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anti-Human Caspase 9 antibody for Immunofluorescence

Recommended Caspase 9 Antibody (supplied by: Log in to see )

Antigen
Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9) Antibodies
  • AI115399
  • APAF-3
  • APAF3
  • Apaf3
  • AW493809
  • CASP-9
  • Casp-9-CTD
  • CASP9
  • Casp9_v1
  • Caspase-9
  • Ice-Lap6
  • ICE-LAP6
  • MCH6
  • Mch6
  • PPP1R56
Epitope
Intermediate Domain 2
33
22
21
19
18
16
13
13
13
13
12
11
11
9
7
7
7
7
5
5
5
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
397
110
107
15
13
9
6
5
3
3
2
1
1
1
Host
Rabbit
404
45
1
Clonality
Polyclonal
Conjugate
This Caspase 9 antibody is un-conjugated
11
10
7
4
4
4
4
4
4
4
4
4
3
3
3
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
375
159
114
65
64
57
45
31
13
9
6
5
2
2
Supplier
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Catalog No. ABIN499579
$ 401.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.3161046 ABIN1531532 IF ELISA WB Rabbit IgG pTyr153 Log in to see Polyclonal
0.3161046 ABIN1532234 IF IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.3161046 ABIN1532550 IF ELISA WB Rabbit IgG Log in to see Polyclonal
0.3161046 ABIN1532548 IF ELISA WB Rabbit IgG Log in to see Polyclonal
0.3161046 ABIN2705695 IC IF IHC WB Rabbit Center Log in to see Polyclonal
0.3161046 ABIN2705697 IC IF IHC WB Rabbit Center Log in to see Polyclonal
0.3161046 ABIN2705696 IC IF IHC WB Rabbit Center Log in to see Polyclonal
0.3161046 ABIN615299 EIA IF IHC (p) WB Rabbit AA 299-318, N-Term Log in to see Polyclonal
0.3161046 ABIN2163271 IF ELISA WB Rabbit IgG Tyr153 Log in to see Polyclonal
0.3161046 ABIN2163278 IF ELISA WB Rabbit IgG Tyr153 Log in to see Polyclonal
0.3161046 ABIN2163274 IF ELISA WB Rabbit IgG Ser196 Log in to see Polyclonal
0.3161046 ABIN2163280 IF IHC ELISA WB Rabbit IgG Thr125 Log in to see Polyclonal
0.3161046 ABIN560175 IF PLA ELISA WB Mouse IgG1 kappa AA 1-416, full length Log in to see 3B8-4G2
0.3161046 ABIN2405536 IF WB Rabbit IgG Log in to see Polyclonal
0.0010850558 ABIN392509 IF IHC (p) WB Rabbit Ig AA 183-211, Center Log in to see Polyclonal 3
0.0010850558 ABIN1105685 IF WB Rabbit IgG AA 299-318 Log in to see Polyclonal 1
0.0010850558 ABIN393211 IF WB Mouse IgG1 kappa AA 1-417 Log in to see 3B8-4G2 5
0.0010850558 ABIN438247 ICC IF WB Mouse IgG1 kappa Log in to see LAP6 96-2-22 3
0.0010850558 ABIN271876 IF IHC (p) WB Rabbit Log in to see Polyclonal
0.0010850558 ABIN127028 IF IHC (p) IP WB Rabbit Asp330, N-Term Log in to see Polyclonal

Top referenced anti-Caspase 9 antibody for Immunofluorescence

Similar anti-Caspase 9 Antibodies

Application / Reactivity Human
ELISA 137 Antibodies
Enzyme Immunoassay (EIA) 6 Antibodies
Flow Cytometry (FACS) 6 Antibodies
Immunochromatography (IC) 8 Antibodies
Immunocytochemistry (ICC) 25 Antibodies
Immunofluorescence (IF) 64 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 45 Antibodies
Immunohistochemistry (IHC) 103 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 7 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 60 Antibodies
Immunoprecipitation (IP) 57 Antibodies
Intracellular Flow Cytometry (ICFC) 2 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Western Blotting (WB) 329 Antibodies

General

Antigen Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9) Antibodies
Epitope Intermediate Domain 2
(33), (22), (21), (19), (18), (16), (13), (13), (13), (13), (12), (11), (11), (9), (7), (7), (7), (7), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(397), (110), (107), (15), (13), (9), (6), (5), (3), (3), (2), (1), (1), (1)
Host Rabbit
(404), (45), (1)
Clonality
Polyclonal
Conjugate This Caspase 9 antibody is un-conjugated
(11), (10), (7), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3)
Application Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
(375), (159), (114), (65), (64), (57), (45), (31), (13), (9), (6), (5), (2), (2)
Pubmed 1 reference available
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Product Details anti-Caspase 9 Antibody

Target Details Caspase 9 Application Details Handling References for anti-Caspase 9 Antibody (ABIN499579) Images
Specificity Caspase-9 antibody was raised against a peptide corresponding to amino acids 299 to 318 of human caspase-9. It has no cross response to other members in caspase family.
Purification Affinity chromatography purified via peptide column
Immunogen Human Caspase-9 (Intermediate Domain 2) Peptide
Isotype IgG

Target Details Caspase 9

Product Details anti-Caspase 9 Antibody Application Details Handling References for anti-Caspase 9 Antibody (ABIN499579) Images back to top
Antigen
Alternative Name Caspase-9 (CASP9 Antibody Abstract)
Background Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. A novel member in the caspase family was recently identified and designated ICE-LAP6, Mch6, and Apaf-3 (1-3). Caspase-9 and Apaf-1 bind to each other, which leads to caspase-9 activation (3). Caspase-9 is also activated by granzyme B and CPP32 (1,2). Activated caspase-9 cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis (3). Caspase-9 play a central role in cell death induced by a wide variety of apoptosis activators including TNFalpha, TRAIL, anti-CD-95, FADD, and TRADD (4). Caspase-9 is expressed in a variety of human tissues (1,2).Synonyms: APAF-3, APAF3, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, CASP-9, CASP9, ICE-LAP6, ICE-like apoptotic protease 6, MCH6
Gene ID 842
UniProt P55211
Research Area Apoptosis/Necrosis, Cancer
Pathways MAPK Signaling, RTK Signaling, Apoptosis, Caspase Cascade in Apoptosis, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-Caspase 9 Antibody Target Details Caspase 9 Handling References for anti-Caspase 9 Antibody (ABIN499579) Images back to top
Application Notes ELISA. Western Blot: Caspase-9 antibody can be used for detection of caspase-9 at 1/1000 to1/2000 dilution. A 46 kDa band should be detected. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Caspase 9 Antibody Target Details Caspase 9 Application Details References for anti-Caspase 9 Antibody (ABIN499579) Images back to top
Buffer PBS containing 0.02 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C.

References for anti-Caspase 9 Antibody (ABIN499579)

Product Details anti-Caspase 9 Antibody Target Details Caspase 9 Application Details Handling Images back to top
Background publications

Duan, Orth, Chinnaiyan, Poirier, Froelich, He, Dixit: "ICE-LAP6, a novel member of the ICE/Ced-3 gene family, is activated by the cytotoxic T cell protease granzyme B." in: The Journal of biological chemistry, Vol. 271, Issue 28, pp. 16720-4, 1996

Images

Product Details anti-Caspase 9 Antibody Target Details Caspase 9 Application Details Handling References for anti-Caspase 9 Antibody (ABIN499579) back to top
Supplier Images
Immunofluorescence (IF) image for anti-Caspase 9 antibody (Caspase 9, Apoptosis-Related Cysteine Peptidase) (Intermediate Domain 2) (ABIN499579) Immunocytochemistry staining of K562 cells using AP30206PU-N Caspase-9 antibody at 2 ...
Western Blotting (WB) image for anti-Caspase 9 antibody (Caspase 9, Apoptosis-Related Cysteine Peptidase) (Intermediate Domain 2) (ABIN499579) Western blot analysis of caspase-9 in HeLa whole cell lysate with AP30206PU-N Caspase...