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anti-Human Caspase 9 antibody for Immunocytochemistry

Recommended Caspase 9 Antibody (supplied by: Log in to see )

Antigen
Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9) Antibodies
  • CASP9
  • AI115399
  • APAF-3
  • AW493809
  • CASP-9
  • Caspase-9
  • ICE-LAP6
  • Mch6
  • Apaf3
  • Casp-9-CTD
  • Casp9_v1
  • Ice-Lap6
  • APAF3
  • MCH6
  • PPP1R56
  • caspase 9
  • caspase 9, apoptosis-related cysteine peptidase
  • caspase-9-like
  • CASP9
  • LOC100101592
  • Casp9
  • LOC100518913
Reactivity
Human
411
147
140
24
14
11
7
6
6
3
2
1
Host
Mouse
408
43
1
Clonality
Monoclonal
Conjugate
This Caspase 9 antibody is un-conjugated
11
10
7
4
4
4
4
4
4
4
4
4
4
4
4
3
3
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
386
146
132
84
72
67
53
29
23
15
13
9
6
2
2
2
Supplier
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Catalog No. ABIN153015
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1886865 ICC ELISA WB Rabbit AA 299-318 Log in to see Polyclonal 1
1 ABIN1002030 ICC ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal 1
1 ABIN4288202 ELISA ICC IF IP WB Rabbit Center Log in to see Polyclonal
1 ABIN2736407 ICC FACS IP IHC WB Rabbit IgG Log in to see Polyclonal
1 ABIN462427 ICC IHC (p) ELISA WB Rabbit AA 299-318 Log in to see Polyclonal
1 ABIN1858256 ICC IHC IP WB Rabbit IgG AA 331-411 Log in to see Polyclonal
1 ABIN1858257 ICC IHC IP WB Rabbit IgG AA 1-134 Log in to see Polyclonal
1 ABIN4288196 ICC IF IHC IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN4288203 ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal
1 ABIN1732690 ICC IF WB Rabbit IgG AA 299-318 Log in to see Polyclonal
1 ABIN1031198 ICC ELISA WB Rabbit Middle Region 2 Log in to see Polyclonal
1 ABIN1732677 ICC IHC (p) IHC ELISA WB Rabbit IgG AA 299-318 Log in to see Polyclonal
1 ABIN219273 ICC IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN2618037 ICC IF IHC (p) WB Rabbit Internal Region Log in to see Polyclonal
1 ABIN2618038 ICC IF IHC (p) WB Rabbit Internal Region Log in to see Polyclonal
1 ABIN2591897 ICC IF IHC (p) WB Rabbit Internal Region Log in to see Polyclonal
1 ABIN198479 ICC IP WB Rabbit Log in to see Polyclonal
1 ABIN4903133 ICC IF IHC IP WB Rabbit IgG Log in to see
1 ABIN5013303 ICC IHC IP WB Rabbit IgG AA 331-416 Log in to see Polyclonal
1 ABIN2135499 ICC IP WB Rabbit Asp330, N-Term Log in to see Polyclonal

Top referenced anti-Caspase 9 antibody for Immunocytochemistry

Similar anti-Caspase 9 Antibodies

Application / Reactivity Human
ELISA 126 Antibodies
Enzyme Immunoassay (EIA) 6 Antibodies
Flow Cytometry (FACS) 23 Antibodies
Immunochromatography (IC) 8 Antibodies
Immunocytochemistry (ICC) 23 Antibodies
Immunofluorescence (IF) 65 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 13 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 53 Antibodies
Immunohistochemistry (IHC) 118 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 9 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 82 Antibodies
Immunoprecipitation (IP) 70 Antibodies
Intracellular Flow Cytometry (ICFC) 2 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Simple Western (SimWes) 2 Antibodies
Western Blotting (WB) 346 Antibodies

General

Antigen Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9) Antibodies
Reactivity Human
(411), (147), (140), (24), (14), (11), (7), (6), (6), (3), (2), (1)
Host Mouse
(408), (43), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Caspase 9 antibody is un-conjugated
(11), (10), (7), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(386), (146), (132), (84), (72), (67), (53), (29), (23), (15), (13), (9), (6), (2), (2), (2)
Pubmed 5 references available
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Product Details anti-Caspase 9 Antibody

Target Details Caspase 9 Application Details Handling References for anti-Caspase 9 Antibody (ABIN153015) Images
Purification Unpurified
Immunogen Recombinant human Caspase 9 pro-domain protein. [UniProt# P55211]
Clone LAP6 96-2-22
Isotype IgG1 kappa

Target Details Caspase 9

Product Details anti-Caspase 9 Antibody Application Details Handling References for anti-Caspase 9 Antibody (ABIN153015) Images back to top
Antigen
Alternative Name Caspase-9 (CASP9 Antibody Abstract)
Background Gene Symbol: CASP9
Gene ID 842
UniProt P55211
Research Area Apoptosis/Necrosis, Cancer
Pathways MAPK Signaling, RTK Signaling, Apoptosis, Caspase Cascade in Apoptosis, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Endopeptidase Activity

Application Details

Product Details anti-Caspase 9 Antibody Target Details Caspase 9 Handling References for anti-Caspase 9 Antibody (ABIN153015) Images back to top
Application Notes Western Blot 1:500-1:1000, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1:50-1:250, Immunohistochemistry-Paraffin 1:50-1:250This Caspase 9 (LAP6 96-2-22) antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot, where bands at ~46 kDa-48 kDa and 35 kDa are seen representing the pro and active forms of Caspase 9, respectively. In ICC/IF cytoplasmic staining can be seen in HeLa cells. Paraffin data from customer review.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Western protocol specific for Caspase 9 Antibody Procedure Guide for NB 500-209 Monoclonal anti-Caspase 9

Western Protocol

1) Run a 10-*15 % SDS polyacrylamide gel, loading ~20 µg of cell extract per lane.
2) Transfer the proteins to a membrane.
3) Block the membrane in PT-T20 [20 mM Tris-HCl, pH 7.4 / 150 mM NaCl/0.5 % Tween 20] + 5 % NFDM [non-fat dry milk], for 3 hour at room temperature (RT), shaking gently.
4) Rinse the membrane twice with PT-T20.
5) Incubate the membrane in anti-Caspase 9 [NB 500-209], diluted 1:500-1:1,000 in PT-T20 + 5 % NFDM, for 60 minutes at RT.
6) Wash the membrane for 15 minutes, 3 times, in PT-T20 at RT.
7) Incubate the membrane in anti-mouse conjugated to HRP (secondary antibody), diluted in PT-T20 + 5 % NFDM for 60 minutes at RT.
8) Wash the membrane for 15 minutes, 3 times, in PT-T20 at RT.
9) Develop membrane in chemiluminescent reagents, as instructed by kit-vendor.

*Positive control(s): Human kidney 293 cells

To Activate the Cell Extracts:
Bring the extract to a final concentration of 5 mM dATP.
Incubate the extracts at 37?C for 15-30 minutes.Immunocytochemistry/Immunofluorescence Protocol for Caspase 9 Antibody Immunocytochemistry ProtocolCulture cells to appropriate density in 35 mm culture dishes or 6-well plates.
1. Remove culture medium and add 10 % formalin to the dish. Fix at room temperature for 30 minutes.
. Remove the formalin and add ice cold methanol. Incubate for 5-10 minutes.
. Remove methanol and add washing solution (i.e. PBS). Be sure to not let the specimen dry out. Wash three times for 10 minutes.
. To block nonspecific antibody binding incubate in 10 % normal goat serum from 1 hour to overnight at room temperature.
. Add primary antibody at appropriate dilution and incubate at room temperature from 2 hours to overnight at room temperature.
. Remove primary antibody and replace with washing solution. Wash three times for 10 minutes.
. Add secondary antibody at appropriate dilution. Incubate for 1 hour at room temperature.
. Remove antibody and replace with wash solution, then wash for 10 minutes. Add Hoechst 33258 to wash solution at 1:25,0000 and incubate for 10 minutes. Wash a third time for 10 minutes.
. Cells can be viewed directly after washing. The plates can also be stored in PBS containing Azide covered in Parafilm (TM). Cells can also be cover-slipped using Fluoromount, with appropriate sealing.*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.
Restrictions For Research Use only

Handling

Product Details anti-Caspase 9 Antibody Target Details Caspase 9 Application Details References for anti-Caspase 9 Antibody (ABIN153015) Images back to top
Format Liquid
Concentration 30.7 mg/mL
Buffer Ascites
Buffer contains: 0.1 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -80 °C,-20 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.

References for anti-Caspase 9 Antibody (ABIN153015)

Product Details anti-Caspase 9 Antibody Target Details Caspase 9 Application Details Handling Images back to top
Product cited in:

Hsuan, Chyau, Hung, Chen, Chou: "The induction of apoptosis and autophagy by Wasabia japonica extract in colon cancer." in: European journal of nutrition, Vol. 55, Issue 2, pp. 491-503, 2016 (Sample species: Human). Further details: Western Blotting

Kim, Lee, Park, Chung, Im, Noh, Han, Moon, Jung, Ryu: "Expression of SIRT1 and apoptosis-related proteins is predictive for lymph node metastasis and disease-free survival in luminal A breast cancer." in: Virchows Archiv : an international journal of pathology, Vol. 467, Issue 5, pp. 563-70, 2015 (Sample species: Human). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Martin, Fearnhead: "Apocytochrome c blocks caspase-9 activation and Bax-induced apoptosis." in: The Journal of biological chemistry, Vol. 277, Issue 52, pp. 50834-41, 2002 (Sample species: Human). Further details: Western Blotting

Background publications

Kirsch, Doseff, Chau, Lim, de Souza-Pinto, Hansford, Kastan, Lazebnik, Hardwick: "Caspase-3-dependent cleavage of Bcl-2 promotes release of cytochrome c." in: The Journal of biological chemistry, Vol. 274, Issue 30, pp. 21155-61, 1999

Fearnhead, Rodriguez, Govek, Guo, Kobayashi, Hannon, Lazebnik: "Oncogene-dependent apoptosis is mediated by caspase-9." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 23, pp. 13664-9, 1998

Images

Product Details anti-Caspase 9 Antibody Target Details Caspase 9 Application Details Handling References for anti-Caspase 9 Antibody (ABIN153015) back to top
Supplier Images
Western Blotting (WB) image for anti-Caspase 9 antibody (Caspase 9, Apoptosis-Related Cysteine Peptidase) (ABIN153015) The pro and active forms of Caspase 9 detected in active 293 cell lysate (lane 2). La...
Western Blotting (WB) image for anti-Caspase 9 antibody (Caspase 9, Apoptosis-Related Cysteine Peptidase) (ABIN153015) Western Blot: Caspase 9 Antibody (LAP6 96-2-22) [ABIN153015] - Western blot analysis ...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Caspase 9 antibody (Caspase 9, Apoptosis-Related Cysteine Peptidase) (ABIN153015) Immunohistochemistry-Paraffin: Caspase 9 Antibody (LAP6 96-2-22) [ABIN153015] - IHC a...
Immunofluorescence (IF) image for anti-Caspase 9 antibody (Caspase 9, Apoptosis-Related Cysteine Peptidase) (ABIN153015) Immunocytochemistry/Immunofluorescence: Caspase 9 Antibody (LAP6 96-2-22) [ABIN153015...