You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Human Caspase 9 antibody for Intracellular Flow Cytometry

Recommended Caspase 9 Antibody (supplied by: Log in to see )

Antigen
Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9) Antibodies
  • AI115399
  • APAF-3
  • APAF3
  • Apaf3
  • AW493809
  • CASP-9
  • Casp-9-CTD
  • CASP9
  • Casp9_v1
  • Caspase-9
  • Ice-Lap6
  • ICE-LAP6
  • MCH6
  • Mch6
  • PPP1R56
Reactivity
Human
413
119
116
19
13
9
6
5
4
3
3
2
1
1
1
1
Host
Mouse
421
45
1
Clonality
Monoclonal
Conjugate
This Caspase 9 antibody is un-conjugated
11
10
7
4
4
4
4
4
4
4
4
4
3
3
3
Application
Intracellular Flow Cytometry (ICFC)
391
161
128
78
69
68
45
34
13
13
8
6
2
2
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN2666069
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00087393454 ABIN2666070 ICFC Mouse IgG1 kappa Log in to see 96-2-22 5

Top referenced anti-Caspase 9 antibody for Intracellular Flow Cytometry

Similar anti-Caspase 9 Antibodies

Application / Reactivity Human
ELISA 138 Antibodies
Enzyme Immunoassay (EIA) 6 Antibodies
Flow Cytometry (FACS) 8 Antibodies
Immunochromatography (IC) 8 Antibodies
Immunocytochemistry (ICC) 28 Antibodies
Immunofluorescence (IF) 67 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 45 Antibodies
Immunohistochemistry (IHC) 116 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 11 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 73 Antibodies
Immunoprecipitation (IP) 68 Antibodies
Intracellular Flow Cytometry (ICFC) 2 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Western Blotting (WB) 343 Antibodies

General

Antigen Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9) Antibodies
Reactivity Human
(413), (119), (116), (19), (13), (9), (6), (5), (4), (3), (3), (2), (1), (1), (1), (1)
Host Mouse
(421), (45), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Caspase 9 antibody is un-conjugated
(11), (10), (7), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3)
Application Intracellular Flow Cytometry (ICFC)
(391), (161), (128), (78), (69), (68), (45), (34), (13), (13), (8), (6), (2), (2), (1)
Pubmed 5 references available
Supplier Log in to see

Product Details anti-Caspase 9 Antibody

Target Details Caspase 9 Application Details Handling References for anti-Caspase 9 Antibody (ABIN2666069) Images
Cross-Reactivity (Details) recognizes 47 kD pro-caspase-9 and 37 kD cleaved caspase-9
Purification The antibody was purified by affinity chromatography.
Clone 96-2-22
Isotype IgG1 kappa

Target Details Caspase 9

Product Details anti-Caspase 9 Antibody Application Details Handling References for anti-Caspase 9 Antibody (ABIN2666069) Images back to top
Antigen
Alternative Name Caspase-9 (CASP9 Antibody Abstract)
Background Caspase 9 (also known as ICE-like apoptotic protease 6 (ICE-LAP6), apoptotic protease Mch-6, and apoptotic protease activating factor 3 (Apaf-3)) is a member of the peptidase family C14 that contains a CARD domain. This caspase is active as a heterotetramer and has been reported to have two isoforms. Pro-Caspase 9 has been reported to be approximately 47 kD. This caspase is present in the cytosol and, upon activation, translocates to the mitochondria. Caspase 9 is involved in the caspase activation cascade responsible for apoptosis execution and cleaves/activates Caspase 3 and Caspase 6. Caspase 9 is inhibited by the dominant negative isoform, Bcl-XL, c-IAP1, c-IAP2, XIAP, and Livin. This caspase becomes activated when recruited to Apaf-1/cytochrome c complex, and following cleavage by Apaf-1, granzyme B, Caspase 3, possibly Caspase 8 and Caspase 10 into large p37 and small p10 subunits. Caspase 9 interacts with BIRC7 and has been shown to cleave PARP and vimentin. The 96-2-22 monoclonal antibody has been shown to be useful for Western blotting of human caspase 9 (46 kD pro-caspase 9 as well as the 34 kD cleaved caspase 9).
Research Area Apoptosis/Necrosis, Cancer
Pathways MAPK Signaling, RTK Signaling, Apoptosis, Caspase Cascade in Apoptosis, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-Caspase 9 Antibody Target Details Caspase 9 Handling References for anti-Caspase 9 Antibody (ABIN2666069) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-Caspase 9 Antibody Target Details Caspase 9 Application Details References for anti-Caspase 9 Antibody (ABIN2666069) Images back to top
Concentration 0.5 mg/mL
Buffer phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Upon receipt, store between 2°C and 8°C.

References for anti-Caspase 9 Antibody (ABIN2666069)

Product Details anti-Caspase 9 Antibody Target Details Caspase 9 Application Details Handling Images back to top
Background publications

Frese, Frese-Schaper, Andres, Miescher, Zumkehr, Schmid: "Cardiac glycosides initiate Apo2L/TRAIL-induced apoptosis in non-small cell lung cancer cells by up-regulation of death receptors 4 and 5." in: Cancer research, Vol. 66, Issue 11, pp. 5867-74, 2006

Potokar, Milisav, Kreft, Stenovec, Zorec: "Apoptosis triggered redistribution of caspase-9 from cytoplasm to mitochondria." in: FEBS letters, Vol. 544, Issue 1-3, pp. 153-9, 2003

Sitailo, Tibudan, Denning: "Activation of caspase-9 is required for UV-induced apoptosis of human keratinocytes." in: The Journal of biological chemistry, Vol. 277, Issue 22, pp. 19346-52, 2002

Hu, Benedict, Wu, Inohara, Núñez: "Bcl-XL interacts with Apaf-1 and inhibits Apaf-1-dependent caspase-9 activation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 8, pp. 4386-91, 1998

Srinivasula, Fernandes-Alnemri, Zangrilli, Robertson, Armstrong, Wang, Trapani, Tomaselli, Litwack, Alnemri: "The Ced-3/interleukin 1beta converting enzyme-like homolog Mch6 and the lamin-cleaving enzyme Mch2alpha are substrates for the apoptotic mediator CPP32." in: The Journal of biological chemistry, Vol. 271, Issue 43, pp. 27099-106, 1996

Images

Product Details anti-Caspase 9 Antibody Target Details Caspase 9 Application Details Handling References for anti-Caspase 9 Antibody (ABIN2666069) back to top
Supplier Images
Western Blotting (WB) image for anti-Caspase 9 antibody (Caspase 9, Apoptosis-Related Cysteine Peptidase) (ABIN2666069) anti-Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9) antibody