-Katib: "Preclinical studies of a nonpeptidic small-molecule inhibitor of Bcl-2 and Bcl-X(L) [(-)-gossypol] against diffuse large cell lymphoma." in: Molecular cancer therapeutics, Vol. 4, Issue 1, pp. 13-21, 2005

Tacconi, Perri, Balestrieri, Grelli, Bernardini, Annichiarico, Mastino, Caltagirone, Macchi: "Increased caspase activation in peripheral blood mononuclear cells of patients with Alzheimer's disease." in: Experimental neurology, Vol. 190, Issue 1, pp. 254-62, 2004

Images

Product Details anti-Caspase 9 Antibody Target Details Caspase 9 Application Details Handling References for anti-Caspase 9 Antibody (ABIN1881137) back to top
Supplier Images
Western Blotting (WB) image for anti-Caspase 9 antibody (Caspase 9, Apoptosis-Related Cysteine Peptidase) (Ser196) (ABIN1881137) Western blot analysis of anti-CASP9 Antibody (S196) (ABIN1881137) in Jurkat cell line...